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Literature summary extracted from

  • Sonawane, V.C.
    Enzymatic modifications of cephalosporins by cephalosporin acylase and other enzymes (2006), Crit. Rev. Biotechnol., 26, 95-120.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.93 Glutaric acid inducer for cephalosporin acylase synthesis Pseudomonas sp.
3.5.1.93 Glutaric acid inducer for cephalosporin acylase synthesis Pseudomonas putida
3.5.1.93 Glutaric acid inducer for cephalosporin acylase synthesis Brevibacillus laterosporus
3.5.1.93 Glutaric acid inducer for cephalosporin acylase synthesis Sphingomonas paucimobilis
3.5.1.93 Glutaric acid inducer for cephalosporin acylase synthesis Aeromonas sp.
3.5.1.93 Glutaric acid inducer for cephalosporin acylase synthesis Flavobacterium sp.
3.5.1.93 Glutaric acid inducer for cephalosporin acylase synthesis Brevundimonas diminuta
3.5.1.93 Glutaric acid inducer for cephalosporin acylase synthesis Achromobacter xylosoxidans
3.5.1.93 Glutaric acid inducer for cephalosporin acylase synthesis Pseudomonas nitroreducens
3.5.1.93 Glutaric acid inducer for cephalosporin acylase synthesis Rhizobium viscosum
3.5.1.93 monopotassium anthroquinone 1,8-di-sulfonate
-
 Paecilomyces sp. (in: Eurotiomycetes)
3.5.1.93 N-isobutoxycarbomyl cephalosporin C
-
 Paecilomyces sp. (in: Eurotiomycetes)

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.93 expressed in Bacillus subtilis and Escherichia coli strain JM 109 Brevibacillus laterosporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.93 2.1
-
 glutaryl-7-aminocephalosporanic acid
-
 Pseudomonas sp.
3.5.1.93 2.6
-
 glutaryl-7-aminocephalosporanic acid
-
 Brevundimonas diminuta
3.5.1.93 3.2
-
 glutaryl-7-aminocephalosporanic acid
-
 Brevibacillus laterosporus
3.5.1.93 4.8
-
 cephalosporin C
-
 Brevundimonas diminuta
3.5.1.93 10.41
-
 glutaryl-7-aminocephalosporanic acid
-
 Pseudomonas nitroreducens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.93 21000
-
 1 * 35000 + 1 * 21000, SDS-PAGE Pseudomonas nitroreducens
3.5.1.93 26000
-
 1 * 58000 + 1 * 26000, SDS-PAGE Brevundimonas diminuta
3.5.1.93 35000
-
 1 * 35000 + 1 * 21000, SDS-PAGE Pseudomonas nitroreducens
3.5.1.93 58000
-
 SDS-PAGE Pseudomonas nitroreducens
3.5.1.93 58000
-
 1 * 58000 + 1 * 26000, SDS-PAGE Brevundimonas diminuta
3.5.1.93 61000
-
 1 * 61000 + 1* 28000, SDS-PAGE Pseudomonas sp.
3.5.1.93 70000
-
 SDS-PAGE Brevibacillus laterosporus
3.5.1.93 84000
-
 SDS-PAGE Brevundimonas diminuta
3.5.1.93 89000
-
 SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.93 Achromobacter xylosoxidans
-
-
-
3.5.1.93 Aeromonas sp.
-
-
-
3.5.1.93 Aeromonas sp. ACY 95
-
-
-
3.5.1.93 Arthrobacter sp.
-
-
-
3.5.1.93 Arthrobacter sp. 5-8A
-
-
-
3.5.1.93 Bacillus sp. (in: Bacteria)
-
-
-
3.5.1.93 Bacillus sp. (in: Bacteria) SV12
-
-
-
3.5.1.93 Brevibacillus laterosporus
-
-
-
3.5.1.93 Brevibacillus laterosporus J1
-
-
-
3.5.1.93 Brevundimonas diminuta
-
-
-
3.5.1.93 Brevundimonas diminuta N 176
-
-
-
3.5.1.93 Burkholderia cepacia
-
-
-
3.5.1.93 Burkholderia cepacia BY21
-
-
-
3.5.1.93 Flavobacterium sp.
-
-
-
3.5.1.93 Flavobacterium sp. 650
-
-
-
3.5.1.93 Paecilomyces sp. (in: Eurotiomycetes)
-
-
-
3.5.1.93 Pseudomonas nitroreducens
-
-
-
3.5.1.93 Pseudomonas nitroreducens CCRC 11041
-
-
-
3.5.1.93 Pseudomonas putida
-
 strain ATCC 950
-
3.5.1.93 Pseudomonas sp.
-
-
-
3.5.1.93 Pseudomonas sp. A14
-
-
-
3.5.1.93 Pseudomonas sp. BLO 72
-
-
-
3.5.1.93 Rhizobium viscosum
-
-
-
3.5.1.93 Sphingomonas paucimobilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.93 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, TEAE-cellulose chromatography, Toyopearl HW65F column chromatography, CM Toyopearl 650 column chromatography, Sephadex G-200 gel filtration, Q-Sepharose column chromatography, hydroxyapatite column chromatography, SP-Trisacryl gel filtration, and Sephacryl S-300gel filtration Arthrobacter sp.
3.5.1.93 ammonium sulfate precipitation, Toyopearl HW65F column chromatography, and CM Toyopearl 650 column chromatography Brevibacillus laterosporus
3.5.1.93 ammonium sulfate precipitation, Toyopearl HW65F column chromatography, and CM Toyopearl 650 column chromatography Brevundimonas diminuta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.93 cephalosporin C + H2O
-
 Pseudomonas sp. cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Pseudomonas putida cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Sphingomonas paucimobilis cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Aeromonas sp. cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Flavobacterium sp. cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Brevundimonas diminuta cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Achromobacter xylosoxidans cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Rhizobium viscosum cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Paecilomyces sp. (in: Eurotiomycetes) cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Aeromonas sp. ACY 95 cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Flavobacterium sp. 650 cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Pseudomonas sp. A14 cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Pseudomonas sp. BLO 72 cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 cephalosporin C + H2O
-
 Brevundimonas diminuta N 176 cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas putida 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Bacillus sp. (in: Bacteria) 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Arthrobacter sp. 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Brevibacillus laterosporus 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Sphingomonas paucimobilis 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Burkholderia cepacia 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Aeromonas sp. 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Flavobacterium sp. 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Brevundimonas diminuta 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas nitroreducens 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Rhizobium viscosum 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Paecilomyces sp. (in: Eurotiomycetes) 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Aeromonas sp. ACY 95 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Burkholderia cepacia BY21 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Arthrobacter sp. 5-8A 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Flavobacterium sp. 650 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. A14 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Brevibacillus laterosporus J1 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas nitroreducens CCRC 11041 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Bacillus sp. (in: Bacteria) SV12 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. BLO 72 7-aminocephalosporanate + glutarate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Brevundimonas diminuta N 176 7-aminocephalosporanate + glutarate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.93 dimer 1 * 35000 + 1 * 21000, SDS-PAGE Pseudomonas nitroreducens
3.5.1.93 dimer 1 * 58000 + 1 * 26000, SDS-PAGE Brevundimonas diminuta
3.5.1.93 dimer 1 * 61000 + 1* 28000, SDS-PAGE Pseudomonas sp.
3.5.1.93 monomer
-
 Brevibacillus laterosporus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.93 cephalosporin acylase
-
 Pseudomonas sp.
3.5.1.93 cephalosporin acylase
-
 Pseudomonas putida
3.5.1.93 cephalosporin acylase
-
 Sphingomonas paucimobilis
3.5.1.93 cephalosporin acylase
-
 Aeromonas sp.
3.5.1.93 cephalosporin acylase
-
 Flavobacterium sp.
3.5.1.93 cephalosporin acylase
-
 Brevundimonas diminuta
3.5.1.93 cephalosporin acylase
-
 Rhizobium viscosum
3.5.1.93 cephalosporin acylase
-
 Paecilomyces sp. (in: Eurotiomycetes)
3.5.1.93 cephalosporin acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Achromobacter xylosoxidans
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Pseudomonas sp.
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Pseudomonas putida
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Bacillus sp. (in: Bacteria)
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Arthrobacter sp.
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Brevibacillus laterosporus
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Sphingomonas paucimobilis
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Burkholderia cepacia
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Aeromonas sp.
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Flavobacterium sp.
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Brevundimonas diminuta
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Achromobacter xylosoxidans
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Pseudomonas nitroreducens
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Rhizobium viscosum
3.5.1.93 glutaryl-7-ACA acylase glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates Paecilomyces sp. (in: Eurotiomycetes)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.93 40
-
-
 Brevibacillus laterosporus
3.5.1.93 43
-
-
 Pseudomonas nitroreducens
3.5.1.93 48
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.93 9
-
 the enzyme activity sharply drops on either side of pH 9 Brevibacillus laterosporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.93 5.5
-
-
 Pseudomonas nitroreducens
3.5.1.93 9
-
-
 Brevibacillus laterosporus
3.5.1.93 9
-
-
 Brevundimonas diminuta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.93 8 10
-
 Pseudomonas sp.
3.5.1.93 8 10
-
 Brevundimonas diminuta