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Literature summary extracted from

  • Liu, L.; Wagner, C.R.; Hanna, P.E.
    Human Arylamine N-Acetyltransferase 1: In Vitro and Intracellular Inactivation by Nitrosoarene Metabolites of Toxic and Carcinogenic Arylamines (2008), Chem. Res. Toxicol., 21, 2005-2016.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.5
-
 Homo sapiens
2.3.1.5 NAT1 is recombinantly expressed Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.5 2-nitrosofluorene potent inactivator, incubation with 2-nitrosofluorene causes 91% inactivation. In the presence of a 500fold excess of glutathione (0.5 mM), inhibition is reduced to 28% Homo sapiens
2.3.1.5 2-nitrosotoluene less potent inactivator of NAT1, NAT1 with 2-nitrosotoluene causes 46% inhibition of the enzyme, whereas the presence of AcCoA lowers the extent of inhibition to 5% Homo sapiens
2.3.1.5 4-nitrosobenzene less potent inactivator of NAT1, NAT1 with nitrosobenzene causes 59% inhibition of the enzyme, whereas the presence of AcCoA lowers the extent of inhibition to 13% Homo sapiens
2.3.1.5 4-nitrosobiphenyl potent inactivator, incubation with 4-nitrosobiphenyl causes 71% inactivation. In the presence of a 500fold excess of glutathione (0.5 mM), inhibition is reduced to 35% Homo sapiens
2.3.1.5 nitrosobenzene
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.5 cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.5 35000
-
 HeLa cell NAT1, determined by SDS-PAGE and Western blot analysis Homo sapiens
2.3.1.5 37000
-
 recombinant NAT1, determined by SDS-PAGE and Western blot analysis Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.5
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.5 HeLa cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.5 0.00161
-
 HeLa cell + N-acetyl-L-cysteine + 4-nitrosobiphenyl Homo sapiens
2.3.1.5 0.00172
-
 HeLa cell + 4-nitrosobiphenyl Homo sapiens
2.3.1.5 0.00408
-
 in HeLa cells, substrate: p-aminosalicylic acid Homo sapiens
2.3.1.5 0.0044
-
 in HeLa cells, substrate: p-aminobenzoic acid Homo sapiens
2.3.1.5 0.00454
-
 HeLa cell + N-acetyl-L-cysteine Homo sapiens
2.3.1.5 0.00466
-
 HeLa cell Homo sapiens
2.3.1.5 0.237
-
 recombinant NAT1, substrate: p-aminobenzoic acid Homo sapiens
2.3.1.5 0.337
-
 recombinant NAT1, substrate: p-aminosalicylic acid Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 acetyl-CoA + 2-aminobenzoic acid
-
 Homo sapiens CoA + N-acetyl-2-aminobenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + p-aminosalicylic acid
-
 Homo sapiens CoA + N-acetyl-4-aminosalicylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 arylamine N-acetyltransferase
-
 Homo sapiens
2.3.1.5 arylamine N-acetyltransferase 1
-
 Homo sapiens
2.3.1.5 NAT1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.5 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.5 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.5 acetyl-CoA
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.5 0.00067
-
 4-nitrosobiphenyl
-
 Homo sapiens
2.3.1.5 0.00067
-
 4-nitrosobiphenyl recombinant NAT1 Homo sapiens
2.3.1.5 0.54
-
 4-nitrosobenzene
-
 Homo sapiens
2.3.1.5 0.54
-
 nitrosobenzene recombinant NAT1 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.5 0.00004
-
-
 Homo sapiens 2-nitrosofluorene
2.3.1.5 0.00004
-
 HeLa cell NAT1 Homo sapiens 2-nitrosofluorene
2.3.1.5 0.00006
-
-
 Homo sapiens 4-nitrosobiphenyl
2.3.1.5 0.00006
-
 HeLa cell NAT1 Homo sapiens 4-nitrosobiphenyl
2.3.1.5 0.091
-
-
 Homo sapiens 2-nitrosotoluene
2.3.1.5 0.091
-
 HeLa cell NAT1 Homo sapiens 2-nitrosotoluene
2.3.1.5 0.237
-
-
 Homo sapiens 4-nitrosobenzene
2.3.1.5 0.237
-
 HeLa cell NAT1 Homo sapiens nitrosobenzene