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Literature summary extracted from

  • Liu, L.; Von Vett, A.; Zhang, N.; Walters, K.J.; Wagner, C.R.; Hanna, P.E.
    Arylamine N-acetyltransferases: characterization of the substrate specificities and molecular interactions of environmental arylamines with human NAT1 and NAT2 (2007), Chem. Res. Toxicol., 20, 1300-1308.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.5 human wild-type NAT2 is overexpressed as a dihydrofolate reductase fusion protein containing a TEV protease-sensitive linker Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.5 0.059
-
 p-Aminosalicylic acid
-
 Homo sapiens
2.3.1.5 0.084
-
 p-Aminobenzoic acid
-
 Homo sapiens
2.3.1.5 0.109
-
 2-Aminofluorene
-
 Homo sapiens
2.3.1.5 0.191
-
 4-aminobiphenyl
-
 Homo sapiens
2.3.1.5 0.205
-
 4-ethylaniline
-
 Homo sapiens
2.3.1.5 0.28
-
 3,5-dimethylaniline
-
 Homo sapiens
2.3.1.5 0.286
-
 2-Aminofluorene
-
 Homo sapiens
2.3.1.5 0.352
-
 3,4-dimethylaniline
-
 Homo sapiens
2.3.1.5 0.483
-
 4-methylaniline
-
 Homo sapiens
2.3.1.5 0.486
-
 4-aminobiphenyl
-
 Homo sapiens
2.3.1.5 0.576
-
 3-ethylaniline
-
 Homo sapiens
2.3.1.5 0.688
-
 3,4-dimethylaniline
-
 Homo sapiens
2.3.1.5 0.742
-
 3,5-dimethylaniline
-
 Homo sapiens
2.3.1.5 1.11
-
 2,3-dimethylaniline
-
 Homo sapiens
2.3.1.5 1.32
-
 3-ethylaniline
-
 Homo sapiens
2.3.1.5 2.06
-
 2,4-dimethylaniline
-
 Homo sapiens
2.3.1.5 2.11
-
 2-ethylaniline
-
 Homo sapiens
2.3.1.5 2.32
-
 2-methylaniline
-
 Homo sapiens
2.3.1.5 2.49
-
 aniline
-
 Homo sapiens
2.3.1.5 3.12
-
 2,5-dimethylaniline
-
 Homo sapiens
2.3.1.5 3.27
-
 4-ethylaniline
-
 Homo sapiens
2.3.1.5 5.03
-
 2,4-dimethylaniline
-
 Homo sapiens
2.3.1.5 5.39
-
 sulfamethazine
-
 Homo sapiens
2.3.1.5 5.39
-
 p-Aminosalicylic acid
-
 Homo sapiens
2.3.1.5 5.53
-
 2,3-dimethylaniline
-
 Homo sapiens
2.3.1.5 6.73
-
 2,5-dimethylaniline
-
 Homo sapiens
2.3.1.5 7.18
-
 2-methylaniline
-
 Homo sapiens
2.3.1.5 112
-
 aniline
-
 Homo sapiens
2.3.1.5 118
-
 4-methylaniline
-
 Homo sapiens
2.3.1.5 152
-
 p-Aminobenzoic acid
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.5 33000
-
 SDS-PAGE Homo sapiens
2.3.1.5 33840
-
 mass spectrometry Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.5 by using chromatographic purification. Yield: 2.8 mg of homogeneous NAT2 from 2 L of cell culture Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 acetyl-CoA + 2,3-dimethylaniline
-
 Homo sapiens CoA + N-acetyl-2,3-dimethylaniline
-
 ?
2.3.1.5 acetyl-CoA + 2,4-dimethylaniline
-
 Homo sapiens CoA + N-acetyl-2,4-dimethylaniline
-
 ?
2.3.1.5 acetyl-CoA + 2,5-dimethylaniline
-
 Homo sapiens CoA + N-acetyl-2,5-dimethylaniline
-
 ?
2.3.1.5 acetyl-CoA + 2-aminofluorene
-
 Homo sapiens CoA + N-acetyl-2-aminofluorene
-
 ?
2.3.1.5 acetyl-CoA + 2-ethylaniline
-
 Homo sapiens CoA + N-acetyl-2-ethylaniline
-
 ?
2.3.1.5 acetyl-CoA + 2-methylaniline
-
 Homo sapiens CoA + N-acetyl-2-methylaniline
-
 ?
2.3.1.5 acetyl-CoA + 3,4-dimethylaniline
-
 Homo sapiens CoA + N-acetyl-3,4-dimethylaniline
-
 ?
2.3.1.5 acetyl-CoA + 3,5-dimethylaniline
-
 Homo sapiens CoA + N-acetyl-3,5-dimethylaniline
-
 ?
2.3.1.5 acetyl-CoA + 3-ethylaniline
-
 Homo sapiens CoA + N-acetyl-3-ethylaniline
-
 ?
2.3.1.5 acetyl-CoA + 4-aminobiphenyl
-
 Homo sapiens CoA + N-acetyl-4-aminobiphenyl
-
 ?
2.3.1.5 acetyl-CoA + 4-ethylaniline
-
 Homo sapiens CoA + N-acetyl-4-ethylaniline
-
 ?
2.3.1.5 acetyl-CoA + 4-methylaniline
-
 Homo sapiens CoA + N-acetyl-4-methylaniline
-
 ?
2.3.1.5 acetyl-CoA + aniline
-
 Homo sapiens CoA + N-acetyl-aniline
-
 ?
2.3.1.5 acetyl-CoA + p-aminobenzoic acid
-
 Homo sapiens CoA + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 acetyl-CoA + p-aminosalicylic acid
-
 Homo sapiens CoA + N-acetyl-4-aminosalicylic acid
-
 ?
2.3.1.5 acetyl-CoA + sulfamethazine
-
 Homo sapiens CoA + N-acetyl-sulfamethazine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 arylamine N-acetyltransferase 1
-
 Homo sapiens
2.3.1.5 NAT1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.5 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.5 5.7
-
 2,5-dimethylaniline
-
 Homo sapiens
2.3.1.5 12
-
 2-methylaniline
-
 Homo sapiens
2.3.1.5 27
-
 p-Aminosalicylic acid
-
 Homo sapiens
2.3.1.5 27
-
 2,3-dimethylaniline
-
 Homo sapiens
2.3.1.5 38
-
 p-Aminobenzoic acid
-
 Homo sapiens
2.3.1.5 61
-
 2-ethylaniline
-
 Homo sapiens
2.3.1.5 67
-
 2,4-dimethylaniline
-
 Homo sapiens
2.3.1.5 111
-
 2-methylaniline
-
 Homo sapiens
2.3.1.5 243
-
 4-aminobiphenyl
-
 Homo sapiens
2.3.1.5 256
-
 4-aminobiphenyl
-
 Homo sapiens
2.3.1.5 256
-
 2,3-dimethylaniline
-
 Homo sapiens
2.3.1.5 281
-
 aniline
-
 Homo sapiens
2.3.1.5 298
-
 p-Aminobenzoic acid
-
 Homo sapiens
2.3.1.5 303
-
 4-methylaniline
-
 Homo sapiens
2.3.1.5 308
-
 3,5-dimethylaniline
-
 Homo sapiens
2.3.1.5 310
-
 3-ethylaniline
-
 Homo sapiens
2.3.1.5 313
-
 2,5-dimethylaniline
-
 Homo sapiens
2.3.1.5 387
-
 sulfamethazine
-
 Homo sapiens
2.3.1.5 430
-
 4-ethylaniline
-
 Homo sapiens
2.3.1.5 449
-
 2-Aminofluorene
-
 Homo sapiens
2.3.1.5 461
-
 3,4-dimethylaniline
-
 Homo sapiens
2.3.1.5 591
-
 p-Aminosalicylic acid
-
 Homo sapiens
2.3.1.5 661
-
 2,4-dimethylaniline
-
 Homo sapiens
2.3.1.5 700
-
 4-ethylaniline
-
 Homo sapiens
2.3.1.5 711
-
 aniline
-
 Homo sapiens
2.3.1.5 759
-
 2-Aminofluorene
-
 Homo sapiens
2.3.1.5 800
-
 3,4-dimethylaniline
-
 Homo sapiens
2.3.1.5 1220
-
 3,5-dimethylaniline
-
 Homo sapiens
2.3.1.5 1600
-
 4-methylaniline
-
 Homo sapiens
2.3.1.5 1960
-
 3-ethylaniline
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.5 7
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.5 acetyl-CoA
-
 Homo sapiens