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Literature summary extracted from

  • Cai, J.; Yang, J.; Du, Y.; Fan, L.; Qiu, Y.; Li, J.; Kennedy, J.F.
    Purification and characterization of chitin deacetylase from Scopulariopsis brevicaulis (2006), Carbohydr. Polym., 65, 211-217.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.41 extracellular
-
 Scopulariopsis brevicaulis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.41 55000
-
 gel filtration Scopulariopsis brevicaulis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Scopulariopsis brevicaulis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.41
-
 Scopulariopsis brevicaulis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.41 culture condition chitin as sole carbon resource of culture medium. The enzyme activity is 10-11 units/ml culture supernatant after the strain is shaken at 200 rpm and 29°C for 96 h Scopulariopsis brevicaulis
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.41 2.58
-
 substrate: hexa-N-acetylchitohexaose Scopulariopsis brevicaulis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 chitin + H2O the enzyme has high deacetylating activity on amorphous chitin from Aspergillus niger mycelium (37% deacylation) and water-soluble chitosan (33%) but low activity on shrimp crystalline chitin (3.7%) Scopulariopsis brevicaulis chitosan + acetate
-
 ?
3.5.1.41 chitooligosaccharides + H2O active on chitooligosaccharides with at least two N-acetyl-glucosamine residues, but the activity increased with the number of N-acetyl-glucosamine residues Scopulariopsis brevicaulis ?
-
 ?
3.5.1.41 GlcNAc-beta-1,4-GlcNAc + H2O
-
 Scopulariopsis brevicaulis ?
-
 ?
3.5.1.41 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O
-
 Scopulariopsis brevicaulis ?
-
 ?
3.5.1.41 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O
-
 Scopulariopsis brevicaulis ?
-
 ?
3.5.1.41 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O
-
 Scopulariopsis brevicaulis ?
-
 ?
3.5.1.41 GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1,4-GlcNAc + H2O
-
 Scopulariopsis brevicaulis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.41 monomer 1 * 55000, SDS-PAGE Scopulariopsis brevicaulis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.41 55
-
 substrate: hexa-N-acetylchitohexaose Scopulariopsis brevicaulis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.41 35 65 35°C: about 45% of maximal activity, 65°C: about 40% of maximal activity Scopulariopsis brevicaulis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.41 55
-
 1 h, stable up to Scopulariopsis brevicaulis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.41 7.5
-
 substrate: hexa-N-acetylchitohexaose Scopulariopsis brevicaulis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.41 6 10 pH 6: about 50% of maximal activity, pH 10: about 55% of maximal activity Scopulariopsis brevicaulis