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Literature summary extracted from

  • Kehrer, D.; Ahmed, H.; Brinkmann, H.; Siebers, B.
    Glycerate kinase of the hyperthermophilic archaeon Thermoproteus tenax: new insights into the phylogenetic distribution and physiological role of members of the three different glycerate kinase classes (2007), BMC Genomics, 8, 301.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.165 expression in Escherichia coli Thermoproteus tenax

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.165 (R)-glycerate substrate inhibition at high glycerate concentrations Thermoproteus tenax
2.7.1.165 ADP competitive inhibition Thermoproteus tenax
2.7.1.165 EDTA 40 mM Thermoproteus tenax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.165 0.02
-
 (R)-glycerate
-
 Thermoproteus tenax
2.7.1.165 0.03
-
 ATP
-
 Thermoproteus tenax

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.165 Ca2+ 15% of the activity with Mg2+ Thermoproteus tenax
2.7.1.165 Co2+ 56% of the activity with Mg2+ Thermoproteus tenax
2.7.1.165 Mg2+ highest activity is observed in presence of 20 mM Mg2+ Thermoproteus tenax
2.7.1.165 Mn2+ 59% of the activity with Mg2+ Thermoproteus tenax
2.7.1.165 additional information no activity is detected in the presence of Cu2+ and Fe2+ at 0.2, 2, 5 and 20 mM, respectively Thermoproteus tenax
2.7.1.165 Ni2+ 30% of the activity with Mg2+ Thermoproteus tenax

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.165 44000
-
 1 * 44000, SDS-PAGE Thermoproteus tenax
2.7.1.165 48300
-
 gel filtration Thermoproteus tenax

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.165 ATP + (R)-glycerate Thermoproteus tenax enzyme of the branched Entner-Doudoroff pathway ADP + 2-phospho-(R)-glycerate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.165 Thermoproteus tenax Q703V9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.165
-
 Thermoproteus tenax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.165 ATP + (R)-glycerate
-
 Thermoproteus tenax ADP + 2-phospho-(R)-glycerate
-
 ?
2.7.1.165 ATP + (R)-glycerate enzyme of the branched Entner-Doudoroff pathway Thermoproteus tenax ADP + 2-phospho-(R)-glycerate
-
 ?
2.7.1.165 CTP + (R)-glycerate 16% of the activity with ATP Thermoproteus tenax CDP + 2-phospho-(R)-glycerate
-
 ?
2.7.1.165 GTP + (R)-glycerate 20% of the activity with ATP Thermoproteus tenax GDP + 2-phospho-(R)-glycerate
-
 ?
2.7.1.165 additional information no activity is observed with ADP, diphosphate and polyphosphates Thermoproteus tenax ?
-
 ?
2.7.1.165 TTP + (R)-glycerate 16% of the activity with ATP Thermoproteus tenax TDP + 2-phospho-(R)-glycerate
-
 ?
2.7.1.165 UTP + (R)-glycerate 10% of the activity with ATP Thermoproteus tenax UDP + 2-phospho-(R)-glycerate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.165 monomer 1 * 44000, SDS-PAGE Thermoproteus tenax

Synonyms

EC Number Synonyms Comment Organism
2.7.1.165 garK
-
 Thermoproteus tenax

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.165 90
-
-
 Thermoproteus tenax

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.165 50 90 50°C: 15% of maximal activity, 70°C: 55% of maximal activity, 90°C: maximal activity Thermoproteus tenax

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.165 3.1
-
 ATP
-
 Thermoproteus tenax
2.7.1.165 3.6
-
 D-glycerate
-
 Thermoproteus tenax