EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.1 | arginine | - |
Mus musculus | |
2.3.1.1 | arginine | - |
Xenopus laevis | |
2.3.1.1 | arginine | - |
Xenopus tropicalis | |
2.3.1.35 | L-arginine | - |
Mus musculus |
EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.1.1 | additional information | N-acetylglutamate synthase is a molecular marker for evolution of tetrapods | Pseudomonas aeruginosa |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.1 | cloned in Escherichia coli as a His-tagged fusion protein | Mus musculus |
2.3.1.1 | cloned in Escherichia coli as a His-tagged fusion protein | Escherichia coli |
2.3.1.1 | cloned in Escherichia coli as a His-tagged fusion protein | Arabidopsis thaliana |
2.3.1.1 | cloned in Escherichia coli as a His-tagged fusion protein | Xenopus laevis |
2.3.1.1 | cloned in Escherichia coli as a His-tagged fusion protein | Xanthomonas campestris |
2.3.1.1 | cloned in Escherichia coli as a His-tagged fusion protein | Danio rerio |
2.3.1.1 | cloned in Escherichia coli as a His-tagged fusion protein | Takifugu rubripes |
2.3.1.1 | cloned in Escherichia coli as a His-tagged fusion protein | Xenopus tropicalis |
2.3.1.1 | cloned in Escherichia coli as a His-tagged fusion protein | Maricaulis maris |
2.3.1.1 | phylogenetic analysis | Pseudomonas aeruginosa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.1 | E280A | specific activity (micromol/min/mg): 26.93, 26.68 (+ 1mM arginine), arginine inhibition is abolished | Xanthomonas campestris |
2.3.1.1 | E280A | specific activity (micromol/min/mg): 26.93, 26.68 (+1mM arginine), arginine inhibition is abolished | Xanthomonas campestris |
2.3.1.1 | E354A | specific activity (micromol/min/mg): 15.47, 15.28 (+ 1mM arginine), arginine activation is abolished | Mus musculus |
2.3.1.1 | E354A | specific activity (micromol/min/mg): 15.47, 15.28 (+1mM arginine), arginine activation is abolished | Mus musculus |
2.3.1.1 | F121C | specific activity (micromol/min/mg): 12.22, 13.08 (+ 1mM arginine), arginine activation is abolished | Mus musculus |
2.3.1.1 | F121C | specific activity (micromol/min/mg): 12.22, 13.08 (+1mM arginine), arginine activation is abolished | Mus musculus |
2.3.1.1 | F35C | specific activity (micromol/min/mg): 9.66, 4.31 (+ 1mM arginine), only partial inhibition by arginine | Xanthomonas campestris |
2.3.1.1 | F35C | specific activity (micromol/min/mg): 9.66, 4.31 (+1mM arginine), only partial inhibition by arginine | Xanthomonas campestris |
2.3.1.1 | G286P | specific activity (micromol/min/mg): 3.51, 3.39 (+ 1mM arginine), no inhibition by arginine | Xanthomonas campestris |
2.3.1.1 | G286P | specific activity (micromol/min/mg): 3.51, 3.39 (+1mM arginine), no inhibition by arginine | Xanthomonas campestris |
2.3.1.1 | G288S | specific activity (micromol/min/mg): 46.04, 42.55 (+ 1mM arginine), no inhibition by arginine | Xanthomonas campestris |
2.3.1.1 | G288S | specific activity (micromol/min/mg): 46.04, 42.55 (+1mM arginine), no inhibition by arginine | Xanthomonas campestris |
2.3.1.1 | G360P | specific activity (micromol/min/mg): 13.00, 12.57 (+ 1mM arginine), arginine activation is abolished | Mus musculus |
2.3.1.1 | G360P | specific activity (micromol/min/mg): 13.00, 12.57 (+1mM arginine), arginine activation is abolished | Mus musculus |
2.3.1.1 | G362S | specific activity (micromol/min/mg): 3.22, 3.19 (+ 1mM arginine), arginine activation is abolished | Mus musculus |
2.3.1.1 | G362S | specific activity (micromol/min/mg): 3.22, 3.19 (+1mM arginine), arginine activation is abolished | Mus musculus |
2.3.1.35 | E280A | change of resiude to the corresponding Escherichia coli residue. Mutation abolishes arginine inhibition and decreases synthase activity | Xanthomonas campestris |
2.3.1.35 | E354A | change of residue to the corresponding Escherichia coli residue. Mutation abolishes arginine activation | Mus musculus |
2.3.1.35 | F121C | change of residue to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation abolishes arginine activation | Mus musculus |
2.3.1.35 | F35C | change of resiude to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation leads to partial inhibition of both synthase and kinase activities by arginine and decrease in synthase activity | Xanthomonas campestris |
2.3.1.35 | G286P | change of resiude to the corresponding Escherichia coli residue. Mutation abolishes arginine inhibition and decreases synthase activity | Xanthomonas campestris |
2.3.1.35 | G288S | change of resiude to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation abolishes arginine inhibition and decreases synthase activity | Xanthomonas campestris |
2.3.1.35 | G360P | change of residue to the corresponding Escherichia coli residue. Mutation abolishes arginine activation | Mus musculus |
2.3.1.35 | G362S | change of residue to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation abolishes arginine activation | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.1 | arginine | - |
Arabidopsis thaliana | |
2.3.1.1 | arginine | - |
Danio rerio | |
2.3.1.1 | arginine | - |
Escherichia coli | |
2.3.1.1 | arginine | - |
Maricaulis maris | |
2.3.1.1 | arginine | the arginine binding site is localized in the kinase domain. Arginine is an allosteric inhibitor of microbial NAGS as part of feedback regulation of arginine biosynthesis, arginine is also an inhibitor of plant NAGS, a partial inhibitor of fish NAGS, but an allosteric activator of mammalian NAGS | Pseudomonas aeruginosa | |
2.3.1.1 | arginine | - |
Takifugu rubripes | |
2.3.1.1 | arginine | - |
Xanthomonas campestris | |
2.3.1.35 | arginine | - |
Xanthomonas campestris |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.1 | acetyl-CoA + L-glutamate | Pseudomonas aeruginosa | NAGS is the first enzyme of arginine biosynthesis, microbial arginine biosynthesis pathway, overview. Arginine is an allosteric inhibitor of microbial NAGS, arginine is also an inhibitor of plant NAGS, a partial inhibitor of fish NAGS, but an allosteric activator of mammalian NAGS | CoA + N-acetyl-L-glutamate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.1 | Arabidopsis thaliana | - |
- |
- |
2.3.1.1 | Danio rerio | - |
- |
- |
2.3.1.1 | Escherichia coli | - |
- |
- |
2.3.1.1 | Maricaulis maris | - |
- |
- |
2.3.1.1 | Mus musculus | - |
- |
- |
2.3.1.1 | Pseudomonas aeruginosa | P22567 | - |
- |
2.3.1.1 | Takifugu rubripes | - |
- |
- |
2.3.1.1 | Xanthomonas campestris | - |
- |
- |
2.3.1.1 | Xenopus laevis | - |
- |
- |
2.3.1.1 | Xenopus tropicalis | - |
- |
- |
2.3.1.35 | Mus musculus | - |
- |
- |
2.3.1.35 | Xanthomonas campestris | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.1 | using Ni-NTA chromatography | Mus musculus |
2.3.1.1 | using Ni-NTA chromatography | Escherichia coli |
2.3.1.1 | using Ni-NTA chromatography | Arabidopsis thaliana |
2.3.1.1 | using Ni-NTA chromatography | Xenopus laevis |
2.3.1.1 | using Ni-NTA chromatography | Xanthomonas campestris |
2.3.1.1 | using Ni-NTA chromatography | Danio rerio |
2.3.1.1 | using Ni-NTA chromatography | Takifugu rubripes |
2.3.1.1 | using Ni-NTA chromatography | Xenopus tropicalis |
2.3.1.1 | using Ni-NTA chromatography | Maricaulis maris |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.1 | additional information | - |
no activity detectable upon addition of 1 mM arginine | Arabidopsis thaliana |
2.3.1.1 | additional information | - |
no activity detectable upon addition of 1 mM arginine | Xanthomonas campestris |
2.3.1.1 | 0.098 | - |
wild-type | Xenopus tropicalis |
2.3.1.1 | 0.136 | - |
wild-type + 1 mM arginine | Xenopus tropicalis |
2.3.1.1 | 0.438 | - |
wild-type | Xenopus laevis |
2.3.1.1 | 0.528 | - |
wild-type + 1 mM arginine | Xenopus laevis |
2.3.1.1 | 0.572 | - |
wild-type + 1mM arginine | Maricaulis maris |
2.3.1.1 | 1.84 | - |
wild-type + 1mM arginine | Escherichia coli |
2.3.1.1 | 3.19 | - |
mutant G362S + 1 mM arginine | Mus musculus |
2.3.1.1 | 3.22 | - |
mutant G362S | Mus musculus |
2.3.1.1 | 3.39 | - |
mutant G286P + 1 mM arginine | Xanthomonas campestris |
2.3.1.1 | 3.39 | - |
mutant G286P + 1mM arginine | Xanthomonas campestris |
2.3.1.1 | 3.51 | - |
mutant G286P | Xanthomonas campestris |
2.3.1.1 | 4.31 | - |
mutant F35C + 1 mM arginine | Xanthomonas campestris |
2.3.1.1 | 4.31 | - |
mutant F35C + 1mM arginine | Xanthomonas campestris |
2.3.1.1 | 5.475 | - |
wild-type + 1 mM arginine | Danio rerio |
2.3.1.1 | 5.85 | - |
wild-type | Escherichia coli |
2.3.1.1 | 9.11 | - |
wild-type | Arabidopsis thaliana |
2.3.1.1 | 9.66 | - |
mutant F35C | Xanthomonas campestris |
2.3.1.1 | 10.64 | - |
wild-type | Danio rerio |
2.3.1.1 | 12.22 | - |
mutant F121C | Mus musculus |
2.3.1.1 | 12.27 | - |
wild-type | Mus musculus |
2.3.1.1 | 12.57 | - |
mutant G360P + 1 mM arginine | Mus musculus |
2.3.1.1 | 12.57 | - |
mutant G360P + 1mM arginine | Mus musculus |
2.3.1.1 | 13 | - |
mutant G360P | Mus musculus |
2.3.1.1 | 13.08 | - |
mutant F121C + 1 mM arginine | Mus musculus |
2.3.1.1 | 15.28 | - |
mutant E354A + 1 mM arginine | Mus musculus |
2.3.1.1 | 15.47 | - |
mutant E354A | Mus musculus |
2.3.1.1 | 15.89 | - |
wild-type + 1 mM arginine | Takifugu rubripes |
2.3.1.1 | 20.88 | - |
wild-type | Takifugu rubripes |
2.3.1.1 | 25.56 | - |
wild-type + 1 mM arginine | Mus musculus |
2.3.1.1 | 26.68 | - |
mutant E280A + 1 mM arginine | Xanthomonas campestris |
2.3.1.1 | 26.68 | - |
mutant E280A + 1mM arginine | Xanthomonas campestris |
2.3.1.1 | 26.93 | - |
mutant E280A | Xanthomonas campestris |
2.3.1.1 | 42.55 | - |
mutant G288S + 1 mM arginine | Xanthomonas campestris |
2.3.1.1 | 42.55 | - |
mutant G288S + 1mM arginine | Xanthomonas campestris |
2.3.1.1 | 46.04 | - |
mutant G288S | Xanthomonas campestris |
2.3.1.1 | 62 | - |
wild-type | Maricaulis maris |
2.3.1.1 | 71 | - |
wild-type | Xanthomonas campestris |
2.3.1.1 | 111.7 | - |
wild-type | Xanthomonas campestris |
2.3.1.35 | 3.2 | - |
mutant G362S | Mus musculus |
2.3.1.35 | 3.2 | - |
mutant G362S, presence of 1 mM L-arginine | Mus musculus |
2.3.1.35 | 3.4 | - |
mutant G286P, presence of 1 mM L-arginine | Xanthomonas campestris |
2.3.1.35 | 3.5 | - |
mutant G286P | Xanthomonas campestris |
2.3.1.35 | 4.3 | - |
mutant F35C, presence of 1 mM L-arginine | Xanthomonas campestris |
2.3.1.35 | 9.7 | - |
mutant F35C | Xanthomonas campestris |
2.3.1.35 | 12.2 | - |
mutant F121C | Mus musculus |
2.3.1.35 | 12.3 | - |
wild-type | Mus musculus |
2.3.1.35 | 12.3 | - |
wild-type, presence of 1 mM L-arginine | Mus musculus |
2.3.1.35 | 12.6 | - |
mutant G360P, presence of 1 mM L-arginine | Mus musculus |
2.3.1.35 | 13 | - |
mutant G360P | Mus musculus |
2.3.1.35 | 13.1 | - |
mutant E354A, presence of 1 mM L-arginine | Mus musculus |
2.3.1.35 | 15.3 | - |
mutant F121C, presence of 1 mM L-arginine | Mus musculus |
2.3.1.35 | 15.5 | - |
mutant E354A | Mus musculus |
2.3.1.35 | 26.7 | - |
mutant E280A, presence of 1 mM L-arginine | Xanthomonas campestris |
2.3.1.35 | 26.9 | - |
mutant E280A | Xanthomonas campestris |
2.3.1.35 | 42.6 | - |
mutant G288S, presence of 1 mM L-arginine | Xanthomonas campestris |
2.3.1.35 | 46 | - |
mutant G288S | Xanthomonas campestris |
2.3.1.35 | 71 | - |
wild-type | Xanthomonas campestris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.1 | acetyl-CoA + L-glutamate | - |
Pseudomonas aeruginosa | CoA + N-acetyl-L-glutamate | - |
? | |
2.3.1.1 | acetyl-CoA + L-glutamate | NAGS is the first enzyme of arginine biosynthesis, microbial arginine biosynthesis pathway, overview. Arginine is an allosteric inhibitor of microbial NAGS, arginine is also an inhibitor of plant NAGS, a partial inhibitor of fish NAGS, but an allosteric activator of mammalian NAGS | Pseudomonas aeruginosa | CoA + N-acetyl-L-glutamate | - |
? | |
2.3.1.35 | N2-acetyl-L-ornithine + L-glutamate | - |
Mus musculus | L-ornithine + N-acetyl-L-glutamate | - |
? | |
2.3.1.35 | N2-acetyl-L-ornithine + L-glutamate | - |
Xanthomonas campestris | L-ornithine + N-acetyl-L-glutamate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.1 | N-acetylglutamate synthase | - |
Mus musculus |
2.3.1.1 | N-acetylglutamate synthase | - |
Escherichia coli |
2.3.1.1 | N-acetylglutamate synthase | - |
Arabidopsis thaliana |
2.3.1.1 | N-acetylglutamate synthase | - |
Xenopus laevis |
2.3.1.1 | N-acetylglutamate synthase | - |
Xanthomonas campestris |
2.3.1.1 | N-acetylglutamate synthase | - |
Danio rerio |
2.3.1.1 | N-acetylglutamate synthase | - |
Takifugu rubripes |
2.3.1.1 | N-acetylglutamate synthase | - |
Xenopus tropicalis |
2.3.1.1 | N-acetylglutamate synthase | - |
Maricaulis maris |
2.3.1.1 | N-acetylglutamate synthase | - |
Pseudomonas aeruginosa |
2.3.1.1 | NAGS | - |
Mus musculus |
2.3.1.1 | NAGS | - |
Escherichia coli |
2.3.1.1 | NAGS | - |
Arabidopsis thaliana |
2.3.1.1 | NAGS | - |
Xenopus laevis |
2.3.1.1 | NAGS | - |
Xanthomonas campestris |
2.3.1.1 | NAGS | - |
Danio rerio |
2.3.1.1 | NAGS | - |
Takifugu rubripes |
2.3.1.1 | NAGS | - |
Xenopus tropicalis |
2.3.1.1 | NAGS | - |
Maricaulis maris |
2.3.1.1 | NAGS | - |
Pseudomonas aeruginosa |
2.3.1.1 | XcNAGS | - |
Xanthomonas campestris |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.1 | acetyl-CoA | - |
Pseudomonas aeruginosa |