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Literature summary extracted from

  • Lee, J.Y.; Cheong, D.E.; Kim, G.J.
    A novel assay system for the measurement of transketolase activity using xylulokinase from Saccharomyces cerevisiae (2008), Biotechnol. Lett., 30, 899-904.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.2.1.1 analysis highly effective, stable and sensitive method for measuring TKT activity, incorporating xylulokinase, which induces generation of xylulose 5-phosphate from xylulose, from Saccharomyces cerevisiae into conventional TKT assay Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.17 expressed in Escherichia coli strain XL1-Blue (Stratagene) Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.17 Mg2+
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.17 68000
-
 SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Escherichia coli
-
-
-
2.7.1.17 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.17 harvested cells resuspended in 50 mM Tris/HCl pH 7.8, solution after ultrasonically disruption centrifuged at 10000 *g for 30 min at 4°C, supernatant passed through immobilized metal affinity chromatography (5 ml) using the His-tag introduced into the C-terminal region of XK, purified enzyme dialyzed against 50 mM Tris/Hcl (pH 7.8) containing 12.5% (w/v) glycerol Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.1 0.57
-
-
 Escherichia coli
2.7.1.17 additional information
-
 20.6 +/-0.3 Unit/mg at 25°C, approx. 3 mM D-xylulose 5-phosphate could be produced over 10 min at 25°C from 5 mM xylulose with 0.004 mg purified xylulokinase. With 2.5 mM xylulose, about 80% of conversion occur. Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.1.17 -80°C, 50 mM Tris/HCl, pH 7.8, 12.5% (w/v) glycerol, 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Escherichia coli sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.7.1.17 ATP + D-xylulose
-
 Saccharomyces cerevisiae ADP + D-xylulose 5-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.2.1.1 TKT
-
 Escherichia coli
2.7.1.17 D-xylulokinase
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate
-
 Escherichia coli
2.7.1.17 ATP
-
 Saccharomyces cerevisiae