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Literature summary extracted from

  • Soemphol, W.; Adachi, O.; Matsushita, K.; Toyama, H.
    Distinct physiological roles of two membrane-bound dehydrogenases responsible for D-sorbitol oxidation in Gluconobacter frateurii (2008), Biosci. Biotechnol. Biochem., 72, 842-850.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.21 in Escherichia coli, plasmids harboring the disrupted gene are introduced into Gluconobacter frateurii THD32 by electroporation Gluconobacter frateurii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.22 membrane bound to Gluconobacter frateurii 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.22 additional information Gluconobacter frateurii PQQ-GLDH plays a major role in L-sorbose production ?
-
 ?
1.1.99.22 additional information Gluconobacter frateurii THD32 PQQ-GLDH plays a major role in L-sorbose production ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.21 Gluconobacter frateurii
-
 THD32, wild type and mutant defective in FAD-SLDH
-
1.1.99.22 Gluconobacter frateurii
-
 THD32
-
1.1.99.22 Gluconobacter frateurii THD32
-
 THD32
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.21 by cell disruption and ultracentrifugation Gluconobacter frateurii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.21 D-sorbitol + acceptor production of L-sorbose is similar in the wild-type strain and the FAD-SLDH defective strain, the enzyme is induced by L-sorbose and by D-sorbitol, FAD-SLDH links preferably to the cyanide-insensitive terminal oxidase Gluconobacter frateurii L-sorbose + reduced acceptor
-
 ?
1.1.99.21 D-sorbitol + acceptor production of L-sorbose is similar in the wild-type strain and the FAD-SLDH defective strain, the enzyme is induced by L-sorbose and by D-sorbitol, FAD-SLDH links preferably to the cyanide-insensitive terminal oxidase Gluconobacter frateurii THD32 L-sorbose + reduced acceptor
-
 ?
1.1.99.22 D-sorbitol + acceptor
-
 Gluconobacter frateurii L-sorbose + reduced acceptor
-
 ?
1.1.99.22 D-sorbitol + acceptor
-
 Gluconobacter frateurii THD32 L-sorbose + reduced acceptor
-
 ?
1.1.99.22 additional information PQQ-GLDH plays a major role in L-sorbose production Gluconobacter frateurii ?
-
 ?
1.1.99.22 additional information PQQ-GLDH plays a major role in L-sorbose production Gluconobacter frateurii THD32 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.21 D-sorbitol dehydrogenase membrane-bound FAD-dependent D-sorbitol dehydrogenase FAD-SLDH Gluconobacter frateurii
1.1.99.22 PQQ-GLDH
-
 Gluconobacter frateurii
1.1.99.22 quinone-dependent glycerol dehydrogenase
-
 Gluconobacter frateurii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.21 FAD
-
 Gluconobacter frateurii