Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Munoz-Munoz, J.L.; Garcia-Molina, F.; Varon, R.; Rodriguez-Lopez, J.N.; Garcia-Canovas, F.; Tudela, J.
    Kinetic characterization of the oxidation of esculetin by polyphenol oxidase and peroxidase (2007), Biosci. Biotechnol. Biochem., 71, 390-396.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 0.0052
-
 esculetin at pH 7 Armoracia rusticana
1.11.1.7 0.0052
-
 H2O2 KM as a kinetic constant of the POD/H2O2/ESC system at pH 7 Armoracia rusticana
1.11.1.7 0.052
-
 H2O2 at pH 4.5 Armoracia rusticana
1.11.1.7 0.052
-
 H2O2 KM as a kinetic constant of the POD/H2O2/ESC system at pH 4.5 Armoracia rusticana
1.11.1.7 1.4
-
 esculetin at pH 7 Armoracia rusticana
1.11.1.7 1.4
-
 esculetin KM as a kinetic constant of the POD/H2O2/ESC system at pH 7 Armoracia rusticana
1.11.1.7 2
-
 esculetin at pH 4.5 Armoracia rusticana
1.11.1.7 2
-
 esculetin KM as a kinetic constant of the POD/H2O2/ESC system at pH 4.5 Armoracia rusticana
1.14.18.1 0.04
-
 O2 (KM as a kinetic constant) of the PPO/O2/ESC system at pH 7 Agaricus bisporus
1.14.18.1 0.041
-
 O2 (KM as a kinetic constant) of the PPO/O2/ESC system at pH 4.5 Agaricus bisporus
1.14.18.1 0.63
-
 esculetin (KM as a kinetic constant) of the PPO/O2/ESC system at pH 7 Agaricus bisporus
1.14.18.1 0.9
-
 esculetin (KM as a kinetic constant) of the PPO/O2/ESC system at pH 4.5 Agaricus bisporus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Armoracia rusticana
-
-
-
1.14.18.1 Agaricus bisporus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 by Duckworth and Coleman's procedure, but with two additional chromatographic steps Agaricus bisporus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 commercial preparation
-
 Agaricus bisporus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.7 297
-
-
 Armoracia rusticana
1.14.18.1 1530
-
-
 Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 esculetin + H2O2
-
 Armoracia rusticana ?
-
 ?
1.11.1.7 esculetin + H2O2 demonstration, that esculetin is no inhibitor, but a substrate of mushroom polyphenol oxidase (PPO) and horseradish peroxidase (POD) Armoracia rusticana ?
-
 ?
1.11.1.7 L-3,4-dihydroxyphenylalanine + H2O2
-
 Armoracia rusticana ?
-
 ?
1.11.1.7 L-tyrosine + H2O2
-
 Armoracia rusticana ?
-
 ?
1.14.18.1 esculetin + 1/2 O2 demonstration, that esculetin is no inhibitor, but a substrate of mushroom polyphenol oxidase (PPO) and horseradish peroxidase (POD) Agaricus bisporus 2H-chromene-2,6,7-trione + H2O
-
 ?
1.14.18.1 L-dopa + 1/2 O2
-
 Agaricus bisporus L-dopaquinone + H2O
-
 ?
1.14.18.1 L-tyrosine + O2 + AH2
-
 Agaricus bisporus L-dopa + H2O + A
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 peroxidase
-
 Armoracia rusticana
1.11.1.7 POD
-
 Armoracia rusticana
1.14.18.1 polyphenol oxidase
-
 Agaricus bisporus
1.14.18.1 PPO
-
 Agaricus bisporus
1.14.18.1 tyrosinase
-
 Agaricus bisporus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.7 871
-
 esculetin at pH 7 Armoracia rusticana
1.11.1.7 871
-
 esculetin kcat as a kinetic constant of the POD/H2O2/ESC system at pH 7 Armoracia rusticana
1.11.1.7 890
-
 esculetin at pH 4.5 Armoracia rusticana
1.11.1.7 890
-
 esculetin kcat as a kinetic constant of the POD/H2O2/ESC system at pH 4.5 Armoracia rusticana
1.14.18.1 551
-
 esculetin (kcat as a kinetic constant) of the PPO/O2/ESC system at pH 4.5 Agaricus bisporus
1.14.18.1 928
-
 esculetin (kcat as a kinetic constant) of the PPO/O2/ESC system at pH 7 Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.7 4.5
-
-
 Armoracia rusticana
1.11.1.7 4.5
-
 horsradish peroxidase (POD) Armoracia rusticana
1.14.18.1 7
-
 for polyphenol oxidase (PPO) Agaricus bisporus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.7 4.5 7 assay at 4.5.and 7.0 Armoracia rusticana
1.14.18.1 4.5 7 assay at pH 4.5.and 7.0 Agaricus bisporus