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Literature summary extracted from

  • Takeo, M.; Nishimura, M.; Shirai, M.; Takahashi, H.; Negoro, S.
    Purification and characterization of catechol 2,3-dioxygenase from the aniline degradation pathway of Acinetobacter sp. YAA and its mutant enzyme, which resists substrate inhibition (2007), Biosci. Biotechnol. Biochem., 71, 1668-1675.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.2 E286A mutation results in reduction in activity Acinetobacter sp.
1.13.11.2 E286D activity is similar to wild-type enzyme Acinetobacter sp.
1.13.11.2 E286K specific activity is 2.4fold higher than wild-type activity, in contrast with wild-type enzyme mutant enzyme shows no substrate inhibition Acinetobacter sp.
1.13.11.2 E286R activity is similar to wild-type enzyme Acinetobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.2 catechol wild-type enzyme: substrate inhibition. Mutant enzyme E286K: no substrate inhibition Acinetobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.00147
-
 catechol 25°C, pH 7.2, wild-type enzyme Acinetobacter sp.
1.13.11.2 0.00248
-
 catechol 25°C, pH 7.2, mutant enzyme E286K Acinetobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.2 35000
-
 4 * 35000, SDS-PAGE Acinetobacter sp.
1.13.11.2 147000
-
 gel filtration Acinetobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Acinetobacter sp.
-
 recombinantly expressed in Escherichia coli JM109
-
1.13.11.2 Acinetobacter sp. YAA
-
 recombinantly expressed in Escherichia coli JM109
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2
-
 Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 2,3-dihydroxybiphenyl + O2 5% of the activity with catechol Acinetobacter sp. ?
-
 ?
1.13.11.2 2,3-dihydroxybiphenyl + O2 5% of the activity with catechol Acinetobacter sp. YAA ?
-
 ?
1.13.11.2 3-methylcatechol + O2 19% of the activity with catechol Acinetobacter sp. 2-hydroxy-3-methylmuconate semialdehyde
-
 ?
1.13.11.2 3-methylcatechol + O2 19% of the activity with catechol Acinetobacter sp. YAA 2-hydroxy-3-methylmuconate semialdehyde
-
 ?
1.13.11.2 4-chlorocatechol + O2 46% of the activity with catechol Acinetobacter sp. ?
-
 ?
1.13.11.2 4-chlorocatechol + O2 46% of the activity with catechol Acinetobacter sp. YAA ?
-
 ?
1.13.11.2 4-methylcatechol + O2 57% of the activity with catechol Acinetobacter sp. ?
-
 ?
1.13.11.2 4-methylcatechol + O2 57% of the activity with catechol Acinetobacter sp. YAA ?
-
 ?
1.13.11.2 catechol + O2
-
 Acinetobacter sp. 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Acinetobacter sp. YAA 2-hydroxymuconate semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.2 tetramer 4 * 35000, SDS-PAGE Acinetobacter sp.

Synonyms

EC Number Synonyms Comment Organism
1.13.11.2 AtdB
-
 Acinetobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.2 31.3
-
 catechol 25°C, pH 7.2, wild-type enzyme Acinetobacter sp.
1.13.11.2 34.7
-
 catechol 25°C, pH 7.2, mutant enzyme E286K Acinetobacter sp.