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Literature summary extracted from

  • Shinagawa, E.; Toyama, H.; Matsushita, K.; Tuitemwong, P.; Theeragool, G.; Adachi, O.
    A novel type of formaldehyde-oxidizing enzyme from the membrane of Acetobacter sp. SKU 14 (2006), Biosci. Biotechnol. Biochem., 70, 850-857.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.5.2 additional information
-
 additional information the Km value for formaldehyde is measured to be 10–13 mM using the formaldehyde solution containing methanol. 23–25 mM is obtained with freshly prepared formaldehyde solution only with distilled water. A Km value over 50 mM is observed when using the substrate solution stored in a refrigerator over one month Acetobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.5 membrane
-
 Acetobacter sp. 16020
-
1.2.5.2 membrane
-
 Acetobacter sp. 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.5 18000
-
 x * 78000 + x * 55000 + x * 18000, SDS-PAGE Acetobacter sp.
1.1.5.5 55000
-
 x * 78000 + x * 55000 + x * 18000, SDS-PAGE Acetobacter sp.
1.1.5.5 78000
-
 x * 78000 + x * 55000 + x * 18000, SDS-PAGE Acetobacter sp.
1.2.5.2 18000
-
 1 * 18000 + 1 * 55000 + 1 * 78000, SDS-PAGE Acetobacter sp.
1.2.5.2 18000
-
 x * 78000 + x * 55000 + x * 18000, SDS-PAGE Acetobacter sp.
1.2.5.2 55000
-
 1 * 18000 + 1 * 55000 + 1 * 78000, SDS-PAGE Acetobacter sp.
1.2.5.2 55000
-
 x * 78000 + x * 55000 + x * 18000, SDS-PAGE Acetobacter sp.
1.2.5.2 78000
-
 1 * 18000 + 1 * 55000 + 1 * 78000, SDS-PAGE Acetobacter sp.
1.2.5.2 78000
-
 x * 78000 + x * 55000 + x * 18000, SDS-PAGE Acetobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.5 Acetobacter sp.
-
 isolated in Thailand
-
1.1.5.5 Acetobacter sp. SKU 14
-
 isolated in Thailand
-
1.2.5.2 Acetobacter sp.
-
 SKU 14
-
1.2.5.2 Acetobacter sp.
-
 isolated in Thailand
-
1.2.5.2 Acetobacter sp. SKU 14
-
 SKU 14
-
1.2.5.2 Acetobacter sp. SKU 14
-
 isolated in Thailand
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.5 native enzyme 130fold from membranes of glycerol-grown cells by two different steps of anion exchange chromatography, solubilization with 0.1% Triton X-100 or Tween 20, copurification with a cytochrome c Acetobacter sp.
1.2.5.2
-
 Acetobacter sp.
1.2.5.2 native enzyme 130fold from membranes of glycerol-grown cells by two different steps of anion exchange chromatography, solubilization with 0.1% Triton X-100 or Tween 20, copurification with a cytochrome c Acetobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.5.5 culture condition:glycerol-grown cell
-
 Acetobacter sp.
-
1.2.5.2 culture condition:glycerol-grown cell
-
 Acetobacter sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.5 293
-
 purified native enzyme Acetobacter sp.
1.2.5.2 293
-
 purified native enzyme Acetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.5 ethanol + ferricyanide about 40% of the activity with n-butanol Acetobacter sp. acetaldehyde + ferrocyanide
-
 ?
1.1.5.5 iso-propanol + ferricyanide about 10% of the activity with n-butanol Acetobacter sp. propan-2-one + ferrocyanide
-
 ?
1.1.5.5 n-butanol + ferricyanide
-
 Acetobacter sp. n-butanal + ferrocyanide
-
 ?
1.1.5.5 n-pentanol + ferricyanide about 45% of the activity with n-butanol Acetobacter sp. n-pentanal + ferrocyanide
-
 ?
1.1.5.5 n-propanol + ferricyanide about 95% of the activity with n-butanol Acetobacter sp. n-propanal + ferrocyanide
-
 ?
1.2.5.2 acetaldehyde + 2 ferricyanide + H2O
-
 Acetobacter sp. acetate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 acetaldehyde + 2 ferricyanide + H2O about 30% of the activity with formaldehyde Acetobacter sp. acetate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 acetaldehyde + 2 ferricyanide + H2O
-
 Acetobacter sp. SKU 14 acetate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 acetaldehyde + 2 ferricyanide + H2O about 30% of the activity with formaldehyde Acetobacter sp. SKU 14 acetate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 butanal + 2 ferricyanide + H2O
-
 Acetobacter sp. butanoate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 butanal + 2 ferricyanide + H2O about 30% of the activity with formaldehyde Acetobacter sp. butanoate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 butanal + 2 ferricyanide + H2O
-
 Acetobacter sp. SKU 14 butanoate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 butanal + 2 ferricyanide + H2O about 30% of the activity with formaldehyde Acetobacter sp. SKU 14 butanoate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 formaldehyde + 2 ferricyanide + H2O
-
 Acetobacter sp. formate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 glutaraldehyde + 4 ferricyanide + 2 H2O
-
 Acetobacter sp. glutarate + 4 ferrocyanide + 4 H+
-
 ?
1.2.5.2 glutaraldehyde + 4 ferricyanide + 2 H2O about 40% of the activity with formaldehyde Acetobacter sp. glutarate + 4 ferrocyanide + 4 H+
-
 ?
1.2.5.2 additional information the substrate specificity of the enzyme is broad toward aldehydes and alcohols Acetobacter sp. ?
-
 ?
1.2.5.2 additional information the substrate specificity of the enzyme is broad toward aldehydes and alcohols Acetobacter sp. SKU 14 ?
-
 ?
1.2.5.2 propanal + 2 ferricyanide + H2O
-
 Acetobacter sp. propanoate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 propanal + 2 ferricyanide + H2O about 40% of the activity with formaldehyde Acetobacter sp. propanoate + 2 ferrocyanide + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.5 ? x * 78000 + x * 55000 + x * 18000, SDS-PAGE Acetobacter sp.
1.2.5.2 ? x * 78000 + x * 55000 + x * 18000, SDS-PAGE Acetobacter sp.
1.2.5.2 trimer 1 * 18000 + 1 * 55000 + 1 * 78000, SDS-PAGE Acetobacter sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.5.5 formaldehyde-oxidizing enzyme
-
 Acetobacter sp.
1.2.5.2 FOE
-
 Acetobacter sp.
1.2.5.2 formaldehyde-oxidizing enzyme
-
 Acetobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.5 40
-
-
 Acetobacter sp.
1.2.5.2 40
-
-
 Acetobacter sp.
1.2.5.2 40
-
 substrate: formaldehyde Acetobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.5.5 25 50 25°C: about 75% of maximal activity, 50°C: about 60% of maximal activity Acetobacter sp.
1.2.5.2 25 50 25°C: about 75% of maximal activity, 50°C: about 60% of maximal activity Acetobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.5 40
-
 10 min, stable Acetobacter sp.
1.1.5.5 45
-
 10 min, about 50% loss of activity Acetobacter sp.
1.1.5.5 50
-
 10 min, 95% loss of activity Acetobacter sp.
1.2.5.2 40
-
 10 min, stable Acetobacter sp.
1.2.5.2 45
-
 10 min, about 50% loss of activity Acetobacter sp.
1.2.5.2 50
-
 10 min, 95% loss of activity Acetobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.5 5 6
-
 Acetobacter sp.
1.2.5.2 5 6
-
 Acetobacter sp.
1.2.5.2 5 6 substrate: formaldehyde Acetobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.5.2 5 9 pH 5-6: optimum, pH 9: about 60% of maximal activity Acetobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.5.5 3 9 stable at 6°C, overnight Acetobacter sp.
1.1.5.5 10
-
 at 6°C, overnight, about 75% inactivation Acetobacter sp.
1.1.5.5 11
-
 at 6°C, overnight, complete inactivation Acetobacter sp.
1.2.5.2 3
-
 overnight, at 6°C, about 20% loss of activity Acetobacter sp.
1.2.5.2 3 9 stable at 6°C, overnight Acetobacter sp.
1.2.5.2 5 7 overnight, at 6°C, stable Acetobacter sp.
1.2.5.2 9
-
 overnight, at 6°C, about 20% loss of activity Acetobacter sp.
1.2.5.2 10
-
 at 6°C, overnight, about 75% inactivation Acetobacter sp.
1.2.5.2 10
-
 overnight, at 6°C, about 60% loss of activity Acetobacter sp.
1.2.5.2 11
-
 at 6°C, overnight, complete inactivation Acetobacter sp.
1.2.5.2 11
-
 overnight, at 6°C, complete loss of activity Acetobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.5 heme the two subunits of 78000 Da and 55000 Da contain cytochrome c Acetobacter sp.
1.1.5.5 pyrroloquinoline quinone
-
 Acetobacter sp.
1.2.5.2 heme the two subunits of 78000 Da and 55000 Da contain cytochrome c Acetobacter sp.
1.2.5.2 pyrroloquinoline quinone
-
 Acetobacter sp.