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Literature summary extracted from

  • Song, Z.; Nes, W.D.
    Inactivation of soybean sterol 24-C-methyltransferase by elongated sterol side chains at C26 (2007), Bioorg. Med. Chem. Lett., 17, 5902-5906.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.142 expressed in Escherichia coli, recombinant protein Glycine max
2.1.1.142 expression in Escherichia coli Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.142 24,25-dehydro-27-methenyl-cycloartenol i.e. cycloartenol nucleus substrate analogue with double bond at C8 Glycine max
2.1.1.142 24,25-dehydro-27-methylene-cycloartenol i.e. cycloartenol nucleus substrate analogue with double bond at C14, non-competitive Glycine max
2.1.1.142 24-dehydrocycloartenol a mechanism-based inactivator Glycine max
2.1.1.142 24-thiacycloartanol a mechanism-based inactivator Glycine max
2.1.1.142 26-methenylcycloartenol pseudo-first-order time-dependent inactivation, less potency of inhibition Glycine max
2.1.1.142 26-methynylcycloartenol inhibition kinetics non-competitive and time-dependent, less potency of inhibition Glycine max
2.1.1.142 Cycloartenol
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.142 additional information
-
 additional information kinetics, overview Glycine max
2.1.1.142 0.005
-
 24,25-dehydro-27-methylene-cycloartenol pH 7.5, 35°C, recombinant enzyme Glycine max
2.1.1.142 0.03
-
 Cycloartenol pH 7.5, 35°C, recombinant enzyme Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.142 Mg2+
-
 Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.142 cycloartenol + S-adenosyl-L-methionine Glycine max
-
 (24S)-24-methylcycloart-25-en-3beta-ol + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.142 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.142 partially purified, recombinant protein, SDS-PAGE Glycine max
2.1.1.142 recombinant enzyme from Escherichia coli to homogeneity Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.142 culture supernatant
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.142 additional information
-
 C-methylation reaction analyzed with substrate analogs containing a cycloartenol nucleus and a double bond or triple bond attached to C26, mass spectra of enzyme-generated products indicated, time-dependent inhibition kinetics reveals active-site directed process and partitioning to produce novel products Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.142 24,25-dehydro-27-methenyl-cycloartenol + S-adenosyl-L-methionine CA-8, i.e. cycloartenol nucleus substrate analogue with double bond at C8, an enzyme inhibitor, is a poor substrate Glycine max ?
-
 ?
2.1.1.142 24,25-dehydro-27-methylene-cycloartenol + S-adenosyl-L-methionine CA-14, i.e. cycloartenol nucleus substrate analogue with double bond at C14, an enzyme inhibitor, is a poor substrate Glycine max ?
-
 ?
2.1.1.142 cycloartenol + S-adenosyl-L-methionine
-
 Glycine max (24S)-24-methylcycloart-25-en-3beta-ol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 additional information the SMT from soybean plants is bifunctional, performing methyl transferations to C24 and to C28 of the substrate, the enzyme also converts the inhibitor 24,25-dehydro-27-methenyl-cycloartenol, i.e. cycloartenol nucleus substrate analogue with double bond at C8, to a 24,27-dimethenyl-cycloartenol, CA-11, compound, overview Glycine max ?
-
 ?
2.1.1.142 S-adenosyl-L-methionine + cycloartenol active-site topography and reaction mechanisms analyzed with substrate analogs Glycine max S-adenosyl-L-homocysteine + 24-methylenecycloartanol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.142 More the enzyme belongs to a family of sterol 24-C-methyltransferases that perform distinct C1-activities, two different alkylations at C24 or C28, alkylation of the latter affects membrane fluidity Glycine max
2.1.1.142 SMT
-
 Glycine max
2.1.1.142 SMT protein
-
 Glycine max
2.1.1.142 sterol 24-C methyltransferase
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.142 35
-
 assay at Glycine max
2.1.1.142 35
-
 inhibition assay at Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.142 0.00000083
-
 24,25-dehydro-27-methylene-cycloartenol pH 7.5, 35°C, recombinant enzyme Glycine max
2.1.1.142 0.01
-
 Cycloartenol pH 7.5, 35°C, recombinant enzyme Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.142 7.5
-
 assay at Glycine max
2.1.1.142 7.5
-
 inhibition assay at Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.142 S-adenosyl-L-methionine
-
 Glycine max

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.142 additional information
-
 additional information inhibition kinetics, overview Glycine max
2.1.1.142 0.002
-
 24-thiacycloartanol pH 7.5, 35°C, recombinant enzyme Glycine max
2.1.1.142 0.03
-
 Cycloartenol natural substrate Glycine max
2.1.1.142 0.042
-
 24-dehydrocycloartenol pH 7.5, 35°C, recombinant enzyme Glycine max
2.1.1.142 0.047
-
 24,25-dehydro-27-methenyl-cycloartenol pH 7.5, 35°C, recombinant enzyme Glycine max
2.1.1.142 0.047
-
 26-methenylcycloartenol rate of inactivation saturable, comparable to values obtained for cycloartenol Glycine max
2.1.1.142 0.067
-
 24,25-dehydro-27-methylene-cycloartenol pH 7.5, 35°C, recombinant enzyme Glycine max

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.142 0.067
-
 co-incubation with 50 microM or 100 microM cycloartenol affords protection against inactivation generating 25% and 45% C-methylation Glycine max 26-methynylcycloartenol