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Literature summary extracted from

  • Revell, K.D.; Heldreth, B.; Long, T.E.; Jang, S.; Turos, E.
    N-Thiolated beta-lactams: Studies on the mode of action and identification of a primary cellular target in Staphylococcus aureus (2007), Bioorg. Med. Chem., 15, 2453-2467.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.14 0.004
-
 CoA-sec-butyl disulfide 25°C, pH 7.8 Staphylococcus aureus
1.8.1.14 0.005
-
 CoA-ethyl disulfide 25°C, pH 7.8 Staphylococcus aureus
1.8.1.14 0.006
-
 CoA-disulfide 25°C, pH 7.8 Staphylococcus aureus
1.8.1.14 0.008
-
 CoA-methyl disulfide 25°C, pH 7.8 Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.14 Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.14 CoA-disulfide + NADPH + H+ reduction of CoA disulfide occurs through two steps: thiol-disulfide exchange with the active site cysteine, followed by flavin-mediated hydride transfer from NADPH to reduce the cysteine-CoA disulfide bond and regenerate the active site Staphylococcus aureus CoA + NADP+
-
 ?
1.8.1.14 CoA-ethyl disulfide + NADPH + H+
-
 Staphylococcus aureus ?
-
 ?
1.8.1.14 CoA-methyl disulfide + NADPH + H+
-
 Staphylococcus aureus ?
-
 ?
1.8.1.14 CoA-sec-butyl disulfide + NADPH + H+
-
 Staphylococcus aureus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.14 CoA disulfide reductase
-
 Staphylococcus aureus
1.8.1.14 coenzyme A disulfide reductase
-
 Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.14 0.95
-
 CoA-sec-butyl disulfide 25°C, pH 7.8 Staphylococcus aureus
1.8.1.14 2.7
-
 CoA-ethyl disulfide 25°C, pH 7.8 Staphylococcus aureus
1.8.1.14 3.17
-
 CoA-disulfide 25°C, pH 7.8 Staphylococcus aureus
1.8.1.14 4.3
-
 CoA-methyl disulfide 25°C, pH 7.8 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.14 NADPH
-
 Staphylococcus aureus