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Literature summary extracted from

  • Hyun, S.K.; Lee, W.H.; Jeong, d.a..M.; Kim, Y.; Choi, J.S.
    Inhibitory effects of kurarinol, kuraridinol, and trifolirhizin from Sophora flavescens on tyrosinase and melanin synthesis (2008), Biol. Pharm. Bull., 31, 154-158.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.18.1 pharmacology kurarinol, kuraridinol, and trifolirhizin are candidates as skin-whitening agents Agaricus bisporus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 kojic acid inhibitory effects with kojic acid Agaricus bisporus
1.14.18.1 kuraridinol prenylated flavonoid from Sophora flavescens, isolated from the EtOAc fraction, inhibitory effects on tyrosinase and melanin synthesis. Inhibitory activity 20times more potent than that of the positive control, kojic acid. Kuraridinol is a chalcone compound belonging to the prenylated flavonoids Agaricus bisporus
1.14.18.1 kurarinol prenylated flavonoid from Sophora flavescens, isolated from the EtOAc fraction, inhibitory effects on tyrosinase and melanin synthesis. Kurarinol is a prenylated flavanone and an effective inhibitor of alpha-glucosidase, beta-amylase, and cGMP phosphodiesterase-5, and also inhibits diacylglycerol acyltransferase activity Agaricus bisporus
1.14.18.1 additional information compounds from Sophora flavescens are further tested for their inhibitory effects on melanogenesis in B16 melanoma cells. MeOH extract fraction, CH2Cl2 fraction, EtOAc fraction and, n-BuOH fraction from Sophora flavescens inhibit L-tyrosine oxidation catalyzed by tyrosinase in concentration dependent manner. EtOAc fraction has more potent inhibitory activity than the other fractions Agaricus bisporus
1.14.18.1 trifolirhizin prenylated flavonoid from Sophora flavescens, isolated from the EtOAc fraction, inhibitory effects on tyrosinase and melanin synthesis Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 2.4
-
 L-tyrosine in the presence of kuraridinol Agaricus bisporus
1.14.18.1 2.9
-
 L-tyrosine in the presence of kurarinol Agaricus bisporus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Agaricus bisporus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 commercial preparation
-
 Agaricus bisporus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 additional information
-
 for tyrosinase inhibitory activity assay mushroom tyrosinase (1000 U/ml) used Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 L-tyrosine + O2 + AH2
-
 Agaricus bisporus L-dopa + H2O + A
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 polyphenol oxidase
-
 Agaricus bisporus
1.14.18.1 PPO
-
 Agaricus bisporus
1.14.18.1 tyrosinase
-
 Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 6.5
-
 assay at Agaricus bisporus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.18.1 0.00039
-
 kuraridinol noncompetitive inhibition Agaricus bisporus
1.14.18.1 0.014
-
 kurarinol noncompetitive inhibition Agaricus bisporus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.18.1 0.88
-
 potent tyrosinase inhibitory activity Agaricus bisporus kuraridinol
1.14.18.1 8.6
-
 potent tyrosinase inhibitory activity Agaricus bisporus kurarinol
1.14.18.1 506.8
-
 moderate inhibitory activity Agaricus bisporus trifolirhizin