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Literature summary extracted from

  • Zhou, L.; Poh, R.P.; Marks, T.S.; Chowdhry, B.Z.; Smith, A.R.
    Structure and denaturation of 4-chlorobenzoyl coenzyme A dehalogenase from Arthrobacter sp. strain TM-1 (2008), Biodegradation, 19, 65-75.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.7 4-chlorobenzoyl-CoA + H2O Arthrobacter sp.
-
 4-hydroxybenzoyl-CoA + chloride
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.7 Arthrobacter sp.
-
 Arthrobacter sp. strain TM-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.7 4-CBCoA dehalogenase is purified to homogeneity from cells of Arthrobacter sp. strain TM-1 Arthrobacter sp.

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.8.1.7 denaturation and renaturation experiments as functions of pH and temperature are performed Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.7 0.007
-
 time elapsed at 45°C, 1020 min, recovery 1.7% Arthrobacter sp.
3.8.1.7 0.08
-
 time elapsed at 45°C, 120 min, recovery 19.9% Arthrobacter sp.
3.8.1.7 0.082
-
 time elapsed at 45°C, 0 min, recovery 20.4% Arthrobacter sp.
3.8.1.7 0.082
-
 time elapsed at 45°C, 15 min, recovery 20.4% Arthrobacter sp.
3.8.1.7 0.082
-
 time elapsed at 45°C, 5 min, recovery 20.4% Arthrobacter sp.
3.8.1.7 0.194
-
 renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 55°C, activity after heating to 60°C 0.081, recovery 20.1% Arthrobacter sp.
3.8.1.7 0.243
-
 renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 20°C, activity after heating to 60°C 0.038, recovery 9.5% Arthrobacter sp.
3.8.1.7 0.301
-
 renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 30°C, activity after heating to 60°C 0.051, recovery 12.7% Arthrobacter sp.
3.8.1.7 0.349
-
 renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 50°C, activity after heating to 60°C 0.082, recovery 20.4% Arthrobacter sp.
3.8.1.7 0.36
-
 renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 40°C, activity after heating to 60°C 0.055, recovery 13.7% Arthrobacter sp.
3.8.1.7 0.363
-
 renaturation of 4-CBCoA dehalogenaase after return from pH 5.2 to pH 7.4, incubation time 7 min, recovery 89% Arthrobacter sp.
3.8.1.7 0.365
-
 renaturation of 4-CBCoA dehalogenaase after return from pH 10.1 to pH 7.4, incubation time 7 min, recovery 90% Arthrobacter sp.
3.8.1.7 0.402
-
 renaturation of 4-CBCoA dehalogenaase following exposure to elevated temperature, 45°C, activity after heating to 60°C 0.083, recovery 20.6% Arthrobacter sp.
3.8.1.7 0.407
-
 renaturation of 4-CBCoA dehalogenaase after return from pH 10.1 to pH 7.4, incubation time 17 min, recovery 99% Arthrobacter sp.
3.8.1.7 0.41
-
 pH 7.4 Arthrobacter sp.
3.8.1.7 0.41
-
 renaturation of 4-CBCoA dehalogenaase after return from pH 5.2 to pH 7.4, incubation time 17 min, recovery 100% Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.7 4-chlorobenzoyl-CoA + H2O
-
 Arthrobacter sp. 4-hydroxybenzoyl-CoA + chloride
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.7 trimer
-
 Arthrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.8.1.7 4-CBCoA dehalogenase
-
 Arthrobacter sp.
3.8.1.7 4-chlorobenzoyl-CoA dehalogenase
-
 Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.7 30
-
 activity assay Arthrobacter sp.
3.8.1.7 45
-
 activity is maximal at 45°C and is totally lost by heating to 60°C for 5 min Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.7 7.4
-
 activity assay Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.8.1.7 7.4
-
 when incubated for 5 Min at 1.5 mg ml-1 in buffer at 5.2 or 10.1 enzyme activity is lost completely Arthrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
3.8.1.7 coenzyme A
-
 Arthrobacter sp.