EC Number | Application | Comment | Organism |
---|---|---|---|
3.8.1.5 | environmental protection | organisms producing this enzyme are of great interest as bioremediants, the enzyme has also been shown to have uses in combating chemical warfare where it can act against toxic agents like mustard gas | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.8.1.5 | the ORF of Rv2579 is inserted into the cloning vector pDONR-201, the resulting vector is used to transfer the coding sequence into the expression vector pDET-15 | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.8.1.5 | the crystal structure of Rv2579 is determined at a resolution of 1.2 A, in addition, the structures in complex with 1,3-propanediol and 1,2-dichloroethane are determined | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.8.1.5 | 1-haloalkane + H2O | Mycobacterium tuberculosis | - |
a primary alcohol + halide | - |
? | |
3.8.1.5 | 1-haloalkane + H2O | Mycobacterium tuberculosis H37Rv | - |
a primary alcohol + halide | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.8.1.5 | Mycobacterium tuberculosis | P9WMR9 | - |
- |
3.8.1.5 | Mycobacterium tuberculosis H37Rv | P9WMR9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.8.1.5 | using a glutathione sepharose column and size-exclusion chromatography, the GST tag is removed by digestion with AcTEV protease | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.8.1.5 | 1,2-dichloroethane + 2 H2O | - |
Mycobacterium tuberculosis | 1,2-ethanediol + 2 chloride | - |
? | |
3.8.1.5 | 1,2-dichloroethane + 2 H2O | - |
Mycobacterium tuberculosis H37Rv | 1,2-ethanediol + 2 chloride | - |
? | |
3.8.1.5 | 1,3-dibromopropane + 2 H2O | - |
Mycobacterium tuberculosis | 1,3-propanediol + 2 bromide | - |
? | |
3.8.1.5 | 1,3-dibromopropane + 2 H2O | - |
Mycobacterium tuberculosis H37Rv | 1,3-propanediol + 2 bromide | - |
? | |
3.8.1.5 | 1-haloalkane + H2O | - |
Mycobacterium tuberculosis | a primary alcohol + halide | - |
? | |
3.8.1.5 | 1-haloalkane + H2O | - |
Mycobacterium tuberculosis H37Rv | a primary alcohol + halide | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.8.1.5 | haloalkane dehalogenase | - |
Mycobacterium tuberculosis |
3.8.1.5 | Rv2579 | - |
Mycobacterium tuberculosis |