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Literature summary extracted from

  • Fauvart, M.; Braeken, K.; Daniels, R.; Vos, K.; Ndayizeye, M.; Noben, J.; Robben, J.; Vanderleyden, J.; Michiels, J.
    Identification of a novel glyoxylate reductase supports phylogeny-based enzymatic substrate specificity prediction (2007), Biochim. Biophys. Acta, 1774, 1092-1098.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.26 gene gxrA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21 Rhizobium etli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.26 0.8
-
phenylpyruvate pH 7.0, 37°C Rhizobium etli
1.1.1.26 2.5
-
glyoxylate pH 7.0, 37°C Rhizobium etli
1.1.1.26 6.8
-
Hydroxypyruvate pH 7.0, 37°C Rhizobium etli
1.1.1.26 40
-
2-oxobutyrate pH 7.0, 37°C Rhizobium etli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.26 37000
-
2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry Rhizobium etli
1.1.1.26 38420
-
2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry Rhizobium etli
1.1.1.26 77200
-
recombinant His-tagged enzyme, gel filtration Rhizobium etli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.26 glyoxylate + NADH Rhizobium etli GxrA glycolate + NAD+
-
ir
1.1.1.26 glyoxylate + NADH Rhizobium etli CFN 42 GxrA glycolate + NAD+
-
ir
1.1.1.26 additional information Rhizobium etli in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview ?
-
?
1.1.1.26 additional information Rhizobium etli CFN 42 in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview ?
-
?
1.1.1.26 phenylpyruvate + NADH Rhizobium etli GxrA phenyllactate + NAD+
-
ir
1.1.1.26 phenylpyruvate + NADH Rhizobium etli CFN 42 GxrA phenyllactate + NAD+
-
ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.26 Rhizobium etli
-
strain CFN42, gene gxrA
-
1.1.1.26 Rhizobium etli CFN 42
-
strain CFN42, gene gxrA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.26 recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration Rhizobium etli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.26 2-oxobutyrate + NADH GxrA Rhizobium etli isovalerate + NAD+
-
?
1.1.1.26 glyoxylate + NADH GxrA Rhizobium etli glycolate + NAD+
-
ir
1.1.1.26 glyoxylate + NADH GxrA Rhizobium etli CFN 42 glycolate + NAD+
-
ir
1.1.1.26 hydroxypyruvate + NADH GxrA Rhizobium etli D-glycerate + NAD+
-
?
1.1.1.26 hydroxypyruvate + NADH GxrA Rhizobium etli CFN 42 D-glycerate + NAD+
-
?
1.1.1.26 additional information in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview Rhizobium etli ?
-
?
1.1.1.26 additional information no activity of GxrA with 2-oxoglutarate, 2-oxo-D-gluconate, and indole in the reductive direction, as well as with glycolate, D-phenyllactate, and D-gluconate in the oxidative direction Rhizobium etli ?
-
?
1.1.1.26 additional information in the endosymbiont, a 3-phosphoglycerate dehydrogenase exhibits glyoxylate/hydroxypyruvate reductase activity, substrate specificity, overview Rhizobium etli CFN 42 ?
-
?
1.1.1.26 additional information no activity of GxrA with 2-oxoglutarate, 2-oxo-D-gluconate, and indole in the reductive direction, as well as with glycolate, D-phenyllactate, and D-gluconate in the oxidative direction Rhizobium etli CFN 42 ?
-
?
1.1.1.26 phenylpyruvate + NADH GxrA Rhizobium etli phenyllactate + NAD+
-
ir
1.1.1.26 phenylpyruvate + NADH GxrA Rhizobium etli CFN 42 phenyllactate + NAD+
-
ir

Subunits

EC Number Subunits Comment Organism
1.1.1.26 dimer 2 * 37000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 38420, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry Rhizobium etli

Synonyms

EC Number Synonyms Comment Organism
1.1.1.26 GHPR
-
Rhizobium etli
1.1.1.26 glyoxylate/hydroxypyruvate reductase
-
Rhizobium etli
1.1.1.26 GxrA a 3-phosphoglycerate dehydrogenase exhibiting glyoxylate/hydroxypyruvate reductase activity Rhizobium etli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.26 37
-
assay at Rhizobium etli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.26 0.25
-
Hydroxypyruvate pH 7.0, 37°C Rhizobium etli
1.1.1.26 0.37
-
phenylpyruvate pH 7.0, 37°C Rhizobium etli
1.1.1.26 0.5
-
2-oxobutyrate pH 7.0, 37°C Rhizobium etli
1.1.1.26 1.35
-
glyoxylate pH 7.0, 37°C Rhizobium etli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.26 7
-
with substrate phenylpyruvate Rhizobium etli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.26 5.5 9.5
-
Rhizobium etli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.26 NADH is not replacable by NADPH with GxrA Rhizobium etli