EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.3.21 | expressed in Escherichia coli, strain B834, pQE30 and pREP4 vectors | Streptococcus sp. |
1.1.5.3 | deletion mutant of Streptococcus sp. GlpO (GlpODELTA, lacking a 50-residue insert that includes a flexible surface region) is expressed in Escherichia coli | Streptococcus sp. |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.3.21 | intact GlpO structure, refined at 2.4 A resolution and structure of a deletion mutant lacking 50-residue insert, refined at 2.3 A resolution, multiwavelength anomalous dispersion data | Streptococcus sp. |
1.1.5.3 | structure of a deletion mutant of Streptococcus sp. GlpO (GlpODELTA, lacking a 50-residue insert that includes a flexible surface region) is determined using multiwavelength anomalous dispersion data and refined at 2.3 A resolution | Streptococcus sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.3.21 | additional information | deletion mutant, 50-residue insert lacked consisting of residues Asp356-Ala405 including a flexible surface region | Streptococcus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.21 | carboxylate | structural characterization of enzyme-inhibitor interaction | Streptococcus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.21 | 0.052 | - |
O2 | pH 7.0, 5°C, wild-type enzyme, intact | Streptococcus sp. | |
1.1.3.21 | 0.069 | - |
O2 | pH 7.0, 5°C, wild-type enzyme, nicked | Streptococcus sp. | |
1.1.3.21 | 0.46 | - |
O2 | pH 7.0, 5°C, recombinant deletion mutant | Streptococcus sp. | |
1.1.3.21 | 1.9 | - |
sn-glycerol 3-phosphate | pH 7.0, 5°C, wild-type enzyme, intact | Streptococcus sp. | |
1.1.3.21 | 1.9 | - |
sn-glycerol 3-phosphate | intact enzyme form, at pH 7.0 and 25°C | Streptococcus sp. | |
1.1.3.21 | 6.6 | - |
sn-glycerol 3-phosphate | pH 7.0, 5°C, recombinant deletion mutant | Streptococcus sp. | |
1.1.3.21 | 6.6 | - |
sn-glycerol 3-phosphate | deletion mutant GlpODELTA, at pH 7.0 and 25°C | Streptococcus sp. | |
1.1.3.21 | 36.2 | - |
sn-glycerol 3-phosphate | pH 7.0, 5°C, wild-type enzyme, nicked | Streptococcus sp. | |
1.1.3.21 | 36.2 | - |
sn-glycerol 3-phosphate | nicked enzyme form, at pH 7.0 and 25°C | Streptococcus sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.21 | sn-glycerol-3-phosphate + O2 | Streptococcus sp. | structural characterization, substrate binding site | glycerone phosphate + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.21 | Streptococcus sp. | - |
- |
- |
1.1.5.3 | Streptococcus sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.21 | gel filtration, recombinant protein | Streptococcus sp. |
1.1.5.3 | deletion mutant of Streptococcus sp. GlpO (GlpODELTA, lacking a 50-residue insert that includes a flexible surface region) | Streptococcus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.21 | additional information | - |
structural characterization, wild-type and deletion mutant, FAD- and substrate binding sites identified, active-site overlays, residues interacting with the amino acid substrate or with inhibitor carboxylate identified, structural and functional divergence between alpha-glycerophosphate oxidase (GlpO) and the bacterial and mitochondrial alpha-glycerophosphate dehydrogenases (GlpDs) discussed | Streptococcus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.21 | sn-glycerol 3-phosphate + O2 | - |
Streptococcus sp. | glycerone phosphate + H2O2 | - |
r | |
1.1.3.21 | sn-glycerol-3-phosphate + O2 | structural characterization, substrate binding site | Streptococcus sp. | glycerone phosphate + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.3.21 | GlpO protein | - |
Streptococcus sp. |
1.1.5.3 | GlpO | - |
Streptococcus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.21 | 11.2 | - |
sn-glycerol 3-phosphate | 10 microM enzyme and 0.77 mM O2, substrate concentration ranges between 2.5 and 50 mM, pH 7.0, 5°C, recombinant deletion mutant | Streptococcus sp. | |
1.1.3.21 | 14.1 | - |
sn-glycerol 3-phosphate | 10 microM enzyme and 0.77 mM O2, substrate concentration ranges between 2.5 and 50 mM, pH 7.0, 5°C, wild-type enzyme, nicked | Streptococcus sp. | |
1.1.3.21 | 17.9 | - |
sn-glycerol 3-phosphate | 10 microM enzyme and 0.77 mM O2, substrate concentration ranges between 2.5 and 50 mM, pH 7.0, 5°C, wild-type enzyme, intact | Streptococcus sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.21 | FAD | the first two domains of the GlpO protein fold involved in FAD binding, linkage of substrate-binding domain to a beta-beta-alpha element of the FAD-binding domain | Streptococcus sp. | |
1.1.5.3 | FAD | dependent on | Streptococcus sp. |