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Literature summary extracted from

  • Jiang, W.; Xie, J.; N?rgaard, H.; Bollinger, J.M.; Krebs, C.
    Rapid and quantitative activation of Chlamydia trachomatis ribonucleotide reductase by hydrogen peroxide (2008), Biochemistry, 47, 4477-4483.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.1 ATP
-
 Chlamydia trachomatis
1.17.4.1 DTT
-
 Chlamydia trachomatis
1.17.4.1 additional information quantitative activation Chlamydia trachomatis

Application

EC Number Application Comment Organism
1.17.4.1 pharmacology inhibition of RNRs is a proven strategy for combating cancer and some viruses Chlamydia trachomatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.1 additional information
-
 additional information kinetics, overview Chlamydia trachomatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.1 CDP + thioredoxin Chlamydia trachomatis
-
 2'-deoxyCDP + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.1 Chlamydia trachomatis
-
 a human pathogen
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.17.4.1 the enzyme is not stable to to reactive oxygen and nitrogen species, RO(N)S, produced by the host’s immune system Chlamydia trachomatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.1 CDP + thioredoxin
-
 Chlamydia trachomatis 2'-deoxyCDP + thioredoxin disulfide + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.4.1 tetramer 2 * R1 subunit + 2 * R2 subunit Chlamydia trachomatis

Synonyms

EC Number Synonyms Comment Organism
1.17.4.1 More a class Ic ribonucleotide reductase Chlamydia trachomatis
1.17.4.1 RNR
-
 Chlamydia trachomatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.1 22
-
 assay at Chlamydia trachomatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.1 manganese-iron cofactor the class Ic RNR from Chlamydia trachomatis uses a Mn(IV)/Fe(III) cofactor, with high specificity for MnIV, which functionally replaces the tyrosyl radical used by conventional class I RNRs to initiate substrate radical production. The intermediate decays by reduction of the Fe site to the active MnIV/FeIII-R2 complex. The reaction of the MnII/FeII-R2 species with H2O2 proceeds in three resolved steps: sequential oxidation to MnIII/FeIII-R2 and Mn(IV)/Fe(IV)-R2, followed by decay of the intermediate to the active Mn(IV)/Fe(III)-R2 product, kinetics and reaction mechanism, overview Chlamydia trachomatis
1.17.4.1 thioredoxin
-
 Chlamydia trachomatis