Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Xu, H.; Sullivan, T.J.; Sekiguchi, J.; Kirikae, T.; Ojima, I.; Stratton, C.F.; Mao, W.; Rock, F.L.; Alley, M.R.; Johnson, F.; Walker, S.G.; Tonge, P.J.
    Mechanism and inhibition of saFabI, the enoyl reductase from Staphylococcus aureus (2008), Biochemistry, 47, 4228-4236.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.10 expressed in Escherichia coli BL21(DE3)pLysS cells Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.10 A95V the mutant correlates with resistance to diphenyl ethers and causes a significant reduction in the affinity of the inhibitors for the enzyme Staphylococcus aureus
1.3.1.10 F204S inactive, the mutant correlates with resistance to diphenyl ethers and causes a significant reduction in the affinity of the inhibitors for the enzyme Staphylococcus aureus
1.3.1.10 I193S the mutant correlates with resistance to diphenyl ethers and causes a significant reduction in the affinity of the inhibitors for the enzyme Staphylococcus aureus
1.3.1.10 K41N strongly decreased activity for NADPH and increased activity with NADH Staphylococcus aureus
1.3.1.10 R40Q strongly decreased activity for NADPH and increased activity with NADH Staphylococcus aureus
1.3.1.10 R40Q/K41N strongly decreased activity for NADPH and increased activity with NADH Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.10 5-chloro-2-phenoxyphenol
-
 Staphylococcus aureus
1.3.1.10 5-ethyl-2-phenoxyphenol
-
 Staphylococcus aureus
1.3.1.10 triclosan
-
 Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.10 0.0045
-
 dodecenoyl-[Staphylococcus aureus acyl carrier protein] wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 0.008
-
 Crotonyl-N-acetyl-cysteamine wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 0.0114
-
 dodecenoyl-[Staphylococcus aureus acyl carrier protein] mutant enzyme A95V, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 0.0115
-
 crotonyl-[Staphylococcus aureus acyl carrier protein] wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 0.0184
-
 dodecenoyl-[Staphylococcus aureus acyl carrier protein] mutant enzyme F204S, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 0.0233
-
 dodecenoyl-N-acetyl-cysteamine wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 0.0241
-
 dodec-2-enoyl-CoA wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 0.2694
-
 NADPH mutant enzyme A95V, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 0.4296
-
 NADPH mutant enzyme F204S, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 1
-
 NADPH Km above 1 mM, mutant enzyme I193S, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.10 30000
-
 SDS-PAGE Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.10 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.10 Ni2+-NTA resin chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.10 crotonyl-N-acetyl-cysteamine + NADPH + H+
-
 Staphylococcus aureus butyryl-N-acetyl-cysteamine + NADP+
-
 ?
1.3.1.10 crotonyl-[Staphylococcus aureus acyl carrier protein] + NADPH + H+
-
 Staphylococcus aureus butyryl-[Staphylococcus aureus acyl carrier protein] + NADP+
-
 ?
1.3.1.10 dodec-2-enoyl-CoA + NADPH + H+
-
 Staphylococcus aureus dodecanoyl-CoA + NADP+
-
 r
1.3.1.10 dodecenoyl-CoA + NADPH + H+
-
 Staphylococcus aureus dodecanoyl-CoA + NADP+
-
 ?
1.3.1.10 dodecenoyl-N-acetyl-cysteamine + NADPH + H+
-
 Staphylococcus aureus dodecanoyl-N-acetyl-cysteamine + NADP+
-
 ?
1.3.1.10 dodecenoyl-[Staphylococcus aureus acyl carrier protein] + NADPH + H+
-
 Staphylococcus aureus dodecanoyl-[Staphylococcus aureus acyl carrier protein] + NADP+
-
 ?
1.3.1.10 additional information no activity with crotonyl-CoA and NADH Staphylococcus aureus ?
-
 ?
1.3.1.10 trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH + H+ prefers acyl carrier protein substrates carrying fatty acids with long acyl chains Staphylococcus aureus acyl-[acyl-carrier protein] + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.10 enoyl reductase
-
 Staphylococcus aureus
1.3.1.10 FabI
-
 Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.10 1.5
-
 Crotonyl-N-acetyl-cysteamine wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 11.6
-
 crotonyl-[Staphylococcus aureus acyl carrier protein] wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 18
-
 dodec-2-enoyl-CoA wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 18.4
-
 dodecenoyl-[Staphylococcus aureus acyl carrier protein] mutant enzyme A95V, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 30.4
-
 dodecenoyl-N-acetyl-cysteamine wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 130.2
-
 NADPH wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 130.2
-
 dodecenoyl-[Staphylococcus aureus acyl carrier protein] wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1.3.1.10 157.3
-
 dodecenoyl-[Staphylococcus aureus acyl carrier protein] mutant enzyme F204S, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.10 NADPH dependent Staphylococcus aureus