EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.113 | expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.113 | O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | Mycobacterium tuberculosis | the specificity of the enzyme for the physiological sulfide equivalent [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH is more than 3 orders of magnitude greater than that for bisulfide | [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.65 | Mycobacterium tuberculosis | - |
isoform CysM | - |
2.5.1.113 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.113 | nickel affinity column chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.65 | additional information | specificity of CysM for its amino acid substrate is more than 500-fold greater for O-phospho-L-serine than for O-acetyl-L-serine. Specificity of CysM for this physiological sulfide equivalent, sulfur carrier protein CysO-COSH, is more than 3 orders of magnitude greater than that for bisulfide. CysM active site with the bound aminoacrylate intermediate is protected from solvent and that binding of CysO-COSH produces a conformational change allowing rapid sulfur transfer | Mycobacterium tuberculosis | ? | - |
? | |
2.5.1.65 | O-acetyl-L-serine + hydrogen sulfide | - |
Mycobacterium tuberculosis | L-cysteine + acetate | - |
? | |
2.5.1.65 | O3-phospho-L-serine + hydrogen sulfide | - |
Mycobacterium tuberculosis | L-cysteine + phosphate | - |
? | |
2.5.1.113 | O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | the specificity of the enzyme for its amino acid substrate is more than 500fold greater for O-phospho-L-serine than for O-acetyl-L-serine | Mycobacterium tuberculosis | ? | - |
? | |
2.5.1.113 | O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | the specificity of the enzyme for the physiological sulfide equivalent [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH is more than 3 orders of magnitude greater than that for bisulfide | Mycobacterium tuberculosis | [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.113 | CysM | - |
Mycobacterium tuberculosis |
2.5.1.113 | Cysteine synthase | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.65 | 0.025 | - |
O3-acetyl-L-serine | formation of the aminoacrylate intermediate | Mycobacterium tuberculosis | |
2.5.1.65 | 17 | - |
O3-phospho-L-serine | formation of the aminoacrylate intermediate | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.113 | pyridoxal 5'-phosphate | - |
Mycobacterium tuberculosis |