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Literature summary extracted from

  • Sucharitakul, J.; Phongsak, T.; Entsch, B.; Svasti, J.; Chaiyen, P.; Ballou, D.P.
    Kinetics of a two-component p-hydroxyphenylacetate hydroxylase explain how reduced flavin is transferred from the reductase to the oxygenase (2007), Biochemistry, 46, 8611-8623.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.9 additional information
-
 additional information steady-steady kinetics, kinetics of FMN reduction, transfer from C1 to C2 component, and FMNH- release, thermodynamic data, including redox potentials, and Kd values, detailed overview Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.9 4-hydroxyphenylacetate + NADH + H+ + O2 Acinetobacter baumannii
-
 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.9 Acinetobacter baumannii
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.14.9 4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O kinetic mechanism of enzyme components C1 and C2 Acinetobacter baumannii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.9 4-hydroxyphenylacetate + NADH + H+ + O2
-
 Acinetobacter baumannii 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + NADH + H+ + O2 HPAH is composed of two proteins: a FMN reductase C1 and an oxygenase C2, C1 catalyzes the reduction of FMN by NADH to generate reduced FMN, FMNH2, for use by C2 in the hydroxylation reaction, C1 is unique among the flavin reductases in that the substrate 4-hydroxyphenylacetate stimulates the rates of both the reduction of FMN and release of FMNH2 from the enzyme, the dissociation of FMNH2 from C1 is rate-limiting in the intermolecular transfer of FMNH2 from C1 to C2, and this process is regulated by the presence of 4-hydroxyphenylacetate , overview Acinetobacter baumannii 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.9 More HPAH is composed of two proteins: a FMN reductase C1 and an oxygenase C2 Acinetobacter baumannii

Synonyms

EC Number Synonyms Comment Organism
1.14.14.9 HPAH
-
 Acinetobacter baumannii
1.14.14.9 p-hydroxyphenylacetate hydroxylase
-
 Acinetobacter baumannii
1.14.14.9 two-component p-hydroxyphenylacetate hydroxylase
-
 Acinetobacter baumannii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.9 25
-
 assay at Acinetobacter baumannii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.9 7
-
 assay at Acinetobacter baumannii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.9 FMN utilized by C1 Acinetobacter baumannii
1.14.14.9 FMNH2 utiluzed by C2 Acinetobacter baumannii
1.14.14.9 NADH
-
 Acinetobacter baumannii