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Literature summary extracted from

  • Wolfe, A.E.; Thymark, M.; Gattis, S.G.; Fagan, R.L.; Hu, Y.C.; Johansson, E.; Arent, S.; Larsen, S.; Palfey, B.A.
    Interaction of benzoate pyrimidine analogues with class 1A dihydroorotate dehydrogenase from Lactococcus lactis (2007), Biochemistry, 46, 5741-5753.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.98.1 expressed in Escherichia coli Lactococcus lactis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.98.1 hanging drop vapour diffusion method, with 30% PEG 6000, 1 mM dithiothreitol, 0.2 M sodium acetate, and 0.1 M Tris-HCl at pH 8.5 Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
1.3.98.1 C130A the mutant forms charge-transfer complexes upon binding 3,4-dihydroxybenzoate, but the maximum of the broad charge-transfer bands is shifted to 590 nm Lactococcus lactis
1.3.98.1 C130S the mutant forms charge-transfer complexes upon binding 3,4-dihydroxybenzoate, but the maximum of the broad charge-transfer bands is shifted to 610 nm Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.98.1 3,4-dihydroxybenzoate
-
 Lactococcus lactis
1.3.98.1 3,5-Dihydroxybenzoate
-
 Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
1.3.98.1 Lactococcus lactis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.98.1
-
 Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.98.1 dihydroorotate + 2,6-dichlorophenolindophenol
-
 Lactococcus lactis orotate + reduced 2,6-dichlorophenolindophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.98.1 class 1A DHOD
-
 Lactococcus lactis
1.3.98.1 class 1A dihydroorotate dehydrogenase
-
 Lactococcus lactis