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Literature summary extracted from

  • Tu, S.L.; Rockwell, N.C.; Lagarias, J.C.; Fisher, A.J.
    Insight into the radical mechanism of phycocyanobilin-ferredoxin oxidoreductase (PcyA) revealed by X-ray crystallography and biochemical measurements (2007), Biochemistry, 46, 1484-1494.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.7.4
-
 Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.7.5 hanging drop vapour diffusion method with 18% PEG-8000, 0.025 M MES-NaOH at pH 6.5, 0.05 M Ca(OAc)2 at pH 6.5, and 5% dioxane (or 5% ethanol) Nostoc sp. PCC 7120 = FACHB-418

Protein Variants

EC Number Protein Variants Comment Organism
1.3.7.5 D102N exhibits 11% of wild type activity Nostoc sp. PCC 7120 = FACHB-418
1.3.7.5 E73Q exhibits 20% of wild type activity Nostoc sp. PCC 7120 = FACHB-418
1.3.7.5 H71Q exhibits 65% of wild type activity Nostoc sp. PCC 7120 = FACHB-418
1.3.7.5 H85Q exhibits 5% of wild type activity Nostoc sp. PCC 7120 = FACHB-418
1.3.7.5 K218E exhibits 20% of wild type activity Nostoc sp. PCC 7120 = FACHB-418

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.4 Arabidopsis thaliana
-
-
-
1.3.7.5 Nostoc sp. PCC 7120 = FACHB-418 Q93TN0 also known as Anabaena sp. strain PCC7120
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.7.4 HPLC Phenomenex Ultracarb reverse phase column chromatography Arabidopsis thaliana
1.3.7.5 Superdex G-200 gel filtration Nostoc sp. PCC 7120 = FACHB-418

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.5 biliverdin IXalpha + reduced ferredoxin
-
 Nostoc sp. PCC 7120 = FACHB-418 phycocyanobilin + oxidized ferredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.7.4 HY2
-
 Arabidopsis thaliana
1.3.7.4 phytochromobilin synthase
-
 Arabidopsis thaliana
1.3.7.4 PphiB synthase
-
 Arabidopsis thaliana
1.3.7.5 PcyA
-
 Nostoc sp. PCC 7120 = FACHB-418
1.3.7.5 phycocyanobilin-ferredoxin oxidoreductase
-
 Nostoc sp. PCC 7120 = FACHB-418