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Literature summary extracted from

  • Hugonnet, J.E.; Blanchard, J.S.
    Irreversible inhibition of the Mycobacterium tuberculosis beta-lactamase by clavulanate (2007), Biochemistry, 46, 11998-12004.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 clavulanate 0.1 mM, irreversible inhibition Mycobacterium tuberculosis
3.5.2.6 Sulbactam 0.1 mM inhibit the enzyme competitively and reversibly with respect to nitrocefin Mycobacterium tuberculosis
3.5.2.6 tazobactam 0.1 mM inhibit the enzyme in a time-dependent manner, but the activity of the enzyme reappears due to the slow hydrolysis of the covalently acylated enzyme Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.0034
-
 meropenem in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.008
-
 ampicillin in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.0094
-
 Imipenem in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.019
-
 penicillin G in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.022
-
 amoxicillin in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.057
-
 nitrocefin in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.059
-
 piperacillin in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.069
-
 Penicillin V in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.114
-
 cephalosporin C in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.127
-
 cefoxitin in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.152
-
 cephalotin in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.184
-
 cefamandole in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.195
-
 CENTA in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.28
-
 ceftazidime in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.52
-
 ceftriaxone in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 5.1
-
 cefuroxime in 100 mM MES (pH 6.4) Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 Ni-NTA agarose column chromatography and Superdex 200 Hi-Load gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 amoxicillin + H2O
-
 Mycobacterium tuberculosis (2R,4S)-2-[(R)-{[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino}(carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Mycobacterium tuberculosis (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefamandole + H2O
-
 Mycobacterium tuberculosis (2R)-2-[(R)-carboxy{[(2R)-2-hydroxy-2-phenylacetyl]amino}methyl]-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Mycobacterium tuberculosis (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Mycobacterium tuberculosis (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 ceftriaxone + H2O
-
 Mycobacterium tuberculosis (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[[(6-hydroxy-2-methyl-5-oxo-2,5-dihydro-1,2,4-triazin-3-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Mycobacterium tuberculosis (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 CENTA + H2O
-
 Mycobacterium tuberculosis (2R)-5-{[(3-carboxy-4-nitrophenyl)sulfanyl]methyl}-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalosporin C + H2O
-
 Mycobacterium tuberculosis N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine
-
 ?
3.5.2.6 cephalotin + H2O
-
 Mycobacterium tuberculosis (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Mycobacterium tuberculosis (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Mycobacterium tuberculosis (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Mycobacterium tuberculosis (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 penicillin G + H2O
-
 Mycobacterium tuberculosis (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 penicillin V + H2O
-
 Mycobacterium tuberculosis (2R,4S)-2-[(R)-carboxy(2-phenoxyacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Mycobacterium tuberculosis (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 Ambler class A beta-lactamase
-
 Mycobacterium tuberculosis
3.5.2.6 BlaC is an an extended spectrum beta-lactamase Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.0013
-
 meropenem in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.033
-
 ceftazidime in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.167
-
 Imipenem in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.8
-
 cefoxitin in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 0.82
-
 ceftriaxone in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 5.67
-
 amoxicillin in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 8.17
-
 cefuroxime in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 8.17
-
 cephalotin in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 9.33
-
 penicillin G in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 11
-
 ampicillin in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 11.5
-
 piperacillin in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 17.83
-
 cephalosporin C in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 29.5
-
 CENTA in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 35
-
 Penicillin V in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 58.3
-
 cefamandole in 100 mM MES (pH 6.4) Mycobacterium tuberculosis
3.5.2.6 111.3
-
 nitrocefin in 100 mM MES (pH 6.4) Mycobacterium tuberculosis