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Literature summary extracted from

  • Sun, Q.A.; Su, D.; Novoselov, S.V.; Carlson, B.A.; Hatfield, D.L.; Gladyshev, V.N.
    Reaction mechanism and regulation of mammalian thioredoxin/glutathione reductase (2005), Biochemistry, 44, 14528-14537.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.B1 recombinant TGR is mostly insoluble when expressed in Escherichi coli, and neither cloning of the enzyme that contain various tags nor coexpression of TGR with chaperones increases its solubility Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.B1 additional information
-
 additional information kinetic properties of the Grx domain of TGR Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.B1 Bos taurus
-
-
-
1.8.1.B1 Danio rerio
-
-
-
1.8.1.B1 Gallus gallus
-
-
-
1.8.1.B1 Homo sapiens
-
-
-
1.8.1.B1 Mus musculus
-
-
-
1.8.1.B1 no activity in Caenorhabditis elegans
-
-
-
1.8.1.B1 no activity in Canis familiaris
-
-
-
1.8.1.B1 no activity in Drosophila melanogaster
-
-
-
1.8.1.B1 no activity in Sus scrofa
-
-
-
1.8.1.B1 Pan troglodytes
-
-
-
1.8.1.B1 Rattus norvegicus
-
-
-
1.8.1.B1 Takifugu rubripes
-
-
-
1.8.1.B1 Tetraodon nigroviridis
-
-
-
1.8.1.B1 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.B1 small amounts of the soluble enzyme are obtained by purifying it from a large quantity of cells, in which protein synthesis is induced slowly for a period of 24 h at a low temperature Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.B1 liver TGR is regulated by both selenium availability and selenocysteine tRNA status. This regulation is more pronounced in liver, whereas in testes, the expression level is only slightly affected Mus musculus
-
1.8.1.B1 testis TGR is regulated by both selenium availability and selenocysteine tRNA status. This regulation is more pronounced in liver, whereas in testes, the expression level is only slightly affected Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.B1 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Mus musculus ?
-
 ?
1.8.1.B1 glutathione disulfide + NADPH + H+
-
 Mus musculus glutathione + NADP+
-
 ?
1.8.1.B1 thioredoxin disulfide + NADPH + H+
-
 Mus musculus thioredoxin + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.B1 TGR
-
 Gallus gallus
1.8.1.B1 TGR
-
 Mus musculus
1.8.1.B1 TGR
-
 Homo sapiens
1.8.1.B1 TGR
-
 Rattus norvegicus
1.8.1.B1 TGR
-
 Bos taurus
1.8.1.B1 TGR
-
 Xenopus laevis
1.8.1.B1 TGR
-
 Danio rerio
1.8.1.B1 TGR
-
 Pan troglodytes
1.8.1.B1 TGR
-
 Takifugu rubripes
1.8.1.B1 TGR
-
 Tetraodon nigroviridis
1.8.1.B1 thioredoxin/glutathione reductase
-
 Gallus gallus
1.8.1.B1 thioredoxin/glutathione reductase
-
 Mus musculus
1.8.1.B1 thioredoxin/glutathione reductase
-
 Homo sapiens
1.8.1.B1 thioredoxin/glutathione reductase
-
 Rattus norvegicus
1.8.1.B1 thioredoxin/glutathione reductase
-
 Bos taurus
1.8.1.B1 thioredoxin/glutathione reductase
-
 Xenopus laevis
1.8.1.B1 thioredoxin/glutathione reductase
-
 Danio rerio
1.8.1.B1 thioredoxin/glutathione reductase
-
 Pan troglodytes
1.8.1.B1 thioredoxin/glutathione reductase
-
 Takifugu rubripes
1.8.1.B1 thioredoxin/glutathione reductase
-
 Tetraodon nigroviridis