EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.B2 | cyanide | suppresses the peculiar endogenous reaction of methanol dehydrogenase, and is also both an activator of substrate-dependent activity and a competitive inhibitor with respect to methanol, the two activities correspond to two distinct binding sites for cyanide, overview | Paracoccus denitrificans | |
1.1.2.B2 | NH4+ | obligatory activator | Paracoccus denitrificans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.B2 | cyanide | suppresses the peculiar endogenous reaction of methanol dehydrogenase, and is also both an activator of substrate-dependent activity and a competitive inhibitor with respect to methanol, the two activities correspond to two distinct binding sites for cyanide, overview | Paracoccus denitrificans | |
1.1.2.B2 | N,N,N',N'-tetramethyl-4-phenylenediamine | i.e. Wurster's blue | Paracoccus denitrificans | |
1.1.2.B2 | phenazine ethosulfate | the articficial cofactor exhibits strong substrate inhibition | Paracoccus denitrificans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.2.B2 | 0.56 | - |
methanol | pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine | Paracoccus denitrificans | |
1.1.2.B2 | 3.22 | - |
N,N,N',N'-tetramethyl-4-phenylenediamine | pH 9.0 30°C | Paracoccus denitrificans | |
1.1.2.B2 | 3.39 | - |
ethanol | pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine | Paracoccus denitrificans | |
1.1.2.B2 | 10.7 | - |
Propanol | pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine | Paracoccus denitrificans | |
1.1.2.B2 | 11.8 | - |
phenazine ethosulfate | pH 9.0, 30°C | Paracoccus denitrificans | |
1.1.2.B2 | 91.4 | - |
butanol | pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine | Paracoccus denitrificans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.B2 | methanol + ferricytochrome c551i | Paracoccus denitrificans | - |
formaldehyde + ferrocytochrome c551i | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.2.B2 | Paracoccus denitrificans | P12293 | P12293 (large subunit, alpha) and P12293 (small subunit, beta) | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.2.B2 | 7.6 | - |
- |
Paracoccus denitrificans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.B2 | butanol + N,N,N',N'-tetramethyl-4-phenylenediamine | - |
Paracoccus denitrificans | butyraldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine | - |
? | |
1.1.2.B2 | ethanol + N,N,N',N'-tetramethyl-4-phenylenediamine | - |
Paracoccus denitrificans | acetaldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine | - |
? | |
1.1.2.B2 | methanol + 2,6-dichlorophenolindophenol | - |
Paracoccus denitrificans | formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.2.B2 | methanol + ferricytochrome c551i | - |
Paracoccus denitrificans | formaldehyde + ferrocytochrome c551i | - |
? | |
1.1.2.B2 | methanol + N,N,N',N'-tetramethyl-4-phenylenediamine | - |
Paracoccus denitrificans | formaldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine | - |
? | |
1.1.2.B2 | methanol + phenazine ethosulfate | phenazine ethosulfate is a two-electron acceptor | Paracoccus denitrificans | formaldehyde + reduced phenazine ethosulfate | - |
? | |
1.1.2.B2 | propanol + N,N,N',N'-tetramethyl-4-phenylenediamine | - |
Paracoccus denitrificans | propionaldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.2.B2 | quinoprotein methanol dehydrogenase | - |
Paracoccus denitrificans |
1.1.5.5 | quinoprotein methanol dehydrogenase | - |
- |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.2.B2 | 30 | - |
assay at | Paracoccus denitrificans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.2.B2 | 9 | - |
assay at | Paracoccus denitrificans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.B2 | pyrroloquinoline quinone | - |
Paracoccus denitrificans |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.2.B2 | 0.59 | - |
phenazine ethosulfate | pH 9.0, 30°C | Paracoccus denitrificans | |
1.1.2.B2 | 9.37 | - |
N,N,N',N'-tetramethyl-4-phenylenediamine | pH 9.0 30°C | Paracoccus denitrificans |