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Literature summary extracted from

  • Harris, T.K.; Davidson, V.L.
    A new kinetic model for the steady-state reactions of the quinoprotein methanol dehydrogenase from Paracoccus denitrificans (1993), Biochemistry, 32, 4362-4368.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.2.B2 cyanide suppresses the peculiar endogenous reaction of methanol dehydrogenase, and is also both an activator of substrate-dependent activity and a competitive inhibitor with respect to methanol, the two activities correspond to two distinct binding sites for cyanide, overview Paracoccus denitrificans
1.1.2.B2 NH4+ obligatory activator Paracoccus denitrificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.2.B2 cyanide suppresses the peculiar endogenous reaction of methanol dehydrogenase, and is also both an activator of substrate-dependent activity and a competitive inhibitor with respect to methanol, the two activities correspond to two distinct binding sites for cyanide, overview Paracoccus denitrificans
1.1.2.B2 N,N,N',N'-tetramethyl-4-phenylenediamine i.e. Wurster's blue Paracoccus denitrificans
1.1.2.B2 phenazine ethosulfate the articficial cofactor exhibits strong substrate inhibition Paracoccus denitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.B2 0.56
-
methanol pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine Paracoccus denitrificans
1.1.2.B2 3.22
-
N,N,N',N'-tetramethyl-4-phenylenediamine pH 9.0 30°C Paracoccus denitrificans
1.1.2.B2 3.39
-
ethanol pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine Paracoccus denitrificans
1.1.2.B2 10.7
-
Propanol pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine Paracoccus denitrificans
1.1.2.B2 11.8
-
phenazine ethosulfate pH 9.0, 30°C Paracoccus denitrificans
1.1.2.B2 91.4
-
butanol pH 9.0, 30°C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.B2 methanol + ferricytochrome c551i Paracoccus denitrificans
-
formaldehyde + ferrocytochrome c551i
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.B2 Paracoccus denitrificans P12293 P12293 (large subunit, alpha) and P12293 (small subunit, beta)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.B2 7.6
-
-
Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.B2 butanol + N,N,N',N'-tetramethyl-4-phenylenediamine
-
Paracoccus denitrificans butyraldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine
-
?
1.1.2.B2 ethanol + N,N,N',N'-tetramethyl-4-phenylenediamine
-
Paracoccus denitrificans acetaldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine
-
?
1.1.2.B2 methanol + 2,6-dichlorophenolindophenol
-
Paracoccus denitrificans formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
1.1.2.B2 methanol + ferricytochrome c551i
-
Paracoccus denitrificans formaldehyde + ferrocytochrome c551i
-
?
1.1.2.B2 methanol + N,N,N',N'-tetramethyl-4-phenylenediamine
-
Paracoccus denitrificans formaldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine
-
?
1.1.2.B2 methanol + phenazine ethosulfate phenazine ethosulfate is a two-electron acceptor Paracoccus denitrificans formaldehyde + reduced phenazine ethosulfate
-
?
1.1.2.B2 propanol + N,N,N',N'-tetramethyl-4-phenylenediamine
-
Paracoccus denitrificans propionaldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.B2 quinoprotein methanol dehydrogenase
-
Paracoccus denitrificans
1.1.5.5 quinoprotein methanol dehydrogenase
-
-

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.B2 30
-
assay at Paracoccus denitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.B2 9
-
assay at Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.B2 pyrroloquinoline quinone
-
Paracoccus denitrificans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.2.B2 0.59
-
phenazine ethosulfate pH 9.0, 30°C Paracoccus denitrificans
1.1.2.B2 9.37
-
N,N,N',N'-tetramethyl-4-phenylenediamine pH 9.0 30°C Paracoccus denitrificans