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Literature summary extracted from

  • Munoz-Munoz, J.L.; Garcia-Molina, F.; Garcia-Ruiz, P.A.; Molina-Alarcon, M.; Tudela, J.; Garcia-Canovas, F.; Rodriguez-Lopez, J.N.
    Phenolic substrates and suicide inactivation of tyrosinase: kinetics and mechanism (2008), Biochem. J., 416, 431-440.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.18.1 pharmacology study of suicide inactivation and irreversible inhibition is important in the functional design of synthetic inactivators for therapeutic applications Agaricus bisporus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 additional information suicide inactivation of tyrosinase acting on o-diphenols. A kinetic study of the suicide inactivation of tyrosinase during its action on a variety of substrates is described, and a mechanism is proposed to explain the experimental kinetic results and to throw light on the suicide inactivation step of mushroom tyrosinase Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.07
-
 protocatechuic acid
-
 Agaricus bisporus
1.14.18.1 0.1
-
 4-methylcatechol
-
 Agaricus bisporus
1.14.18.1 0.1
-
 4-nitrocatechol
-
 Agaricus bisporus
1.14.18.1 0.12
-
 methyl gallate
-
 Agaricus bisporus
1.14.18.1 0.15
-
 Protocatechuic aldehyde
-
 Agaricus bisporus
1.14.18.1 0.16
-
 catechol
-
 Agaricus bisporus
1.14.18.1 0.17
-
 4-Ethylcatechol
-
 Agaricus bisporus
1.14.18.1 0.18
-
 gallic acid
-
 Agaricus bisporus
1.14.18.1 0.6
-
 4-Chlorocatechol
-
 Agaricus bisporus
1.14.18.1 0.7
-
 3,4-Dihydroxyphenylpropionic acid
-
 Agaricus bisporus
1.14.18.1 1.3
-
 3,4-dihydroxyphenylacetic acid
-
 Agaricus bisporus
1.14.18.1 1.45
-
 4-tert-butylcatechol
-
 Agaricus bisporus
1.14.18.1 1.96
-
 pyrogallol
-
 Agaricus bisporus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 copper
-
 Agaricus bisporus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Agaricus bisporus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1
-
 Agaricus bisporus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 commercial preparation
-
 Agaricus bisporus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 8300
-
 mushroom tyrosinase 8300 U/mg Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 3,4-dihydroxyphenylacetic acid + 1/2 O2 DHPAA Agaricus bisporus (3,4-dioxocyclohexa-1,5-dien-1-yl)acetic acid + H2O
-
 ?
1.14.18.1 3,4-dihydroxyphenylpropionic acid + 1/2 O2 DHPPA Agaricus bisporus 3-(3,4-dioxocyclohexa-1,5-dien-1-yl)propanoic acid + H2O
-
 ?
1.14.18.1 4-chlorocatechol + 1/2 O2
-
 Agaricus bisporus 4-chlorocyclohexa-3,5-diene-1,2-dione + H2O
-
 ?
1.14.18.1 4-ethylcatechol + 1/2 O2
-
 Agaricus bisporus 4-ethylcyclohexa-3,5-diene-1,2-dione + H2O
-
 ?
1.14.18.1 4-methylcatechol + 1/2 O2
-
 Agaricus bisporus 4-methyl-1,2-benzoquinone + H2O
-
 ?
1.14.18.1 4-nitrocatechol + 1/2 O2
-
 Agaricus bisporus 4-nitrocyclohexa-3,5-diene-1,2-dione + H2O
-
 ?
1.14.18.1 4-tert-butylcatechol + 1/2 O2
-
 Agaricus bisporus 4-tert-butylcyclohexa-3,5-diene-1,2-dione + H2O
-
 ?
1.14.18.1 catechol + 1/2 O2 pyrogallol and catechol are best substrates for catalysis and inactivation Agaricus bisporus 1,2-benzoquinone + H2O
-
 ?
1.14.18.1 gallic acid + 1/2 O2
-
 Agaricus bisporus 5-hydroxy-3,4-dioxocyclohexa-1,5-diene-1-carboxylic acid + H2O
-
 ?
1.14.18.1 methyl gallate + O2
-
 Agaricus bisporus methyl 5-hydroxy-3,4-dioxocyclohexa-1,5-diene-1-carboxylate + H2O
-
 ?
1.14.18.1 protocatechuic acid + 1/2 O2
-
 Agaricus bisporus 3,4-dioxocyclohexa-1,5-diene-1-carboxylic acid + H2O
-
 ?
1.14.18.1 protocatechuic aldehyde + 1/2 O2
-
 Agaricus bisporus 3,4-dioxocyclohexa-1,5-diene-1-carbaldehyde + H2O
-
 ?
1.14.18.1 pyrogallol + 1/2 O2 pyrogallol and catechol are best substrates for catalysis and inactivation Agaricus bisporus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 o-diphenol: oxidoreductase
-
 Agaricus bisporus
1.14.18.1 polyphenol oxidase
-
 Agaricus bisporus
1.14.18.1 tyrosinase
-
 Agaricus bisporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 25
-
 assay at Agaricus bisporus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.18.1 0.9
-
 4-nitrocatechol
-
 Agaricus bisporus
1.14.18.1 1.5
-
 Protocatechuic aldehyde
-
 Agaricus bisporus
1.14.18.1 8.1
-
 protocatechuic acid
-
 Agaricus bisporus
1.14.18.1 28.2
-
 gallic acid
-
 Agaricus bisporus
1.14.18.1 80.2
-
 methyl gallate
-
 Agaricus bisporus
1.14.18.1 433.1
-
 3,4-dihydroxyphenylacetic acid
-
 Agaricus bisporus
1.14.18.1 607.2
-
 3,4-Dihydroxyphenylpropionic acid
-
 Agaricus bisporus
1.14.18.1 640.1
-
 4-tert-butylcatechol
-
 Agaricus bisporus
1.14.18.1 802.3
-
 4-Ethylcatechol
-
 Agaricus bisporus
1.14.18.1 842.1
-
 4-methylcatechol
-
 Agaricus bisporus
1.14.18.1 859.2
-
 4-Chlorocatechol
-
 Agaricus bisporus
1.14.18.1 874.1
-
 catechol
-
 Agaricus bisporus
1.14.18.1 1280
-
 pyrogallol
-
 Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 7
-
 assay at Agaricus bisporus