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Literature summary extracted from

  • Sawicki, A.; Willows, R.D.
    S-adenosyl-L-methionine:magnesium-protoporphyrin IX O-methyltransferase from Rhodobacter capsulatus: mechanistic insights and stimulation with phospholipids (2007), Biochem. J., 406, 469-478.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.11 Phospholipids increase enzymatic activity 3-4fold Rhodobacter capsulatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.11 derived from plasmid pRPS404, consisting of a 46 kb pair section of the photosynthetic gene cluster, overexpression of His6-tagged protein in Escherichia coli, strain BL21DE3, expression plasmid pHisBchM Rhodobacter capsulatus

General Stability

EC Number General Stability Organism
2.1.1.11 phosphatidylglycerol stabilizes enzymatic activity causing disaggregation to lower molecular mass forms Rhodobacter capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.11 cobalt II-protoporphyrin non-substrate metalloporphyrin, competitive, assay described Rhodobacter capsulatus
2.1.1.11 magnesium protoporphyrin monomethyl ester non-competitive product inhibition Rhodobacter capsulatus
2.1.1.11 manganese III-protoporphyrin non-substrate metalloporphyrin, non-competitive or un-competitive, assay described Rhodobacter capsulatus
2.1.1.11 additional information higher magnesium ion concentrations above 3.8 mM inhibit enzyme activity in presence or absence of magnesium-chelatase subunits or BSA Rhodobacter capsulatus
2.1.1.11 S-adenosyl-L-homocysteine non-competitive product inhibition Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.11 0.0006
-
 magnesium deuteroporphyrin determined using 250 microM S-adenosyl-L-methionine Rhodobacter capsulatus
2.1.1.11 0.0008
-
 zinc protoporphyrin determined using 250 microM S-adenosyl-L-methionine Rhodobacter capsulatus
2.1.1.11 0.001
-
 magnesium mesoporphyrin determined using 250 microM S-adenosyl-L-methionine Rhodobacter capsulatus
2.1.1.11 0.045
-
 S-adenosyl-L-methionine previously determined constant Rhodobacter capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.11 29000
-
 His6-tagged protein, gel filtration, SDS-PAGE Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.11 S-adenosyl-L-methionine + magnesium mesoporphyrin Rhodobacter capsulatus
-
 S-adenosyl-L-homocysteine + magnesium mesoporphyrin monomethyl ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.11 Rhodobacter capsulatus P26236
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.11 from inclusion bodies, gel filtration and SDS-PAGE Rhodobacter capsulatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.11 cell culture
-
 Rhodobacter capsulatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.11 additional information
-
 effect of detergents and alcohols on activity analyzed, no stimulatory effect by magnesium-chelatase subunits, heat-stable stimulatory component present in S-adenosyl-L-methionine synthetase found to be a phospholipid, kinetic analysis shows a random sequential reaction mechanism Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.11 S-adenosyl-L-methionine + magnesium deuteroporphyrin in situ generated S-adenosyl-L-methionine substrate reveals higher enzymatic activity in assay, substrate specificity limited to 5-co-ordinate squarepyramidal metalloporphyrins Rhodobacter capsulatus S-adenosyl-L-homocysteine + magnesium deuteroporphyrin monomethyl ester
-
 ?
2.1.1.11 S-adenosyl-L-methionine + magnesium mesoporphyrin
-
 Rhodobacter capsulatus S-adenosyl-L-homocysteine + magnesium mesoporphyrin monomethyl ester
-
 ?
2.1.1.11 S-adenosyl-L-methionine + magnesium mesoporphyrin in situ generated S-adenosyl-L-methionine substrate reveals higher enzymatic activity in assay Rhodobacter capsulatus S-adenosyl-L-homocysteine + magnesium mesoporphyrin monomethyl ester
-
 ?
2.1.1.11 S-adenosyl-L-methionine + magnesium protoporphyrin in situ generated S-adenosyl-L-methionine substrate reveals higher enzymatic activity in assay Rhodobacter capsulatus S-adenosyl-L-homocysteine + magnesium protoporphyrin monomethyl ester
-
 ?
2.1.1.11 S-adenosyl-L-methionine + zinc protoporphyrin in situ generated S-adenosyl-L-methionine substrate reveals higher enzymatic activity in assay, substrate specificity limited to 5-co-ordinate squarepyramidal metalloporphyrins Rhodobacter capsulatus S-adenosyl-L-homocysteine + zinc protoporphyrin monomethyl ester
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.11 More disaggregation to lower molecular mass forms, monomeric to multimeric species, gel filtration Rhodobacter capsulatus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.11 BchM
-
 Rhodobacter capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.11 30
-
 assay at Rhodobacter capsulatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.11 0.003
-
 magnesium deuteroporphyrin determined using 250 microM S-adenosyl-L-methionine Rhodobacter capsulatus
2.1.1.11 0.018
-
 zinc protoporphyrin determined using 250 microM S-adenosyl-L-methionine Rhodobacter capsulatus
2.1.1.11 0.04
-
 magnesium protoporphyrin determined using 250 microM S-adenosyl-L-methionine Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.11 8
-
 slight preference for Tris/HCl buffer over Tricine/NaOH Rhodobacter capsulatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.11 7.5 8.5 within activity range Rhodobacter capsulatus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.11 0.00045
-
 magnesium protoporphyrin monomethyl ester non-competitive product inhibition, magnesium deuteroporphyrin as substrate Rhodobacter capsulatus
2.1.1.11 0.00061
-
 magnesium protoporphyrin monomethyl ester non-competitive product inhibition, S-adenosyl-L-methionine as substrate Rhodobacter capsulatus
2.1.1.11 0.121
-
 S-adenosyl-L-homocysteine non-competitive, magnesium protoporphyrin as substrate Rhodobacter capsulatus
2.1.1.11 0.141
-
 S-adenosyl-L-homocysteine non-competitive, S-adenosyl-L-methionine as substrate Rhodobacter capsulatus