BRENDA - Enzyme Database show

Purification and characterization of a Baeyer-Villiger mono-oxygenase from Rhodococcus erythropolis DCL14 involved in three different monocyclic monoterpene degradation pathways

van der Werf, M.J.; Biochem. J. 347 Pt 3, 693-701 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.105
ATP
-
Rhodococcus erythropolis
1.14.13.105
HgCl2
1 mM, 85% inhibition
Rhodococcus erythropolis
1.14.13.105
additional information
no inhibition with 1 mM iodoacetate, iodoacetamide, EDTA, 2,2'-dipyridyl and DTT
Rhodococcus erythropolis
1.14.13.105
p-chloromercuriobenzoate
0.1 mM, 14% inhibition
Rhodococcus erythropolis
1.14.13.105
phenylhydrazine
1 mM, 10% inhibition
Rhodococcus erythropolis
1.14.13.105
SDS
1 mM, 97% inhibition
Rhodococcus erythropolis
1.14.13.105
ZnCl2
1 mM 33% inhibition
Rhodococcus erythropolis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.105
0.038
-
NADPH
-
Rhodococcus erythropolis
1.14.13.105
0.12
-
(1R,4S)-menthone
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
1.14.13.105
0.13
-
(1S,4R)-1-hydroxy-2-oxolimonene
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
1.14.13.105
0.13
-
(4R)-dihydrocarvone
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.13.105
57000
-
gel filtration
Rhodococcus erythropolis
1.14.13.105
60000
-
1 * 60000, SDS-PAGE
Rhodococcus erythropolis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.105
additional information
Rhodococcus erythropolis
MMKMO is involved in the conversion of the monocyclic monoterpene ketone intermediates formed in the degradation pathways of all stereoisomers of three different monocyclic monoterpenes, i.e. limonene, (dihydro)carveol and menthol. MMKMO converts all enantiomers of the natural substrates with almost equal efficiency
?
-
-
-
1.14.13.105
additional information
Rhodococcus erythropolis DCL14
MMKMO is involved in the conversion of the monocyclic monoterpene ketone intermediates formed in the degradation pathways of all stereoisomers of three different monocyclic monoterpenes, i.e. limonene, (dihydro)carveol and menthol. MMKMO converts all enantiomers of the natural substrates with almost equal efficiency
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.105
Rhodococcus erythropolis
-
-
-
1.14.13.105
Rhodococcus erythropolis DCL14
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.13.105
-
Rhodococcus erythropolis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.105
(1R)-pulegone + NADPH + H+ + O2
31% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
(1R)-pulegone + NADPH + H+ + O2
31% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis DCL14
?
-
-
-
?
1.14.13.105
(1R,4R)-dihydrocarvone + NADPH + H+ + O2
-
684953
Rhodococcus erythropolis
(4R,7R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + NADP+ + H2O
-
-
-
?
1.14.13.105
(1R,4R)-dihydrocarvone + NADPH + H+ + O2
-
684953
Rhodococcus erythropolis DCL14
(4R,7R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + NADP+ + H2O
-
-
-
?
1.14.13.105
(1R,4S)-1-hydroxy-2-oxolimonene + NADPH + H+ + O2
3-isopropenyl-6-oxoheptanoate is the spontaneous rearrangement product of the lactone formed by MMKMO. 133% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
3-isopropenyl-6-oxoheptanoate + NADP+ + H2O
-
-
-
?
1.14.13.105
(1R,4S)-1-hydroxy-2-oxolimonene + NADPH + H+ + O2
3-isopropenyl-6-oxoheptanoate is the spontaneous rearrangement product of the lactone formed by MMKMO. 133% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis DCL14
3-isopropenyl-6-oxoheptanoate + NADP+ + H2O
-
-
-
?
1.14.13.105
(1R,4S)-menthone + NADPH + H+ + O2
76% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
(4R,7S)-7-isopropyl-4-methyl-2-oxo-oxepanone + NADP+ + H2O
-
-
-
?
1.14.13.105
(1S)-pulegone + NADPH + H+ + O2
51% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
(1S,4R)-1-hydroxy-2-oxolimonene + NADPH + H+ + O2
3-isopropenyl-6-oxoheptanoate is the spontaneous rearrangement product of the lactone formed by MMKMO. 88% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
3-isopropenyl-6-oxoheptanoate + NADP+ + H2O
-
-
-
?
1.14.13.105
(1S,4R)-dihydrocarvone + NADPH + H+ + O2
-
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
(1S,4R)-menthone + NADPH + H+ + O2
82% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
(4S,7R)-7-isopropyl-4-methyl-2-oxo-oxepanone + NADP+ + H2O
-
-
-
?
1.14.13.105
(1S,4R)iso-dihydrocarvone + NADPH + H+ + O2
-
684953
Rhodococcus erythropolis
(3S,4R)-6-isopropenyl-3-methyl-2-oxo-oxepanone + NADP+ + H2O
-
-
-
?
1.14.13.105
(4S)-carvone + NADPH + H+ + O2
14% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
1,2-cyclohexanedione + NADPH + H+ + O2
95% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
1,4-cyclohexanedione + NADPH + H+ + O2
92% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2,2-dimethylcyclohexanone + NADPH + H+ + O2
161% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2,6-dimethylcyclohexanone + NADPH + H+ + O2
108% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2-allylcyclohexanone + NADPH + H+ + O2
145% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2-chlorocyclohexanone + NADPH + H+ + O2
112% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2-methoxycyclohexanone + NADPH + H+ + O2
168% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2-methylcyclohexanone + NADPH + H+ + O2
155% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2-phenylcyclohexanone + NADPH + O2
148% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
3-methylcyclohexanone + NADPH + H+ + O2
151% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
4-methylcyclohexanone + NADPH + H+ + O2
40% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
8-mercaptomenthone + NADPH + H+ + O2
14% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
cycloheptanone + NADPH + H+ + O2
10% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
cyclohexanone + NADPH + H+ + O2
151% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
cyclopentanone + NADPH + H+ + O2
12% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
additional information
MMKMO is involved in the conversion of the monocyclic monoterpene ketone intermediates formed in the degradation pathways of all stereoisomers of three different monocyclic monoterpenes, i.e. limonene, (dihydro)carveol and menthol. MMKMO converts all enantiomers of the natural substrates with almost equal efficiency
684953
Rhodococcus erythropolis
?
-
-
-
-
1.14.13.105
additional information
MMKMO converts all enantiomers of the natural substrates with almost equal efficiency. No activity with NADH
684953
Rhodococcus erythropolis
?
-
-
-
-
1.14.13.105
additional information
MMKMO is involved in the conversion of the monocyclic monoterpene ketone intermediates formed in the degradation pathways of all stereoisomers of three different monocyclic monoterpenes, i.e. limonene, (dihydro)carveol and menthol. MMKMO converts all enantiomers of the natural substrates with almost equal efficiency
684953
Rhodococcus erythropolis DCL14
?
-
-
-
-
1.14.13.105
additional information
MMKMO converts all enantiomers of the natural substrates with almost equal efficiency. No activity with NADH
684953
Rhodococcus erythropolis DCL14
?
-
-
-
-
1.14.13.105
norcamphor + NADPH + H+ + O2
145% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.13.105
monomer
1 * 60000, SDS-PAGE
Rhodococcus erythropolis
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.14.13.105
36
-
-
Rhodococcus erythropolis
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.14.13.105
40
-
inactivation above
Rhodococcus erythropolis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.13.105
3.6
-
(1R,4S)-menthone
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
1.14.13.105
3.9
-
(1S,4R)-1-hydroxy-2-oxolimonene
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
1.14.13.105
3.9
-
(4R)-dihydrocarvone
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
1.14.13.105
4.9
-
(1R,4S)-1-hydroxy-2-oxolimonene
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
1.14.13.105
6
-
(1S,4R)-menthone
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.105
8.3
-
pH-optimum in Tris/HCl buffer
Rhodococcus erythropolis
1.14.13.105
9.5
-
pH-optimum in glycine/NaOH buffer
Rhodococcus erythropolis
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.14.13.105
8.5
-
4 min, inactivation of MMKMO in Tris/HCl buffer is observed above pH 8.5
Rhodococcus erythropolis
1.14.13.105
10
-
4 min, inactivation of MMKMO in glycine/NaOH buffer is observed above pH 8.5
Rhodococcus erythropolis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.105
FAD
contains 1 mol FAD per monomer as prosthetic group
Rhodococcus erythropolis
1.14.13.105
NADPH
no activity with NADH
Rhodococcus erythropolis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.13.105
9.5
-
ATP
-
Rhodococcus erythropolis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.105
FAD
contains 1 mol FAD per monomer as prosthetic group
Rhodococcus erythropolis
1.14.13.105
NADPH
no activity with NADH
Rhodococcus erythropolis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.105
ATP
-
Rhodococcus erythropolis
1.14.13.105
HgCl2
1 mM, 85% inhibition
Rhodococcus erythropolis
1.14.13.105
additional information
no inhibition with 1 mM iodoacetate, iodoacetamide, EDTA, 2,2'-dipyridyl and DTT
Rhodococcus erythropolis
1.14.13.105
p-chloromercuriobenzoate
0.1 mM, 14% inhibition
Rhodococcus erythropolis
1.14.13.105
phenylhydrazine
1 mM, 10% inhibition
Rhodococcus erythropolis
1.14.13.105
SDS
1 mM, 97% inhibition
Rhodococcus erythropolis
1.14.13.105
ZnCl2
1 mM 33% inhibition
Rhodococcus erythropolis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.13.105
9.5
-
ATP
-
Rhodococcus erythropolis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.105
0.038
-
NADPH
-
Rhodococcus erythropolis
1.14.13.105
0.12
-
(1R,4S)-menthone
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
1.14.13.105
0.13
-
(1S,4R)-1-hydroxy-2-oxolimonene
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
1.14.13.105
0.13
-
(4R)-dihydrocarvone
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.13.105
57000
-
gel filtration
Rhodococcus erythropolis
1.14.13.105
60000
-
1 * 60000, SDS-PAGE
Rhodococcus erythropolis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.105
additional information
Rhodococcus erythropolis
MMKMO is involved in the conversion of the monocyclic monoterpene ketone intermediates formed in the degradation pathways of all stereoisomers of three different monocyclic monoterpenes, i.e. limonene, (dihydro)carveol and menthol. MMKMO converts all enantiomers of the natural substrates with almost equal efficiency
?
-
-
-
1.14.13.105
additional information
Rhodococcus erythropolis DCL14
MMKMO is involved in the conversion of the monocyclic monoterpene ketone intermediates formed in the degradation pathways of all stereoisomers of three different monocyclic monoterpenes, i.e. limonene, (dihydro)carveol and menthol. MMKMO converts all enantiomers of the natural substrates with almost equal efficiency
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.105
-
Rhodococcus erythropolis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.105
(1R)-pulegone + NADPH + H+ + O2
31% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
(1R)-pulegone + NADPH + H+ + O2
31% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis DCL14
?
-
-
-
?
1.14.13.105
(1R,4R)-dihydrocarvone + NADPH + H+ + O2
-
684953
Rhodococcus erythropolis
(4R,7R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + NADP+ + H2O
-
-
-
?
1.14.13.105
(1R,4R)-dihydrocarvone + NADPH + H+ + O2
-
684953
Rhodococcus erythropolis DCL14
(4R,7R)-4-isopropenyl-7-methyl-2-oxo-oxepanone + NADP+ + H2O
-
-
-
?
1.14.13.105
(1R,4S)-1-hydroxy-2-oxolimonene + NADPH + H+ + O2
3-isopropenyl-6-oxoheptanoate is the spontaneous rearrangement product of the lactone formed by MMKMO. 133% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
3-isopropenyl-6-oxoheptanoate + NADP+ + H2O
-
-
-
?
1.14.13.105
(1R,4S)-1-hydroxy-2-oxolimonene + NADPH + H+ + O2
3-isopropenyl-6-oxoheptanoate is the spontaneous rearrangement product of the lactone formed by MMKMO. 133% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis DCL14
3-isopropenyl-6-oxoheptanoate + NADP+ + H2O
-
-
-
?
1.14.13.105
(1R,4S)-menthone + NADPH + H+ + O2
76% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
(4R,7S)-7-isopropyl-4-methyl-2-oxo-oxepanone + NADP+ + H2O
-
-
-
?
1.14.13.105
(1S)-pulegone + NADPH + H+ + O2
51% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
(1S,4R)-1-hydroxy-2-oxolimonene + NADPH + H+ + O2
3-isopropenyl-6-oxoheptanoate is the spontaneous rearrangement product of the lactone formed by MMKMO. 88% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
3-isopropenyl-6-oxoheptanoate + NADP+ + H2O
-
-
-
?
1.14.13.105
(1S,4R)-dihydrocarvone + NADPH + H+ + O2
-
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
(1S,4R)-menthone + NADPH + H+ + O2
82% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
(4S,7R)-7-isopropyl-4-methyl-2-oxo-oxepanone + NADP+ + H2O
-
-
-
?
1.14.13.105
(1S,4R)iso-dihydrocarvone + NADPH + H+ + O2
-
684953
Rhodococcus erythropolis
(3S,4R)-6-isopropenyl-3-methyl-2-oxo-oxepanone + NADP+ + H2O
-
-
-
?
1.14.13.105
(4S)-carvone + NADPH + H+ + O2
14% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
1,2-cyclohexanedione + NADPH + H+ + O2
95% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
1,4-cyclohexanedione + NADPH + H+ + O2
92% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2,2-dimethylcyclohexanone + NADPH + H+ + O2
161% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2,6-dimethylcyclohexanone + NADPH + H+ + O2
108% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2-allylcyclohexanone + NADPH + H+ + O2
145% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2-chlorocyclohexanone + NADPH + H+ + O2
112% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2-methoxycyclohexanone + NADPH + H+ + O2
168% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2-methylcyclohexanone + NADPH + H+ + O2
155% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
2-phenylcyclohexanone + NADPH + O2
148% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
3-methylcyclohexanone + NADPH + H+ + O2
151% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
4-methylcyclohexanone + NADPH + H+ + O2
40% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
8-mercaptomenthone + NADPH + H+ + O2
14% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
cycloheptanone + NADPH + H+ + O2
10% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
cyclohexanone + NADPH + H+ + O2
151% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
cyclopentanone + NADPH + H+ + O2
12% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
1.14.13.105
additional information
MMKMO is involved in the conversion of the monocyclic monoterpene ketone intermediates formed in the degradation pathways of all stereoisomers of three different monocyclic monoterpenes, i.e. limonene, (dihydro)carveol and menthol. MMKMO converts all enantiomers of the natural substrates with almost equal efficiency
684953
Rhodococcus erythropolis
?
-
-
-
-
1.14.13.105
additional information
MMKMO converts all enantiomers of the natural substrates with almost equal efficiency. No activity with NADH
684953
Rhodococcus erythropolis
?
-
-
-
-
1.14.13.105
additional information
MMKMO is involved in the conversion of the monocyclic monoterpene ketone intermediates formed in the degradation pathways of all stereoisomers of three different monocyclic monoterpenes, i.e. limonene, (dihydro)carveol and menthol. MMKMO converts all enantiomers of the natural substrates with almost equal efficiency
684953
Rhodococcus erythropolis DCL14
?
-
-
-
-
1.14.13.105
additional information
MMKMO converts all enantiomers of the natural substrates with almost equal efficiency. No activity with NADH
684953
Rhodococcus erythropolis DCL14
?
-
-
-
-
1.14.13.105
norcamphor + NADPH + H+ + O2
145% of the activity with (1R,4R)-dihydrocarvone
684953
Rhodococcus erythropolis
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.13.105
monomer
1 * 60000, SDS-PAGE
Rhodococcus erythropolis
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.14.13.105
36
-
-
Rhodococcus erythropolis
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.14.13.105
40
-
inactivation above
Rhodococcus erythropolis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.13.105
3.6
-
(1R,4S)-menthone
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
1.14.13.105
3.9
-
(1S,4R)-1-hydroxy-2-oxolimonene
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
1.14.13.105
3.9
-
(4R)-dihydrocarvone
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
1.14.13.105
4.9
-
(1R,4S)-1-hydroxy-2-oxolimonene
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
1.14.13.105
6
-
(1S,4R)-menthone
30C and glyine/NaOH buffer, pH 9.5
Rhodococcus erythropolis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.105
8.3
-
pH-optimum in Tris/HCl buffer
Rhodococcus erythropolis
1.14.13.105
9.5
-
pH-optimum in glycine/NaOH buffer
Rhodococcus erythropolis
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.14.13.105
8.5
-
4 min, inactivation of MMKMO in Tris/HCl buffer is observed above pH 8.5
Rhodococcus erythropolis
1.14.13.105
10
-
4 min, inactivation of MMKMO in glycine/NaOH buffer is observed above pH 8.5
Rhodococcus erythropolis