EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.2.7 | additional information | construction of Ca2+-lacking, catalytically inactive mutant apoenzymes, reconstitution of the active holoenzyme by incorporation of two exogenous Ca2+ into the active sites of the alpha-subunits of the alpha2beta2 tetramer | Methylorubrum extorquens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.2.7 | periplasm | - |
Methylorubrum extorquens | - |
- |
1.1.2.7 | soluble | - |
Methylorubrum extorquens | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.7 | Ba2+ | Ca2+ can be replaced in the incorporation process by strontium or barium, the affinities for these ions being similar to that for Ca2+, Ba2+ shows 102% of the activity with Ca2+ | Methylorubrum extorquens | |
1.1.2.7 | Ca2+ | 2 Ca2+ ions are irreversibly incorporated per alpha2beta2 tetramer. Calcium can be replaced in the incorporation process by strontium or barium, the affinities for these ions being similar to that for Ca2+ | Methylorubrum extorquens | |
1.1.2.7 | Sr2+ | Ca2+ can be replaced in the incorporation process by strontium or barium, the affinities for these ions being similar to that for Ca2+, Sr2+ shows 94% of the activity with Ca2+ | Methylorubrum extorquens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.7 | methanol + ferricytochrome cL | Methylorubrum extorquens | - |
formaldehyde + ferrocytochrome cL | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.2.7 | Methylorubrum extorquens | P16027 and P14775 | P16027 (large subunit, alpha) and P14775 (small subunit, beta); gene mxaA | - |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.1.2.7 | reconstitution of the active holoenzyme by incorporation of two exogenous Ca2+ into the active sites of the alpha-subunits of the alpha2beta2 tetramer, time course of Ca2+ incorporation, overview | Methylorubrum extorquens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.7 | methanol + ferricytochrome cL | - |
Methylorubrum extorquens | formaldehyde + ferrocytochrome cL | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.2.7 | quinoprotein methanol dehydrogenase | - |
Methylorubrum extorquens |
1.1.5.5 | quinoprotein methanol dehydrogenase | - |
- |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.2.7 | 25 | - |
assay at | Methylorubrum extorquens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.2.7 | 10 | - |
about | Methylorubrum extorquens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.2.7 | 7 | 10.5 | pH profile, overview | Methylorubrum extorquens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.7 | cytochrome cL | electron acceptor | Methylorubrum extorquens | |
1.1.2.7 | pyrroloquinoline quinone | PQQ, prosthetic group | Methylorubrum extorquens |