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Literature summary extracted from

  • Gu, Y.; Yu, R.K.
    Identification and analysis of novel functional sites in human GD3-synthase (2008), Biochem. Biophys. Res. Commun., 370, 67-71.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.8 expression in CHO-K1 cells. Secretion of mouse interleukin-2 signal sequence and transmembrane domain truncated human GD3-synthase cDNA amino-acid residues 49-356 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.3.8 secondary structure alignments between Campylobacter jejunii sialyltransferase CstII and human GD3-synthase. In the human enzyme, the side chain on residue N188 has a strong hydrogen bond with the carboxyl group on the sialic acid group of the donor substrate. Residue P189 has no interaction with the donor. The distance between S190 and the donor substrate is 4.4 A, this residue might weakly interact with the donor. R272 is 8.8 A away from the donor, suggesting that this residue has no function on donor substrate binding Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.3.8 N188D mutation in a conserved residue identified by structure alignments between Campylobacter jejunii sialyltransferase CstII and human GD3-synthase, 6fold decrease in ratio Km to Vmax Homo sapiens
2.4.3.8 R272A mutation in a conserved residue identified by structure alignments between Campylobacter jejunii sialyltransferase CstII and human GD3-synthase, 4fold decrease in ratio Km to Vmax Homo sapiens
2.4.3.8 S190A mutation in a conserved residue identified by structure alignments between Campylobacter jejunii sialyltransferase CstII and human GD3-synthase, 4fold decrease in ratio Km to Vmax Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.8 0.081
-
 CMP-N-acetylneuraminate mutant R272A, pH 6.5, 37°C Homo sapiens
2.4.3.8 0.083
-
 ganglioside GM3 wild-type, pH 6.5, 37°C Homo sapiens
2.4.3.8 0.087
-
 ganglioside GM3 mutant N188D, pH 6.5, 37°C Homo sapiens
2.4.3.8 0.088
-
 CMP-N-acetylneuraminate wild-type, pH 6.5, 37°C Homo sapiens
2.4.3.8 0.11
-
 CMP-N-acetylneuraminate mutant S190A, pH 6.5, 37°C Homo sapiens
2.4.3.8 0.184
-
 ganglioside GM3 mutant S190A, pH 6.5, 37°C Homo sapiens
2.4.3.8 0.22
-
 CMP-N-acetylneuraminate mutant N188D, pH 6.5, 37°C Homo sapiens
2.4.3.8 0.979
-
 ganglioside GM3 mutant R272A, pH 6.5, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.8 Homo sapiens Q92185
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.8 CMP-N-acetylneuraminate + ganglioside GM3
-
 Homo sapiens CMP + ?
-
 ?