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Literature summary extracted from

  • Park, C.G.; Lee, H.S.; Lee, H.Y.; Park, C.S.; Choi, O.H.
    Cloning and characterization of rat sphingosine kinase 1 with an N-terminal extension (2007), Biochem. Biophys. Res. Commun., 364, 702-707.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.91 cloning and characterization of an isoform of rat SK1 with an N-terminal extension (long-rSK1). When the long-rSK1 is transfected in COS-7 cells, SK activity is 53fold increased. Substrate specificity and dependency on divalent cations of long-rSK1 are similar to those of rSK1 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.91 EDTA
-
 Rattus norvegicus
2.7.1.91 N,N-dimethylsphingosine competitive inhibition with D-erythro-sphingosine as substrate Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.91 Ca2+ both isoforms (longrSK1 and rSK1) require divalent cations for their activity, both enzymes show the highest activity with Mg2+, followed by Mn2+ and Ca2+ Rattus norvegicus
2.7.1.91 Mg2+ both isoforms (longrSK1 and rSK1) require divalent cations for their activity, both enzymes show the highest activity with Mg2+, followed by Mn2+ and Ca2+ Rattus norvegicus
2.7.1.91 Mn2+ both isoforms (longrSK1 and rSK1) require divalent cations for their activity, both enzymes show the highest activity with Mg2+, followed by Mn2+ and Ca2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.91 49800
-
 x * 49800, calculated from sequence Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.91 additional information Rattus norvegicus long-rSK1 is involved in cell signaling ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.91 Rattus norvegicus Q1HGK5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.91 brain expression of long-rSK1 mRNA Rattus norvegicus
-
2.7.1.91 heart expression of long-rSK1 mRNA, no expression of rSK1 mRNA Rattus norvegicus
-
2.7.1.91 kidney expression of long-rSK1 mRNA Rattus norvegicus
-
2.7.1.91 liver expression of long-rSK1 mRNA, no expression of rSK1 mRNA Rattus norvegicus
-
2.7.1.91 lung expression of long-rSK1 mRNA Rattus norvegicus
-
2.7.1.91 spleen expression of long-rSK1 mRNA Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.91 ATP + D-erythro-dihydrosphingosine the activity of longrSK1 towards dihydrosphingosine is about 3.6fold higher than that of rSK1 Rattus norvegicus ADP + D-erythro-dihydrosphingosine 1-phosphate
-
 ?
2.7.1.91 ATP + D-erythro-sphingosine
-
 Rattus norvegicus ADP + D-erythro-sphingosine 1-phosphate
-
 ?
2.7.1.91 additional information long-rSK1 is involved in cell signaling Rattus norvegicus ?
-
 ?
2.7.1.91 additional information no phosphorylation of with threo-dihydrosphingosine, N,N-dimethylsphingosine and N-acetyl-D-sphingosine Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.91 ? x * 49800, calculated from sequence Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.91 SK1
-
 Rattus norvegicus
2.7.1.91 sphingosine kinase 1
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.91 0.00033
-
 N,N-dimethylsphingosine rSK1 Rattus norvegicus
2.7.1.91 0.0058
-
 N,N-dimethylsphingosine long-rSK1 Rattus norvegicus