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Literature summary extracted from

  • Park, S.W.; Song, T.; Kim, S.Y.; Kim, E.; Oh, J.I.; Eom, C.Y.; Kim, Y.M.
    Carbon monoxide dehydrogenase in mycobacteria possesses a nitric oxide dehydrogenase activity (2007), Biochem. Biophys. Res. Commun., 362, 449-453.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.4 sodium nitroprusside 5 mM sodium nitroprusside induces CO-DH gene expression after 20 min exposure Mycobacterium tuberculosis
1.2.7.4 sodium nitroprusside 5 mM sodium nitroprusside induces CO-DH gene expression after 20 min exposure Mycobacterium sp.
1.2.7.4 sodium nitroprusside 5 mM sodium nitroprusside induces CO-DH gene expression after 20 min exposure Mycolicibacterium vaccae
1.2.7.4 sodium nitroprusside activity increases in the presence of 5 mM sodium nitroprusside during 20 min incubation Mycobacterium tuberculosis
1.2.7.4 sodium nitroprusside activity increases in the presence of 5 mM sodium nitroprusside during 20 min incubation Mycobacterium sp.
1.2.7.4 sodium nitroprusside activity increases in the presence of 5 mM sodium nitroprusside during 20 min incubation Mycolicibacterium vaccae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.7.4 expressed in Escherichia coli DH5 alpha cells Mycobacterium tuberculosis
1.2.7.4 expressed in Escherichia coli DH5 alpha cells Mycobacterium sp.
1.2.7.4 expressed in Escherichia coli DH5 alpha cells Mycolicibacterium vaccae

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Mycobacterium sp.
-
-
-
1.2.7.4 Mycobacterium sp. DSM 3803 / JC1
-
-
-
1.2.7.4 Mycobacterium tuberculosis
-
-
-
1.2.7.4 Mycobacterium tuberculosis H37Ra / ATCC 25177
-
-
-
1.2.7.4 Mycobacterium tuberculosis TMC 326 / H37Ra-INH-R / ATCC 35835
-
-
-
1.2.7.4 Mycolicibacterium vaccae
-
 strain ATCC 15483
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.4 0.006
-
 at 37°C Mycobacterium sp.
1.2.7.4 0.0099
-
 at 37°C Mycobacterium tuberculosis
1.2.7.4 0.0175
-
 at 37°C Mycolicibacterium vaccae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 Mycobacterium tuberculosis CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 ?
1.2.7.4 CO + H2O + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 Mycobacterium sp. CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 ?
1.2.7.4 CO + H2O + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 Mycolicibacterium vaccae CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 ?
1.2.7.4 CO + H2O + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 Mycobacterium tuberculosis H37Ra / ATCC 25177 CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 ?
1.2.7.4 CO + H2O + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 Mycobacterium sp. DSM 3803 / JC1 CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 ?
1.2.7.4 CO + H2O + 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 Mycobacterium tuberculosis TMC 326 / H37Ra-INH-R / ATCC 35835 CO2 + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium chloride
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.7.4 CO dehydrogenase
-
 Mycobacterium tuberculosis
1.2.7.4 CO dehydrogenase
-
 Mycobacterium sp.
1.2.7.4 CO dehydrogenase
-
 Mycolicibacterium vaccae
1.2.7.4 CO-DH
-
 Mycobacterium tuberculosis
1.2.7.4 CO-DH
-
 Mycobacterium sp.
1.2.7.4 CO-DH
-
 Mycolicibacterium vaccae
1.2.7.4 CO-DH possesses also nitric oxid dehydrogenase activity in mycobacteria Mycobacterium tuberculosis
1.2.7.4 CO-DH possesses also nitric oxid dehydrogenase activity in mycobacteria Mycobacterium sp.
1.2.7.4 CO-DH possesses also nitric oxid dehydrogenase activity in mycobacteria Mycolicibacterium vaccae