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Literature summary extracted from

  • Yu, H.N.; Song, E.K.; Yoo, S.M.; Lee, Y.R.; Han, M.K.; Yim, C.Y.; Kwak, J.Y.; Kim, J.S.
    Activation of NUDT5, an ADP-ribose pyrophosphatase, by nitric oxide-mediated ADP-ribosylation (2007), Biochem. Biophys. Res. Commun., 354, 764-768.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.96 nitric oxide nitric oxide stimulates nonenzymatic ADP-ribosylation of NUDT5 at cysteine residues using ADP-ribose and consequently activates its enzyme activity Homo sapiens
3.6.1.13 nitric oxide NO stimulates non-enzymatic ADP-ribosylation, at cysteine residues in the presence of reductant, of NUDT5 using ADP-ribose and consequently activates its ADPRase activity. ADPRase activity in J774 macrophage cells is increased by the treatment with SNP, an exogenous NO generator, or TNF-alpha/IFN-gamma, endogenous NO inducers. NO has a regulatory role, overview Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.13 gene NUDT5, expression in murine J774A.1 macrophage cells, expression of His6-tagged NUDT5 in Escherichia coli strain BL21 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.96 N(G)-nitro-L-arginine methyl ester
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.13 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.96 40000
-
 x * 40000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.96 ATP + D-ribose 5-phosphate Homo sapiens
-
 diphosphate + ADP-D-ribose
-
 ?
3.6.1.13 ADP-ribose + H2O Homo sapiens
-
 AMP + D-ribose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.96 Homo sapiens
-
-
-
3.6.1.13 Homo sapiens
-
 gene NUDT5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.96 Ni-NTA column chromatography Homo sapiens
3.6.1.13 recombinant His6-tagged NUDT5 from Escherichia coli strain BL21 by nickel affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.96 J-774 cell
-
 Homo sapiens
-
2.7.7.96 macrophage
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.96 ATP + D-ribose 5-phosphate
-
 Homo sapiens diphosphate + ADP-D-ribose
-
 ?
3.6.1.13 1,Nepsilon-etheno-ADP-ribose + H2O fluorogenic substrate Homo sapiens 1,Nepsilon-etheno-AMP-ribose + phosphate the product is converted to fluorescent 1,Nepsilon-etheno-adenosine by alkaline phosphatase for detection ?
3.6.1.13 ADP-ribose + H2O
-
 Homo sapiens AMP + D-ribose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.96 ? x * 40000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.7.96 ADP-ribose pyrophosphatase
-
 Homo sapiens
2.7.7.96 ADPRase
-
 Homo sapiens
2.7.7.96 NUDT5
-
 Homo sapiens
3.6.1.13 ADP-ribose pyrophosphatase
-
 Homo sapiens
3.6.1.13 ADPR pyrophosphatase
-
 Homo sapiens
3.6.1.13 ADPRase
-
 Homo sapiens
3.6.1.13 ADPRase-I
-
 Homo sapiens
3.6.1.13 More the enzyme is a member of a superfamily of Nudix hydrolases Homo sapiens
3.6.1.13 NUDT5
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.13 37
-
 assay at Homo sapiens