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Literature summary extracted from

  • Wen, G.; Chen, C.; Luo, X.; Wang, Y.; Zhang, C.; Pan, Z.
    Identification and characterization of the NTPase activity of classical swine fever virus (CSFV) nonstructural protein 3 (NS3) expressed in bacteria (2007), Arch. Virol., 152, 1565-1573.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.15 Poly(U) the activation by poly(U) is inhibited by K+ Classical swine fever virus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.15 DNA and amino acid sequence determination and analysis, functional expression of His-tagged full-length viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3 in Escherichia coli Classical swine fever virus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.15 A354V site-directed mutagenesis of the motif III residue does not affect the enzyme activity and kinetics Classical swine fever virus
3.6.1.15 K232A site-directed mutagenesis of the conserved motif I residue leads to inactivation of the mutant Classical swine fever virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.15 EDTA
-
Classical swine fever virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.15 Mg2+ required, activates Classical swine fever virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.15 60000
-
x * 60000, recombinant full-length NS3 protein, SDS-PAGE Classical swine fever virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.15 ATP + H2O Classical swine fever virus
-
ADP + phosphate
-
?
3.6.1.15 ATP + H2O Classical swine fever virus CSFV
-
ADP + phosphate
-
?
3.6.1.15 CTP + H2O Classical swine fever virus
-
CDP + phosphate
-
?
3.6.1.15 dATP + H2O Classical swine fever virus
-
dADP + phosphate
-
?
3.6.1.15 dCTP + H2O Classical swine fever virus
-
dCDP + phosphate
-
?
3.6.1.15 dGTP + H2O Classical swine fever virus
-
dGDP + phosphate
-
?
3.6.1.15 dUTP + H2O Classical swine fever virus
-
dUDP + phosphate
-
?
3.6.1.15 GTP + H2O Classical swine fever virus
-
GDP + phosphate
-
?
3.6.1.15 GTP + H2O Classical swine fever virus CSFV
-
GDP + phosphate
-
?
3.6.1.15 additional information Classical swine fever virus the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication ?
-
?
3.6.1.15 additional information Classical swine fever virus CSFV the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication ?
-
?
3.6.1.15 UTP + H2O Classical swine fever virus
-
UDP + phosphate
-
?
3.6.1.15 UTP + H2O Classical swine fever virus CSFV
-
UDP + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.15 Classical swine fever virus
-
CSFV
-
3.6.1.15 Classical swine fever virus CSFV
-
CSFV
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.15 recombinant His-tagged full-length viral NS3 protein and N-terminal truncated version ntNS3 from Escherichia coli by nickel affinity chromatography and dialysis Classical swine fever virus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.15 additional information
-
-
Classical swine fever virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.15 ATP + H2O
-
Classical swine fever virus ADP + phosphate
-
?
3.6.1.15 ATP + H2O
-
Classical swine fever virus CSFV ADP + phosphate
-
?
3.6.1.15 CTP + H2O
-
Classical swine fever virus CDP + phosphate
-
?
3.6.1.15 dATP + H2O
-
Classical swine fever virus dADP + phosphate
-
?
3.6.1.15 dCTP + H2O
-
Classical swine fever virus dCDP + phosphate
-
?
3.6.1.15 dGTP + H2O
-
Classical swine fever virus dGDP + phosphate
-
?
3.6.1.15 dUTP + H2O
-
Classical swine fever virus dUDP + phosphate
-
?
3.6.1.15 GTP + H2O
-
Classical swine fever virus GDP + phosphate
-
?
3.6.1.15 GTP + H2O
-
Classical swine fever virus CSFV GDP + phosphate
-
?
3.6.1.15 additional information the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication Classical swine fever virus ?
-
?
3.6.1.15 additional information the conserved motif I, GXGK232T, is required for NTPase activity of the viral NS3 protein, while motif III is not Classical swine fever virus ?
-
?
3.6.1.15 additional information the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication Classical swine fever virus CSFV ?
-
?
3.6.1.15 additional information the conserved motif I, GXGK232T, is required for NTPase activity of the viral NS3 protein, while motif III is not Classical swine fever virus CSFV ?
-
?
3.6.1.15 UTP + H2O
-
Classical swine fever virus UDP + phosphate
-
?
3.6.1.15 UTP + H2O
-
Classical swine fever virus CSFV UDP + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.6.1.15 ? x * 60000, recombinant full-length NS3 protein, SDS-PAGE Classical swine fever virus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.15 More the enzyme is a nonstructural protein 3, NS3 of family Flaviviridae members Classical swine fever virus
3.6.1.15 NS3 protein
-
Classical swine fever virus
3.6.1.15 NS3FL
-
Classical swine fever virus
3.6.1.15 NTPase
-
Classical swine fever virus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.15 37
-
assay at Classical swine fever virus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.15 82
-
dUTP pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) Classical swine fever virus
3.6.1.15 114
-
UTP pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) Classical swine fever virus
3.6.1.15 135
-
dCTP pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) Classical swine fever virus
3.6.1.15 136
-
dGTP pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) Classical swine fever virus
3.6.1.15 155
-
ATP pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) Classical swine fever virus
3.6.1.15 208
-
dATP pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) Classical swine fever virus
3.6.1.15 220
-
CTP pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) Classical swine fever virus
3.6.1.15 400
-
GTP pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U) Classical swine fever virus
3.6.1.15 1875
-
dUTP pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) Classical swine fever virus
3.6.1.15 2162
-
UTP pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) Classical swine fever virus
3.6.1.15 2286
-
CTP pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) Classical swine fever virus
3.6.1.15 2500
-
dGTP pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) Classical swine fever virus
3.6.1.15 2963
-
GTP pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) Classical swine fever virus
3.6.1.15 3150
-
dATP pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) Classical swine fever virus
3.6.1.15 4384
-
ATP pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) Classical swine fever virus
3.6.1.15 5096
-
dCTP pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U) Classical swine fever virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.15 6 9 broad range optimum, pH profile Classical swine fever virus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.15 5.5 9 pH profile Classical swine fever virus