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Literature summary extracted from

  • Lee, M.J.; Huang, H.; Lin, W.; Yang, R.R.; Liu, C.L.; Huang, C.Y.
    Activation of Helicobacter pylori inorganic pyrophosphatase and the importance of Cys16 in thermostability, enzyme activation and quaternary structure (2007), Arch. Microbiol., 188, 473-482.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.1 GSH activates up to 4 mM, and inhibits at higher concentrations Helicobacter pylori
3.6.1.1 NaBH4 activates at above 12 mM Helicobacter pylori

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.1 C16S site-directed mutagenesis, the mutant shows a loss of 50% activity and a reduction in sensitivity to reductants and oxidized glutathione compared to the wild-type enzyme. In addition, the replacement causes a considerable disruption in thermostability, C16S substitution destabilizes PPase through impairing trimer-trimer interactions Helicobacter pylori
3.6.1.1 D69G site-directed mutagenesis, inactive mutant Helicobacter pylori
3.6.1.1 K103A site-directed mutagenesis, inactive mutant Helicobacter pylori
3.6.1.1 Y140F site-directed mutagenesis, the mutant shows 78% reduced activity compared to the wild-type enzyme Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.1 GSH activates up to 4 mM, and inhibits at higher concentrations Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.1 additional information
-
additional information effects of cysteine, homocysteine, and NaBH4 on kinetics of wild-type and mutant C16S enzymes, overview Helicobacter pylori
3.6.1.1 0.083
-
diphosphate pH 8.0, 37°C, recombinant wild-type enzyme Helicobacter pylori
3.6.1.1 0.098
-
diphosphate pH 8.0, 37°C, recombinant mutant C16S Helicobacter pylori
3.6.1.1 0.173
-
diphosphate pH 8.0, 37°C, recombinant mutant Y140F Helicobacter pylori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 cytosol
-
Helicobacter pylori 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Mg2+ dependent on, other divalent cations substituted poorly for Mg2+ Helicobacter pylori
3.6.1.1 additional information no effect by Ca2+, Cd2+, and Ni2+ Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.1 20000
-
6 * 20000 Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Helicobacter pylori
-
2 phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Helicobacter pylori P56153
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
Helicobacter pylori 2 phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 hexamer 6 * 20000 Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 inorganic pyrophosphatase
-
Helicobacter pylori
3.6.1.1 More Helicobacter pylori is a family I PPase Helicobacter pylori
3.6.1.1 PPase
-
Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.1 37
-
assay at Helicobacter pylori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.1 additional information
-
residue Cys116 is involved in thermostability Helicobacter pylori
3.6.1.1 37
-
15 min, the mutant enzyme loses 70% activity in absence of Mg2+, half-life is 4.2 min Helicobacter pylori
3.6.1.1 74.3
-
T1/2 of the wild-type enzyme in absence of Mg2+ Helicobacter pylori
3.6.1.1 77.6
-
T1/2 of the wild-type enzyme in presence of Mg2+ Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.1 78.4
-
diphosphate pH 8.0, 37°C, recombinant mutant Y140F Helicobacter pylori
3.6.1.1 173.1
-
diphosphate pH 8.0, 37°C, recombinant mutant C16S Helicobacter pylori
3.6.1.1 343.9
-
diphosphate pH 8.0, 37°C, recombinant wild-type enzyme Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.1 8
-
-
Helicobacter pylori

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.6.1.1 17.2
-
pH 8.0, 37°C, recombinant wild-type enzyme Helicobacter pylori GSH