EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.1 | GSH | activates up to 4 mM, and inhibits at higher concentrations | Helicobacter pylori | |
3.6.1.1 | NaBH4 | activates at above 12 mM | Helicobacter pylori |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.1 | C16S | site-directed mutagenesis, the mutant shows a loss of 50% activity and a reduction in sensitivity to reductants and oxidized glutathione compared to the wild-type enzyme. In addition, the replacement causes a considerable disruption in thermostability, C16S substitution destabilizes PPase through impairing trimer-trimer interactions | Helicobacter pylori |
3.6.1.1 | D69G | site-directed mutagenesis, inactive mutant | Helicobacter pylori |
3.6.1.1 | K103A | site-directed mutagenesis, inactive mutant | Helicobacter pylori |
3.6.1.1 | Y140F | site-directed mutagenesis, the mutant shows 78% reduced activity compared to the wild-type enzyme | Helicobacter pylori |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.1 | GSH | activates up to 4 mM, and inhibits at higher concentrations | Helicobacter pylori |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.1 | additional information | - |
additional information | effects of cysteine, homocysteine, and NaBH4 on kinetics of wild-type and mutant C16S enzymes, overview | Helicobacter pylori | |
3.6.1.1 | 0.083 | - |
diphosphate | pH 8.0, 37°C, recombinant wild-type enzyme | Helicobacter pylori | |
3.6.1.1 | 0.098 | - |
diphosphate | pH 8.0, 37°C, recombinant mutant C16S | Helicobacter pylori | |
3.6.1.1 | 0.173 | - |
diphosphate | pH 8.0, 37°C, recombinant mutant Y140F | Helicobacter pylori |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.1.1 | cytosol | - |
Helicobacter pylori | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.1 | Mg2+ | dependent on, other divalent cations substituted poorly for Mg2+ | Helicobacter pylori | |
3.6.1.1 | additional information | no effect by Ca2+, Cd2+, and Ni2+ | Helicobacter pylori |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | 20000 | - |
6 * 20000 | Helicobacter pylori |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.1 | diphosphate + H2O | Helicobacter pylori | - |
2 phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.1 | Helicobacter pylori | P56153 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.1 | diphosphate + H2O | - |
Helicobacter pylori | 2 phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.1 | hexamer | 6 * 20000 | Helicobacter pylori |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.1 | inorganic pyrophosphatase | - |
Helicobacter pylori |
3.6.1.1 | More | Helicobacter pylori is a family I PPase | Helicobacter pylori |
3.6.1.1 | PPase | - |
Helicobacter pylori |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | 37 | - |
assay at | Helicobacter pylori |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | additional information | - |
residue Cys116 is involved in thermostability | Helicobacter pylori |
3.6.1.1 | 37 | - |
15 min, the mutant enzyme loses 70% activity in absence of Mg2+, half-life is 4.2 min | Helicobacter pylori |
3.6.1.1 | 74.3 | - |
T1/2 of the wild-type enzyme in absence of Mg2+ | Helicobacter pylori |
3.6.1.1 | 77.6 | - |
T1/2 of the wild-type enzyme in presence of Mg2+ | Helicobacter pylori |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.1 | 78.4 | - |
diphosphate | pH 8.0, 37°C, recombinant mutant Y140F | Helicobacter pylori | |
3.6.1.1 | 173.1 | - |
diphosphate | pH 8.0, 37°C, recombinant mutant C16S | Helicobacter pylori | |
3.6.1.1 | 343.9 | - |
diphosphate | pH 8.0, 37°C, recombinant wild-type enzyme | Helicobacter pylori |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | 8 | - |
- |
Helicobacter pylori |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.6.1.1 | 17.2 | - |
pH 8.0, 37°C, recombinant wild-type enzyme | Helicobacter pylori | GSH |