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Literature summary extracted from

  • Bertea, C.M.; Schalk, M.; Karp, F.; Maffei, M.; Croteau, R.
    Demonstration that menthofuran synthase of mint (Mentha) is a cytochrome P450 monooxygenase: cloning, functional expression, and characterization of the responsible gene (2001), Arch. Biochem. Biophys., 390, 279-286.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.143 DNA and amino acid sequence determinationand analysis, functional expression in Saccharomyces cerevisiae strains WAT11U and WAT21U, which constitutively express two different isoforms of the Arabidposis thaliana NADPH-cytochrome P450 reductase, and in Escherichia coli strain JM109 Mentha pulegium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.143 microsome
-
Mentha x piperita
-
-
1.14.14.143 microsome
-
Mentha pulegium
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.143 Fe2+
-
Mentha x piperita
1.14.14.143 Fe2+
-
Mentha pulegium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.143 55360
-
x * 55360, sequence calculation Mentha pulegium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.143 (+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2 Mentha x piperita
-
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.143 (+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2 Mentha pulegium
-
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.143 Mentha pulegium
-
-
-
1.14.14.143 Mentha x piperita
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.143 leaf
-
Mentha x piperita
-
1.14.14.143 leaf
-
Mentha pulegium
-
1.14.14.143 plant oil gland secretory cells Mentha x piperita
-
1.14.14.143 plant oil gland secretory cells Mentha pulegium
-
1.14.14.143 secretory cell
-
Mentha x piperita
-
1.14.14.143 secretory cell
-
Mentha pulegium
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.143 (+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
-
Mentha x piperita (+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.143 (+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
-
Mentha pulegium (+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.143 (+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation of the syn-methyl group of (+)-pulegone, spontaneous intramolecular cyclization to the hemiketal, and dehydration to the furan Mentha x piperita (+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.143 (+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation of the syn-methyl group of (+)-pulegone, spontaneous intramolecular cyclization to the hemiketal, and dehydration to the furan Mentha pulegium (+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O GC-MS product analysis ?

Subunits

EC Number Subunits Comment Organism
1.14.14.143 ? x * 55360, sequence calculation Mentha pulegium

Synonyms

EC Number Synonyms Comment Organism
1.14.14.143 (+)-pulegone-9-hydroxylase
-
Mentha x piperita
1.14.14.143 (+)-pulegone-9-hydroxylase
-
Mentha pulegium
1.14.14.143 menthofuran synthase
-
Mentha x piperita
1.14.14.143 menthofuran synthase
-
Mentha pulegium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.143 30
-
assay at Mentha pulegium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.143 7.4
-
assay at Mentha pulegium

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.143 cytochrome P450 a cytochrome P450 monooxygenase Mentha x piperita
1.14.14.143 cytochrome P450 a cytochrome P450 monooxygenase Mentha pulegium
1.14.14.143 FAD
-
Mentha pulegium
1.14.14.143 FMN
-
Mentha pulegium