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Literature summary extracted from

  • Kremer, A.; Li, S.M.
    Potential of a 7-dimethylallyltryptophan synthase as a tool for production of prenylated indole derivatives (2008), Appl. Microbiol. Biotechnol., 79, 951-961.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.34 into pGEM-T and subsequently into pQE60 for expression in Escherichia coli XL1 Blue MRF cells Aspergillus fumigatus
2.5.1.80 expression in Escherichia coli, His-tagged protein Aspergillus fumigatus

General Stability

EC Number General Stability Organism
2.5.1.80 37°C, absence of substrate, 16 h, 80% residual activity Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.34 50000
-
determined by SDS-PAGE Aspergillus fumigatus
2.5.1.34 54000
-
calculated for His6-7-DMATS Aspergillus fumigatus
2.5.1.34 65000
-
determined by gel filtration Aspergillus fumigatus
2.5.1.80 50000
-
1 * 50000, SDS-PAGE, 1 * 54000, calculated, His-tagged recombinant protein Aspergillus fumigatus
2.5.1.80 54000
-
1 * 50000, SDS-PAGE, 1 * 54000, calculated, His-tagged recombinant protein Aspergillus fumigatus
2.5.1.80 65000
-
gel filtration Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.34 Aspergillus fumigatus
-
isoform FgaPT2
-
2.5.1.34 Aspergillus fumigatus Q50EL0
-
-
2.5.1.80 Aspergillus fumigatus Q4WYG3 isoform 7-DMATS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.34 His6-7-DMATS is purified with Ni-NTA agarose Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.34 dimethylallyl diphosphate + 1-methyl-L-tryptophan
-
Aspergillus fumigatus diphosphate + 1-methyl-4-(3-methylbut-2-enyl)-L-tryptophan 0.4% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 11-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 11-methyl-DL-4-(gamma,gamma-dimethylallyl)tryptophan 1.6% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 11-methyl-DL-tryptophan
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + 4-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-DL-tryptophan + ? 0.2% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 5-bromo-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 5-bromo-4-(3-methylbut-2-enyl)-DL-tryptophan 0.2% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 5-fluoro-L-tryptophan
-
Aspergillus fumigatus diphosphate + 5-fluoro-4-(3-methylbut-2-enyl)-L-tryptophan 0.2% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 5-hydroxy-L-tryptophan
-
Aspergillus fumigatus diphosphate + 5-hydroxy-4-(3-methylbut-2-enyl)-L-tryptophan 21.0% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 5-methoxy-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 5-methoxy-4-(3-methylbut-2-enyl)-DL-tryptophan 0.6% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 5-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 5-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan 8.1% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 6-fluoro-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 6-fluoro-4-(3-methylbut-2-enyl)-DL-tryptophan 0.2% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 6-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 6-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan 15.2% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + 7-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 7-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan + ? 16.3% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + D-tryptophan
-
Aspergillus fumigatus diphosphate + D-4-(3-methylbut-2-enyl)-tryptophan 9.7% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + D-tryptophan
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + DL-indole-3-lactic acid
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-DL-indole-3-lactic acid 10.5% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + DL-indole-3-lactic acid
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + indole-3-acetic acid
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-indole-3-acetic acid 0.2% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + indole-3-acetic acid
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + indole-3-butyric acid
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-indole-3-butyric acid 0.2% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + indole-3-butyric acid
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + indole-3-propionic acid
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-indole-3-propionic acid 32.2% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + indole-3-propionic acid
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + indole-3-pyruvic acid
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-indole-3-pyruvic acid 29.4% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + indole-3-pyruvic acid
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + L-abrine
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + L-beta-homotryptophan
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-beta-homotryptophan 51.5% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + L-beta-homotryptophan
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan
-
Aspergillus fumigatus diphosphate + L-4-(3-methylbut-2-enyl)-tryptophan
-
?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan hydroxamate
-
Aspergillus fumigatus diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan hydroxamate 22.4% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan hydroxamate
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan methyl ester
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan methyl ester 7.2% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan methyl ester
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + N-acetyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + N-acetyl-DL-4-(gamma,gamma-dimethylallyl)tryptophan 0.2% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + N-acetyl-DL-tryptophan
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + Nalpha-methyl-L-tryptophan i.e. L-abrine Aspergillus fumigatus diphosphate + Nalpha-methyl-L-4-(gamma,gamma-dimethylallyl)tryptophan 58.0% of the yield with L-tryptophan ?
2.5.1.34 dimethylallyl diphosphate + tryptamin
-
Aspergillus fumigatus ?
-
?
2.5.1.34 dimethylallyl diphosphate + tryptamine
-
Aspergillus fumigatus diphosphate + 4-(3-methylbut-2-enyl)-tryptamine 4.6% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + 1-methyl-L-tryptophan
-
Aspergillus fumigatus diphosphate + 1-methyl-7-(3-methylbut-2-enyl)-L-tryptophan 35.8% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + 11-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 11-methyl-DL-7-(gamma,gamma-dimethylallyl)tryptophan 19.1% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + 4-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 4-methyl-7-(3-methylbut-2-enyl)-DL-tryptophan 89.4% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + 5-bromo-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 5-bromo-7-(3-methylbut-2-enyl)-DL-tryptophan 7.2% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + 5-fluoro-L-tryptophan
-
Aspergillus fumigatus diphosphate + 5-fluoro-7-(3-methylbut-2-enyl)-L-tryptophan 10.7% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + 5-hydroxy-L-tryptophan
-
Aspergillus fumigatus diphosphate + 5-hydroxy-7-(3-methylbut-2-enyl)-L-tryptophan 26.7% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + 5-methoxy-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 5-methoxy-7-(3-methylbut-2-enyl)-DL-tryptophan 13.3% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + 5-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 5-methyl-7-(3-methylbut-2-enyl)-DL-tryptophan 74.8% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + 6-fluoro-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 6-fluoro-7-(3-methylbut-2-enyl)-DL-tryptophan 33.7% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + 6-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 6-methyl-7-(3-methylbut-2-enyl)-DL-tryptophan 19.8% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + 7-methyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-DL-tryptophan + ? 0.3% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + D-tryptophan
-
Aspergillus fumigatus diphosphate + D-7-(3-methylbut-2-enyl)-tryptophan 11.8% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + DL-indole-3-lactic acid
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-DL-indole-3-lactic acid 4.3% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + indole-3-acetic acid
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-indole-3-acetic acid 10.8% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + indole-3-butyric acid
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-indole-3-butyric acid 9.1% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + indole-3-propionic acid
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-indole-3-propionic acid 8.5% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + indole-3-pyruvic acid
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-indole-3-pyruvic acid 4.8% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + L-beta-homotryptophan
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-L-beta-homotryptophan 28.2% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + L-tryptophan
-
Aspergillus fumigatus diphosphate + L-7-(3-methylbut-2-enyl)-tryptophan
-
?
2.5.1.80 dimethylallyl diphosphate + L-tryptophan hydroxamate
-
Aspergillus fumigatus diphosphate + L-7-(gamma,gamma-dimethylallyl)tryptophan hydroxamate 4.6% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + L-tryptophan methyl ester
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan methyl ester 7.2% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + N-acetyl-DL-tryptophan
-
Aspergillus fumigatus diphosphate + N-acetyl-DL-7-(gamma,gamma-dimethylallyl)tryptophan 7.7% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + Nalpha-methyl-L-tryptophan i.e. L-abrine Aspergillus fumigatus diphosphate + Nalpha-methyl-L-7-(gamma,gamma-dimethylallyl)tryptophan 82.2% of the yield with L-tryptophan ?
2.5.1.80 dimethylallyl diphosphate + tryptamine
-
Aspergillus fumigatus diphosphate + 7-(3-methylbut-2-enyl)-tryptamine 7.5% of the yield with L-tryptophan ?

Subunits

EC Number Subunits Comment Organism
2.5.1.34 monomer
-
Aspergillus fumigatus
2.5.1.80 monomer 1 * 50000, SDS-PAGE, 1 * 54000, calculated, His-tagged recombinant protein Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.34 7-dimethylallyltryptophan synthase
-
Aspergillus fumigatus
2.5.1.34 7-DMATS
-
Aspergillus fumigatus
2.5.1.80 7-DMATS
-
Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.34 37
-
enzyme assay Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.34 7.5
-
enzyme assay Aspergillus fumigatus