EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.34 | into pGEM-T and subsequently into pQE60 for expression in Escherichia coli XL1 Blue MRF cells | Aspergillus fumigatus |
2.5.1.80 | expression in Escherichia coli, His-tagged protein | Aspergillus fumigatus |
EC Number | General Stability | Organism |
---|---|---|
2.5.1.80 | 37°C, absence of substrate, 16 h, 80% residual activity | Aspergillus fumigatus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.34 | 50000 | - |
determined by SDS-PAGE | Aspergillus fumigatus |
2.5.1.34 | 54000 | - |
calculated for His6-7-DMATS | Aspergillus fumigatus |
2.5.1.34 | 65000 | - |
determined by gel filtration | Aspergillus fumigatus |
2.5.1.80 | 50000 | - |
1 * 50000, SDS-PAGE, 1 * 54000, calculated, His-tagged recombinant protein | Aspergillus fumigatus |
2.5.1.80 | 54000 | - |
1 * 50000, SDS-PAGE, 1 * 54000, calculated, His-tagged recombinant protein | Aspergillus fumigatus |
2.5.1.80 | 65000 | - |
gel filtration | Aspergillus fumigatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.34 | Aspergillus fumigatus | - |
isoform FgaPT2 | - |
2.5.1.34 | Aspergillus fumigatus | Q50EL0 | - |
- |
2.5.1.80 | Aspergillus fumigatus | Q4WYG3 | isoform 7-DMATS | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.34 | His6-7-DMATS is purified with Ni-NTA agarose | Aspergillus fumigatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.34 | dimethylallyl diphosphate + 1-methyl-L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 1-methyl-4-(3-methylbut-2-enyl)-L-tryptophan | 0.4% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + 11-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 11-methyl-DL-4-(gamma,gamma-dimethylallyl)tryptophan | 1.6% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + 11-methyl-DL-tryptophan | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + 4-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-DL-tryptophan + ? | 0.2% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + 5-bromo-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-bromo-4-(3-methylbut-2-enyl)-DL-tryptophan | 0.2% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + 5-fluoro-L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-fluoro-4-(3-methylbut-2-enyl)-L-tryptophan | 0.2% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + 5-hydroxy-L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-hydroxy-4-(3-methylbut-2-enyl)-L-tryptophan | 21.0% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + 5-methoxy-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-methoxy-4-(3-methylbut-2-enyl)-DL-tryptophan | 0.6% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + 5-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan | 8.1% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + 6-fluoro-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 6-fluoro-4-(3-methylbut-2-enyl)-DL-tryptophan | 0.2% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + 6-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 6-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan | 15.2% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + 7-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 7-methyl-4-(3-methylbut-2-enyl)-DL-tryptophan + ? | 16.3% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + D-tryptophan | - |
Aspergillus fumigatus | diphosphate + D-4-(3-methylbut-2-enyl)-tryptophan | 9.7% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + D-tryptophan | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + DL-indole-3-lactic acid | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-DL-indole-3-lactic acid | 10.5% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + DL-indole-3-lactic acid | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + indole-3-acetic acid | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-indole-3-acetic acid | 0.2% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + indole-3-acetic acid | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + indole-3-butyric acid | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-indole-3-butyric acid | 0.2% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + indole-3-butyric acid | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + indole-3-propionic acid | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-indole-3-propionic acid | 32.2% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + indole-3-propionic acid | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + indole-3-pyruvic acid | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-indole-3-pyruvic acid | 29.4% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + indole-3-pyruvic acid | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + L-abrine | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + L-beta-homotryptophan | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-L-beta-homotryptophan | 51.5% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + L-beta-homotryptophan | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus fumigatus | diphosphate + L-4-(3-methylbut-2-enyl)-tryptophan | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + L-tryptophan hydroxamate | - |
Aspergillus fumigatus | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan hydroxamate | 22.4% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + L-tryptophan hydroxamate | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + L-tryptophan methyl ester | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan methyl ester | 7.2% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + L-tryptophan methyl ester | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + N-acetyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + N-acetyl-DL-4-(gamma,gamma-dimethylallyl)tryptophan | 0.2% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + N-acetyl-DL-tryptophan | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + Nalpha-methyl-L-tryptophan | i.e. L-abrine | Aspergillus fumigatus | diphosphate + Nalpha-methyl-L-4-(gamma,gamma-dimethylallyl)tryptophan | 58.0% of the yield with L-tryptophan | ? | |
2.5.1.34 | dimethylallyl diphosphate + tryptamin | - |
Aspergillus fumigatus | ? | - |
? | |
2.5.1.34 | dimethylallyl diphosphate + tryptamine | - |
Aspergillus fumigatus | diphosphate + 4-(3-methylbut-2-enyl)-tryptamine | 4.6% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + 1-methyl-L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 1-methyl-7-(3-methylbut-2-enyl)-L-tryptophan | 35.8% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + 11-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 11-methyl-DL-7-(gamma,gamma-dimethylallyl)tryptophan | 19.1% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + 4-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 4-methyl-7-(3-methylbut-2-enyl)-DL-tryptophan | 89.4% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + 5-bromo-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-bromo-7-(3-methylbut-2-enyl)-DL-tryptophan | 7.2% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + 5-fluoro-L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-fluoro-7-(3-methylbut-2-enyl)-L-tryptophan | 10.7% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + 5-hydroxy-L-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-hydroxy-7-(3-methylbut-2-enyl)-L-tryptophan | 26.7% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + 5-methoxy-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-methoxy-7-(3-methylbut-2-enyl)-DL-tryptophan | 13.3% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + 5-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 5-methyl-7-(3-methylbut-2-enyl)-DL-tryptophan | 74.8% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + 6-fluoro-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 6-fluoro-7-(3-methylbut-2-enyl)-DL-tryptophan | 33.7% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + 6-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 6-methyl-7-(3-methylbut-2-enyl)-DL-tryptophan | 19.8% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + 7-methyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-DL-tryptophan + ? | 0.3% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + D-tryptophan | - |
Aspergillus fumigatus | diphosphate + D-7-(3-methylbut-2-enyl)-tryptophan | 11.8% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + DL-indole-3-lactic acid | - |
Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-DL-indole-3-lactic acid | 4.3% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + indole-3-acetic acid | - |
Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-indole-3-acetic acid | 10.8% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + indole-3-butyric acid | - |
Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-indole-3-butyric acid | 9.1% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + indole-3-propionic acid | - |
Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-indole-3-propionic acid | 8.5% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + indole-3-pyruvic acid | - |
Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-indole-3-pyruvic acid | 4.8% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + L-beta-homotryptophan | - |
Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-L-beta-homotryptophan | 28.2% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + L-tryptophan | - |
Aspergillus fumigatus | diphosphate + L-7-(3-methylbut-2-enyl)-tryptophan | - |
? | |
2.5.1.80 | dimethylallyl diphosphate + L-tryptophan hydroxamate | - |
Aspergillus fumigatus | diphosphate + L-7-(gamma,gamma-dimethylallyl)tryptophan hydroxamate | 4.6% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + L-tryptophan methyl ester | - |
Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan methyl ester | 7.2% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + N-acetyl-DL-tryptophan | - |
Aspergillus fumigatus | diphosphate + N-acetyl-DL-7-(gamma,gamma-dimethylallyl)tryptophan | 7.7% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + Nalpha-methyl-L-tryptophan | i.e. L-abrine | Aspergillus fumigatus | diphosphate + Nalpha-methyl-L-7-(gamma,gamma-dimethylallyl)tryptophan | 82.2% of the yield with L-tryptophan | ? | |
2.5.1.80 | dimethylallyl diphosphate + tryptamine | - |
Aspergillus fumigatus | diphosphate + 7-(3-methylbut-2-enyl)-tryptamine | 7.5% of the yield with L-tryptophan | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.34 | monomer | - |
Aspergillus fumigatus |
2.5.1.80 | monomer | 1 * 50000, SDS-PAGE, 1 * 54000, calculated, His-tagged recombinant protein | Aspergillus fumigatus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.34 | 7-dimethylallyltryptophan synthase | - |
Aspergillus fumigatus |
2.5.1.34 | 7-DMATS | - |
Aspergillus fumigatus |
2.5.1.80 | 7-DMATS | - |
Aspergillus fumigatus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.34 | 37 | - |
enzyme assay | Aspergillus fumigatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.34 | 7.5 | - |
enzyme assay | Aspergillus fumigatus |