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Literature summary extracted from

  • Sutay Kocabas, D.; Bakir, U.; Phillips, S.E.; McPherson, M.J.; Ogel, Z.B.
    Purification, characterization, and identification of a novel bifunctional catalase-phenol oxidase from Scytalidium thermophilum (2008), Appl. Microbiol. Biotechnol., 79, 407-415.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 p-coumaric acid 14% residual activity at 0.5 M Mycothermus thermophilus
1.14.18.1 Salicylhydroxamic acid 36% residual activity at 0.5 M Mycothermus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.6 extracellular
-
 Mycothermus thermophilus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 80000
-
 4 * 80000, SDS-PAGE Mycothermus thermophilus
1.11.1.6 320000
-
 PAGE Mycothermus thermophilus
1.14.18.1 80000
-
 4 * 80000 Mycothermus thermophilus
1.14.18.1 320000
-
 SDS-PAGE Mycothermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Aspergillus niger
-
-
-
1.10.3.1 Bos taurus
-
-
-
1.10.3.1 Homo sapiens
-
-
-
1.11.1.6 Mycothermus thermophilus
-
 bifunctional catalase-phenol oxidase
-
1.14.18.1 Mycothermus thermophilus
-
 type culture Humicola insolens, ATCC 16454
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6
-
 Mycothermus thermophilus
1.14.18.1 HiPrep 16/60 Q XL column chromatography and Sephacryl S-100 gel filtration Mycothermus thermophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 erythrocyte
-
 Homo sapiens
-
1.10.3.1 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 444.4
-
 60°C, pH 7.0 Mycothermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 2 catechol + O2 the highest oxidase activity is observed against catechol Homo sapiens 2 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.1 2 catechol + O2 the highest oxidase activity is observed against catechol Bos taurus 2 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.1 2 catechol + O2 the highest oxidase activity is observed against catechol Aspergillus niger 2 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.1 caffeic acid + O2
-
 Homo sapiens caffeoyl quinone + H2O
-
 ?
1.10.3.1 caffeic acid + O2
-
 Bos taurus caffeoyl quinone + H2O
-
 ?
1.10.3.1 caffeic acid + O2
-
 Aspergillus niger caffeoyl quinone + H2O
-
 ?
1.10.3.1 L-Dopa + O2
-
 Homo sapiens L-dopaquinone + H2O
-
 ?
1.10.3.1 L-Dopa + O2
-
 Bos taurus L-dopaquinone + H2O
-
 ?
1.10.3.1 L-Dopa + O2
-
 Aspergillus niger L-dopaquinone + H2O
-
 ?
1.10.3.1 additional information no activity is detected against L-tyrosine and common laccase substrates such as 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and syringaldazine with the exception of weak activity with p-hydroquinone Homo sapiens ?
-
 ?
1.10.3.1 additional information no activity is detected against L-tyrosine and common laccase substrates such as 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and syringaldazine with the exception of weak activity with p-hydroquinone Bos taurus ?
-
 ?
1.10.3.1 additional information no activity is detected against L-tyrosine and common laccase substrates such as 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and syringaldazine with the exception of weak activity with p-hydroquinone Aspergillus niger ?
-
 ?
1.10.3.1 p-hydroquinone + O2
-
 Homo sapiens ?
-
 ?
1.10.3.1 p-hydroquinone + O2
-
 Bos taurus ?
-
 ?
1.10.3.1 p-hydroquinone + O2
-
 Aspergillus niger ?
-
 ?
1.11.1.6 H2O2
-
 Mycothermus thermophilus O2 + H2O
-
 ?
1.14.18.1 2 catechol + O2 the highest oxidase activity is observed against catechol Mycothermus thermophilus 2 1,2-benzoquinone + 2 H2O
-
 ?
1.14.18.1 caffeic acid + O2 50% activity compared to catechol Mycothermus thermophilus caffeoyl quinone + H2O
-
 ?
1.14.18.1 L-Dopa + O2 30% activity compared to catechol Mycothermus thermophilus L-dopaquinone + H2O
-
 ?
1.14.18.1 additional information no activity is detected against L-tyrosine and common laccase substrates such as 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and syringaldazine with the exception of weak activity with p-hydroquinone Mycothermus thermophilus ?
-
 ?
1.14.18.1 p-hydroquinone + O2 2% activity compared to catechol Mycothermus thermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 tetramer 4 * 80000, SDS-PAGE Mycothermus thermophilus
1.14.18.1 homotetramer 4 * 80000 Mycothermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 catalase-phenol oxidase bifunctional enzyme Homo sapiens
1.10.3.1 catalase-phenol oxidase bifunctional enzyme Bos taurus
1.10.3.1 catalase-phenol oxidase bifunctional enzyme Aspergillus niger
1.10.3.1 CATPO
-
 Homo sapiens
1.10.3.1 CATPO
-
 Bos taurus
1.10.3.1 CATPO
-
 Aspergillus niger
1.14.18.1 catalase-phenol oxidase bifunctional enzyme Mycothermus thermophilus
1.14.18.1 CATPO
-
 Mycothermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.6 60
-
 more than 70% of catalase maximum activity within Mycothermus thermophilus
1.14.18.1 60
-
-
 Mycothermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.11.1.6 30 75
-
 Mycothermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.18.1 30 75 relative activity is greater than 70% between 30°C and 75°C for catalase activity, no decrease in activity is observed at 50°C for 2 h, CATPO retains 80% and 45% of its original catalase activity at 60 and 70°C, respectively. Catalase activity is essentially lost after 2 h incubation at 80°C, and at this temperature catalase activity is rapidly reduced with only 30% remaining after 30 min. Mycothermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 7
-
 both catalase and phenol oxidase activities Mycothermus thermophilus
1.14.18.1 7
-
-
 Mycothermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.6 5 9 more than 60% of catalase maximum activity within Mycothermus thermophilus
1.11.1.6 6 8 more than 80% of catalase maximum activity within Mycothermus thermophilus
1.14.18.1 5 9 some ca. 80% of catalase activity is retained between pH 6 and 8, while 80% of phenol oxidase activity is retained between pH 7 and 8 with only ca. 25% activity at pH 6 and ca. 50% at pH 8, retains ca. 80% of the initial activity across the pH range after 24 h incubation Mycothermus thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.6 7
-
 24 h, more than 90% of initial activity Mycothermus thermophilus
1.14.18.1 5.5 9 most stable at pH 7.0, more than 90% of the initial activity remains after 24 h incubation at pH 7, catalase retains more than 60% of its activity at low (pH 5.5) and high (pH 9) values Mycothermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.10.3.1 heme the enzyme contains heme Homo sapiens
1.10.3.1 heme the enzyme contains heme Bos taurus
1.10.3.1 heme the enzyme contains heme Aspergillus niger
1.11.1.6 heme
-
 Mycothermus thermophilus
1.14.18.1 heme the enzyme contains heme Mycothermus thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.6 Mycothermus thermophilus isoelectric focusing
-
 5
1.14.18.1 Mycothermus thermophilus
-
-
 5