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Literature summary extracted from
- The role of glucosamine-6-phosphate deaminase at the early stages of Aspergillus niger growth in a high-citric-acid-yielding medium (2008), Appl. Microbiol. Biotechnol., 78, 613-619.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.99.6 | N-acetylglucosamine 6-phosphate | activator of the enzyme in amination direction, leads to about 30% increased activity at 0.07-0.14 mM, overview | Aspergillus niger |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.99.6 | 1.7 | - |
fructose 6-phosphate | amination reaction, pH 8.0, 30°C | Aspergillus niger | |
| 3.5.99.6 | 5 | - |
glucosamine 6-phosphate | deamination reaction, pH 8.0, 30°C | Aspergillus niger | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.99.6 | additional information | the enzyme is not affected by ammonium ions at 5-20 mM | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.99.6 | glucosamine 6-phosphate + H2O | Aspergillus niger | the main enzyme in Aspergillus niger cells responsible for rapid glucosamine accumulation during the early stages of growth in a high-citric-acid-yielding medium, the enzyme must compete with 6-phosphofructo-1-kinase for the common substrate fructose 6-phosphate in Aspergillus niger cells, role of glucosamine in the pathway regulation, overview | fructose 6-phosphate + NH3 | - |
r | |
| 3.5.99.6 | glucosamine 6-phosphate + H2O | Aspergillus niger A158 | the main enzyme in Aspergillus niger cells responsible for rapid glucosamine accumulation during the early stages of growth in a high-citric-acid-yielding medium, the enzyme must compete with 6-phosphofructo-1-kinase for the common substrate fructose 6-phosphate in Aspergillus niger cells, role of glucosamine in the pathway regulation, overview | fructose 6-phosphate + NH3 | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.99.6 | Aspergillus niger | - |
- |
- |
| 3.5.99.6 | Aspergillus niger A158 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.99.6 | native enzyme by ammonium sulfate fractionation, anion exchange chromatography, and affinity chromatography on a N-aminohexyl-glucosamine-6-phosphate resin folowed by dialysis and ultrafiltration | Aspergillus niger |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.99.6 | mycelium | - |
Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.6 | 0.036 | - |
initial culture, cell homogenate | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.99.6 | glucosamine 6-phosphate + H2O | - |
Aspergillus niger | fructose 6-phosphate + NH3 | - |
r | |
| 3.5.99.6 | glucosamine 6-phosphate + H2O | the main enzyme in Aspergillus niger cells responsible for rapid glucosamine accumulation during the early stages of growth in a high-citric-acid-yielding medium, the enzyme must compete with 6-phosphofructo-1-kinase for the common substrate fructose 6-phosphate in Aspergillus niger cells, role of glucosamine in the pathway regulation, overview | Aspergillus niger | fructose 6-phosphate + NH3 | - |
r | |
| 3.5.99.6 | glucosamine 6-phosphate + H2O | - |
Aspergillus niger A158 | fructose 6-phosphate + NH3 | - |
r | |
| 3.5.99.6 | glucosamine 6-phosphate + H2O | the main enzyme in Aspergillus niger cells responsible for rapid glucosamine accumulation during the early stages of growth in a high-citric-acid-yielding medium, the enzyme must compete with 6-phosphofructo-1-kinase for the common substrate fructose 6-phosphate in Aspergillus niger cells, role of glucosamine in the pathway regulation, overview | Aspergillus niger A158 | fructose 6-phosphate + NH3 | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.99.6 | GlcN6P deaminase | - |
Aspergillus niger |
| 3.5.99.6 | glucosamine-6-phosphate isomerase | - |
Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.6 | 30 | - |
assay at | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.6 | 8.3 | - |
- |
Aspergillus niger |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.6 | 6.8 | 8.3 | 70% of maximal activity at pH 6.8, maximal activity at pH 8.3 | Aspergillus niger |
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