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Literature summary extracted from

  • Kaplan, O.; Vejvoda, V.; Plihal, O.; Pompach, P.; Kavan, D.; Bojarova, P.; Bezouska, K.; Mackova, M.; Cantarella, M.; Jirku, V.; Kren, V.; Martinkova, L.
    Purification and characterization of a nitrilase from Aspergillus niger K10 (2006), Appl. Microbiol. Biotechnol., 73, 567-575.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.5.1 Urea 19% increase of activity at 1% (w/v) Aspergillus niger

General Stability

EC Number General Stability Organism
3.5.5.1 the enzyme stability is markedly improved in the presence of D-sorbitol and xylitol (20% w/v), or myo-inositol (10% w/v) Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 Ag+ complete inhibition at 0.1 mM Aspergillus niger
3.5.5.1 Al2(SO4)3 complete inhibition at 5 mM Aspergillus niger
3.5.5.1 ammonium sulfate 75% residual activity at 800 mM Aspergillus niger
3.5.5.1 Benzamide 66% residual activity at 25 mM Aspergillus niger
3.5.5.1 Ca2+ 96% residual activity at 5 mM Aspergillus niger
3.5.5.1 Co2+ 62% residual activity at 5 mM Aspergillus niger
3.5.5.1 Cr3+ 7% residual activity at 5 mM Aspergillus niger
3.5.5.1 Cu2+ 1% residual activity at 0.1 mM Aspergillus niger
3.5.5.1 dithiothreitol 58% residual activity at 1 mM Aspergillus niger
3.5.5.1 DL-cysteine 97% residual activity at 1% (w/v) Aspergillus niger
3.5.5.1 EDTA 93% residual activity at 15 mM Aspergillus niger
3.5.5.1 Fe2+ 60% residual activity at 5 mM Aspergillus niger
3.5.5.1 Fe3+ 71% residual activity at 5 mM Aspergillus niger
3.5.5.1 H2O2 4% residual activity at 5 mM Aspergillus niger
3.5.5.1 Hg2+ complete inhibition at 0.1 mM Aspergillus niger
3.5.5.1 iodoacetamide 67% residual activity at 1 mM Aspergillus niger
3.5.5.1 Mg2+ 91% residual activity at 5 mM Aspergillus niger
3.5.5.1 NaN3 88% residual activity at 5 mM Aspergillus niger
3.5.5.1 Ni2+ 44% residual activity at 5 mM Aspergillus niger
3.5.5.1 p-hydroxymercuribenzoate complete inhibition at 1 mM Aspergillus niger
3.5.5.1 Pb2+ 52% residual activity at 0.1 mM Aspergillus niger
3.5.5.1 phenylhydrazine 43% residual activity at 1 mM Aspergillus niger
3.5.5.1 Zn2+ complete inhibition at 5 mM Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 38500
-
 SDS-PAGE, stained with Coomassie Brilliant Blue R-250 Aspergillus niger
3.5.5.1 38560
-
 predicted protein size Aspergillus niger
3.5.5.1 60000
-
 SDS-PAGE, stained with silver nitrate Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Aspergillus niger
-
-
-
3.5.5.1 Aspergillus niger K10
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 ammonium sulfate precipitation, Hi-Prep Sephacryl S-200 gel filtration, and Hi-Prep 16/10 Q-Sepharose column chromatography Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.1 4.9
-
 crude extract, at pH 8.0 and 45°C Aspergillus niger
3.5.5.1 91.6
-
 after 18.7fold purification, at pH 8.0 and 45°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 (R,S)-2-phenylpropionitrile + H2O 1% activity compared to benzonitrile Aspergillus niger ?
-
 ?
3.5.5.1 (R,S)-2-phenylpropionitrile + H2O 1% activity compared to benzonitrile Aspergillus niger K10 ?
-
 ?
3.5.5.1 1,3-dicyanobenzene + H2O 8.4% activity compared to benzonitrile Aspergillus niger ?
-
 ?
3.5.5.1 1,4-dicyanobenzene + H2O 79.5% activity compared to benzonitrile Aspergillus niger ?
-
 ?
3.5.5.1 2 benzonitrile + 3 H2O 100% activity Aspergillus niger benzoic acid + benzamide + NH3
-
 ?
3.5.5.1 2-cyanopyridine + 2 H2O 14.2% activity compared to benzonitrile Aspergillus niger pyridine-2-carboxylic acid + NH3
-
 ?
3.5.5.1 2-phenylacetonitrile + 2 H2O 10.8% activity compared to benzonitrile Aspergillus niger 2-phenylacetic acid + NH3
-
 ?
3.5.5.1 3-chlorobenzonitrile + H2O 41% activity compared to benzonitrile Aspergillus niger 3-chlorobenzoic acid + NH3
-
 ?
3.5.5.1 3-chlorobenzonitrile + H2O 41% activity compared to benzonitrile Aspergillus niger K10 3-chlorobenzoic acid + NH3
-
 ?
3.5.5.1 3-cyanopyridine + 2 H2O 32.4% activity compared to benzonitrile Aspergillus niger pyridine-3-carboxylic acid + NH3
-
 ?
3.5.5.1 3-hydroxybenzonitrile + H2O 8.4% activity compared to benzonitrile Aspergillus niger 3-hydroxybenzoate + NH3
-
 ?
3.5.5.1 3-tolunitrile + H2O 5.5% activity compared to benzonitrile Aspergillus niger 3-methylbenzoic acid + NH3
-
 ?
3.5.5.1 4-chlorobenzonitrile + H2O 29.8% activity compared to benzonitrile Aspergillus niger 4-chlorobenzoate + NH3
-
 ?
3.5.5.1 4-cyanopyridine + 2 H2O 410.7% activity compared to benzonitrile Aspergillus niger pyridine-4-carboxylic acid + NH3
-
 ?
3.5.5.1 4-tolunitrile + H2O 3.4% activity compared to benzonitrile Aspergillus niger 4-methylbenzoic acid + NH3
-
 ?
3.5.5.1 butyronitrile + H2O 17.6% activity compared to benzonitrile Aspergillus niger butyric acid + NH3
-
 ?
3.5.5.1 butyronitrile + H2O 17.6% activity compared to benzonitrile Aspergillus niger K10 butyric acid + NH3
-
 ?
3.5.5.1 propionitrile + H2O 6.9% activity compared to benzonitrile Aspergillus niger propionic acid + NH3
-
 ?
3.5.5.1 propionitrile + H2O 6.9% activity compared to benzonitrile Aspergillus niger K10 propionic acid + NH3
-
 ?
3.5.5.1 thiophen-2-acetonitrile + H2O 56.1% activity compared to benzonitrile Aspergillus niger thiophen-2-ylacetic acid + NH3
-
 ?
3.5.5.1 valeronitrile + H2O 19.6% activity compared to benzonitrile Aspergillus niger valeric acid + NH3
-
 ?
3.5.5.1 valeronitrile + H2O 19.6% activity compared to benzonitrile Aspergillus niger K10 valeric acid + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.1 45
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.1 -70
-
 after five freeze-thaw cycles and subsequent storage for 5 days at -70°C, the activity of the enzyme decreases to about one-fifth of the original activity Aspergillus niger
3.5.5.1 30 55 stable at 30°C, highly active at 48°C (with 92% of maximum activity), but its activity declines sharply at higher temperatures (to 53% and 19% at 50°C and 55°C, respectively), at 30°C and pH 7.2-9.0 the enzyme half-life is about 11 h, at 35°C and 40°C, the half-life decreases to 6.2 h and 2.8 h, respectively Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 8
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.1 7.2 8.5
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.5.1 5.5 10 a sharp activity decrease is observed at slightly acidic pH values, only a trace activity being observed at pH 5.5, 70% and 17% of the activity being retained at pH 9 and 10, respectively Aspergillus niger