EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.215 | DNA and amino acid sequence determination and analysis, expression of wild-type and mutant large subunits | Gluconobacter frateurii |
1.1.99.3 | - |
Gluconobacter oxydans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.215 | membrane | bound | Gluconobacter frateurii | 16020 | - |
1.1.99.3 | membrane | bound to | Gluconobacter oxydans | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.215 | Fe2+ | heme group of cytochrome C bound to the middle subunit | Gluconobacter frateurii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.215 | 23000 | - |
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE | Gluconobacter frateurii |
1.1.1.215 | 44000 | - |
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE | Gluconobacter frateurii |
1.1.1.215 | 65000 | - |
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE | Gluconobacter frateurii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.215 | D-gluconate + NADP+ | Gluconobacter frateurii | - |
2-dehydro-D-gluconate + NADPH | - |
? | |
1.1.1.215 | D-gluconate + NADP+ | Gluconobacter frateurii IFO 3271 | - |
2-dehydro-D-gluconate + NADPH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.215 | Gluconobacter frateurii | A4PIB0 | the large subunit is encoded by gene gndL, the small subunit by gene gndS, and the cytochrome C subunit by gene gndC; gene gndL encoding the large subunit of the enzyme | - |
1.1.1.215 | Gluconobacter frateurii IFO 3271 | A4PIB0 | the large subunit is encoded by gene gndL, the small subunit by gene gndS, and the cytochrome C subunit by gene gndC; gene gndL encoding the large subunit of the enzyme | - |
1.1.99.3 | Gluconobacter oxydans | - |
IFO 3271 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.215 | native enzyme 67fold from membranes and bound to FAD by solubilization with 0.1% Triton X-100, anion chromatography, and three steps of hydrophobic interaction chromatography | Gluconobacter frateurii |
1.1.99.3 | - |
Gluconobacter oxydans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.215 | 137 | - |
purified native enzyme | Gluconobacter frateurii |
1.1.99.3 | 149 | - |
- |
Gluconobacter oxydans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.215 | D-gluconate + NADP+ | - |
Gluconobacter frateurii | 2-dehydro-D-gluconate + NADPH | - |
? | |
1.1.1.215 | D-gluconate + NADP+ | the large subunit is the catalytically active enzyme part, high substrate and regiospecificity | Gluconobacter frateurii | 2-dehydro-D-gluconate + NADPH | - |
? | |
1.1.1.215 | D-gluconate + NADP+ | - |
Gluconobacter frateurii IFO 3271 | 2-dehydro-D-gluconate + NADPH | - |
? | |
1.1.1.215 | D-gluconate + NADP+ | the large subunit is the catalytically active enzyme part, high substrate and regiospecificity | Gluconobacter frateurii IFO 3271 | 2-dehydro-D-gluconate + NADPH | - |
? | |
1.1.99.3 | D-gluconate + ferricyanide | - |
Gluconobacter oxydans | 2-dehydro-D-gluconate + ferrocyanide | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.215 | trimer | 1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE | Gluconobacter frateurii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.215 | 2-keto-D-gluconate-yielding D-gluconate dehydrogenase | - |
Gluconobacter frateurii |
1.1.1.215 | FAD-GADH | - |
Gluconobacter frateurii |
1.1.1.215 | GADH | - |
Gluconobacter frateurii |
1.1.99.3 | FAD-GADH | - |
Gluconobacter oxydans |
1.1.99.3 | flavin adenine dinucleotide-containing GA 2-dehydrogenase | - |
Gluconobacter oxydans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.215 | 25 | - |
assay at | Gluconobacter frateurii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.215 | 5 | - |
assay at | Gluconobacter frateurii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.215 | cytochrome c | bound by the 44 kDa subunit | Gluconobacter frateurii | |
1.1.1.215 | FAD | bound to the enzyme's large 65 kDA subunit | Gluconobacter frateurii | |
1.1.1.215 | NADP+ | - |
Gluconobacter frateurii | |
1.1.99.3 | FAD | - |
Gluconobacter oxydans |