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Literature summary extracted from

  • Toyama, H.; Furuya, N.; Saichana, I.; Ano, Y.; Adachi, O.; Matsushita, K.
    Membrane-bound, 2-keto-D-gluconate-yielding D-gluconate dehydrogenase from Gluconobacter dioxyacetonicus IFO 3271: molecular properties and gene disruption (2007), Appl. Environ. Microbiol., 73, 6551-6556.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.215 DNA and amino acid sequence determination and analysis, expression of wild-type and mutant large subunits Gluconobacter frateurii
1.1.99.3
-
Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.215 membrane bound Gluconobacter frateurii 16020
-
1.1.99.3 membrane bound to Gluconobacter oxydans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.215 Fe2+ heme group of cytochrome C bound to the middle subunit Gluconobacter frateurii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.215 23000
-
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE Gluconobacter frateurii
1.1.1.215 44000
-
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE Gluconobacter frateurii
1.1.1.215 65000
-
1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE Gluconobacter frateurii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.215 D-gluconate + NADP+ Gluconobacter frateurii
-
2-dehydro-D-gluconate + NADPH
-
?
1.1.1.215 D-gluconate + NADP+ Gluconobacter frateurii IFO 3271
-
2-dehydro-D-gluconate + NADPH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.215 Gluconobacter frateurii A4PIB0 the large subunit is encoded by gene gndL, the small subunit by gene gndS, and the cytochrome C subunit by gene gndC; gene gndL encoding the large subunit of the enzyme
-
1.1.1.215 Gluconobacter frateurii IFO 3271 A4PIB0 the large subunit is encoded by gene gndL, the small subunit by gene gndS, and the cytochrome C subunit by gene gndC; gene gndL encoding the large subunit of the enzyme
-
1.1.99.3 Gluconobacter oxydans
-
IFO 3271
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.215 native enzyme 67fold from membranes and bound to FAD by solubilization with 0.1% Triton X-100, anion chromatography, and three steps of hydrophobic interaction chromatography Gluconobacter frateurii
1.1.99.3
-
Gluconobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.215 137
-
purified native enzyme Gluconobacter frateurii
1.1.99.3 149
-
-
Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.215 D-gluconate + NADP+
-
Gluconobacter frateurii 2-dehydro-D-gluconate + NADPH
-
?
1.1.1.215 D-gluconate + NADP+ the large subunit is the catalytically active enzyme part, high substrate and regiospecificity Gluconobacter frateurii 2-dehydro-D-gluconate + NADPH
-
?
1.1.1.215 D-gluconate + NADP+
-
Gluconobacter frateurii IFO 3271 2-dehydro-D-gluconate + NADPH
-
?
1.1.1.215 D-gluconate + NADP+ the large subunit is the catalytically active enzyme part, high substrate and regiospecificity Gluconobacter frateurii IFO 3271 2-dehydro-D-gluconate + NADPH
-
?
1.1.99.3 D-gluconate + ferricyanide
-
Gluconobacter oxydans 2-dehydro-D-gluconate + ferrocyanide
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.215 trimer 1 * 23000 + 1 * 44000 + 1 * 65000, SDS-PAGE Gluconobacter frateurii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.215 2-keto-D-gluconate-yielding D-gluconate dehydrogenase
-
Gluconobacter frateurii
1.1.1.215 FAD-GADH
-
Gluconobacter frateurii
1.1.1.215 GADH
-
Gluconobacter frateurii
1.1.99.3 FAD-GADH
-
Gluconobacter oxydans
1.1.99.3 flavin adenine dinucleotide-containing GA 2-dehydrogenase
-
Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.215 25
-
assay at Gluconobacter frateurii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.215 5
-
assay at Gluconobacter frateurii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.215 cytochrome c bound by the 44 kDa subunit Gluconobacter frateurii
1.1.1.215 FAD bound to the enzyme's large 65 kDA subunit Gluconobacter frateurii
1.1.1.215 NADP+
-
Gluconobacter frateurii
1.1.99.3 FAD
-
Gluconobacter oxydans