BRENDA - Enzyme Database show

Characteristics of Gloeophyllum trabeum alcohol oxidase, an extracellular source of H2O2 in brown rot decay of wood

Daniel, G.; Volc, J.; Filonova, L.; Plihal, O.; Kubatova, E.; Halada, P.; Appl. Environ. Microbiol. 73, 6241-6253 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.3.13
-
Gloeophyllum trabeum
General Stability
EC Number
General Stability
Organism
1.1.3.13
rapid freezing at -80°C and thawing has no significant effect on the enzyme’s activity
Gloeophyllum trabeum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.13
2.3
-
methanol
-
Gloeophyllum trabeum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.3.13
cell wall
the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers
Gloeophyllum trabeum
5618
-
1.1.3.13
extracellular
the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers
Gloeophyllum trabeum
-
-
1.1.3.13
periplasmic space
the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers
Gloeophyllum trabeum
42597
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.13
72400
-
8 * 72400, SDS-PAGE
Gloeophyllum trabeum
1.1.3.13
628000
-
equilibrium ultracentrifugation
Gloeophyllum trabeum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.13
methanol + O2
Gloeophyllum trabeum
possible role for AOX as a major source of H2O2
formaldehyde + H2O2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.3.13
Gloeophyllum trabeum
A8DPS4
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
1.1.3.13
no glycoprotein
-
Gloeophyllum trabeum
Purification (Commentary)
EC Number
Commentary
Organism
1.1.3.13
-
Gloeophyllum trabeum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.3.13
hypha
the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers
Gloeophyllum trabeum
-
1.1.3.13
mycelium
-
Gloeophyllum trabeum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.3.13
12
-
-
Gloeophyllum trabeum
Storage Stability
EC Number
Storage Stability
Organism
1.1.3.13
4°C, 1 week storage of the sterile-filtered purified enzyme in 20 mM sodium phosphate (pH 7.5) results in a 68% loss of activity
Gloeophyllum trabeum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.13
1-butanol + O2
54% of the activity with methanol
684565
Gloeophyllum trabeum
n-butanal + H2O2
-
-
-
?
1.1.3.13
1-pentanol + O2
22% of the activity with methanol
684565
Gloeophyllum trabeum
n-pentanal + H2O2
-
-
-
?
1.1.3.13
1-propanol + O2
73% of the activity with methanol
684565
Gloeophyllum trabeum
n-propanal + H2O2
-
-
-
?
1.1.3.13
2-methyl-1-propanol + O2
7% of the activity with methanol
684565
Gloeophyllum trabeum
butan-2-one + H2O2
-
-
-
?
1.1.3.13
3-methyl-1-butanol + O2
7% of the activity with methanol
684565
Gloeophyllum trabeum
3-methylbutanal + H2O2
-
-
-
?
1.1.3.13
allylalcohol + O2
93% of the activity with methanol
684565
Gloeophyllum trabeum
acrolein + H2O2
-
-
-
?
1.1.3.13
benzyl alcohol + O2
9% of the activity with methanol
684565
Gloeophyllum trabeum
benzaldehyde + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
94% of the activity with methanol
684565
Gloeophyllum trabeum
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
methanol + O2
-
684565
Gloeophyllum trabeum
formaldehyde + H2O2
-
-
-
?
1.1.3.13
methanol + O2
possible role for AOX as a major source of H2O2
684565
Gloeophyllum trabeum
formaldehyde + H2O2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.3.13
octamer
8 * 72400, SDS-PAGE
Gloeophyllum trabeum
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.3.13
15.6
-
methanol
-
Gloeophyllum trabeum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.13
6
10
broad optimum
Gloeophyllum trabeum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.3.13
additional information
-
no activity detectable at pH 5.0 and at pH 12.0
Gloeophyllum trabeum
1.1.3.13
5.5
-
16% of maximal activity
Gloeophyllum trabeum
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.3.13
7
11
pH stability optimum on the alkaline side in the range of pH 7 to 11. 80% of maximum stability at pH 9
Gloeophyllum trabeum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.13
FAD
noncovalently bonded
Gloeophyllum trabeum
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.3.13
Gloeophyllum trabeum
isoelectric focusing
-
5.3
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.3.13
-
Gloeophyllum trabeum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.13
FAD
noncovalently bonded
Gloeophyllum trabeum
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.3.13
rapid freezing at -80°C and thawing has no significant effect on the enzyme’s activity
Gloeophyllum trabeum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.13
2.3
-
methanol
-
Gloeophyllum trabeum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.3.13
cell wall
the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers
Gloeophyllum trabeum
5618
-
1.1.3.13
extracellular
the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers
Gloeophyllum trabeum
-
-
1.1.3.13
periplasmic space
the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers
Gloeophyllum trabeum
42597
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.13
72400
-
8 * 72400, SDS-PAGE
Gloeophyllum trabeum
1.1.3.13
628000
-
equilibrium ultracentrifugation
Gloeophyllum trabeum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.13
methanol + O2
Gloeophyllum trabeum
possible role for AOX as a major source of H2O2
formaldehyde + H2O2
-
-
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
1.1.3.13
no glycoprotein
-
Gloeophyllum trabeum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.3.13
-
Gloeophyllum trabeum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.3.13
hypha
the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers
Gloeophyllum trabeum
-
1.1.3.13
mycelium
-
Gloeophyllum trabeum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.3.13
12
-
-
Gloeophyllum trabeum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.3.13
4°C, 1 week storage of the sterile-filtered purified enzyme in 20 mM sodium phosphate (pH 7.5) results in a 68% loss of activity
Gloeophyllum trabeum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.13
1-butanol + O2
54% of the activity with methanol
684565
Gloeophyllum trabeum
n-butanal + H2O2
-
-
-
?
1.1.3.13
1-pentanol + O2
22% of the activity with methanol
684565
Gloeophyllum trabeum
n-pentanal + H2O2
-
-
-
?
1.1.3.13
1-propanol + O2
73% of the activity with methanol
684565
Gloeophyllum trabeum
n-propanal + H2O2
-
-
-
?
1.1.3.13
2-methyl-1-propanol + O2
7% of the activity with methanol
684565
Gloeophyllum trabeum
butan-2-one + H2O2
-
-
-
?
1.1.3.13
3-methyl-1-butanol + O2
7% of the activity with methanol
684565
Gloeophyllum trabeum
3-methylbutanal + H2O2
-
-
-
?
1.1.3.13
allylalcohol + O2
93% of the activity with methanol
684565
Gloeophyllum trabeum
acrolein + H2O2
-
-
-
?
1.1.3.13
benzyl alcohol + O2
9% of the activity with methanol
684565
Gloeophyllum trabeum
benzaldehyde + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
94% of the activity with methanol
684565
Gloeophyllum trabeum
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
methanol + O2
-
684565
Gloeophyllum trabeum
formaldehyde + H2O2
-
-
-
?
1.1.3.13
methanol + O2
possible role for AOX as a major source of H2O2
684565
Gloeophyllum trabeum
formaldehyde + H2O2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.3.13
octamer
8 * 72400, SDS-PAGE
Gloeophyllum trabeum
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.3.13
15.6
-
methanol
-
Gloeophyllum trabeum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.13
6
10
broad optimum
Gloeophyllum trabeum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.3.13
additional information
-
no activity detectable at pH 5.0 and at pH 12.0
Gloeophyllum trabeum
1.1.3.13
5.5
-
16% of maximal activity
Gloeophyllum trabeum
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.3.13
7
11
pH stability optimum on the alkaline side in the range of pH 7 to 11. 80% of maximum stability at pH 9
Gloeophyllum trabeum
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.3.13
Gloeophyllum trabeum
isoelectric focusing
-
5.3