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Literature summary extracted from

  • Pishtiyski, I.; Popova, V.; Zhekova, B.
    Characterization of cyclodextrin glucanotransferase produced by Bacillus megaterium (2008), Appl. Biochem. Biotechnol., 144, 263-272.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.19 Ag+
-
 Priestia megaterium
2.4.1.19 EDTA
-
 Priestia megaterium
2.4.1.19 Hg+
-
 Priestia megaterium
2.4.1.19 Zn2+
-
 Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.19 additional information
-
 additional information kinetics Priestia megaterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.19 extracellular
-
 Priestia megaterium
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.19 Co2+ activates Priestia megaterium
2.4.1.19 Cu2+ activates Priestia megaterium
2.4.1.19 Mg2+ activates Priestia megaterium
2.4.1.19 Mn2+ activates Priestia megaterium
2.4.1.19 additional information enzyme activity is not affected by Ca2+, Na+, K+, Fe3+, ICH2COOH, and SDS at low concentration Priestia megaterium
2.4.1.19 Sr2+ activates Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.19 68200
-
 gel filtration Priestia megaterium
2.4.1.19 73400
-
 1 * 73400, SDS-PAGE Priestia megaterium
2.4.1.19 75000
-
 about, mass spectrometry Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Priestia megaterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 native enzyme by adsorption on starch, ion exchange chromatography, and gel filtration Priestia megaterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.19 60.39
-
 purified enzyme Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 additional information the enzyme preforms cyclization of different alpha-1,4-glucans, e.g. soluble potato starch, or amylopectin, and amylose, the enzyme forms preferably beta-cyclodextrins, the ratio of products is 27:68:5 for alpha, beta, and gamma cyclodextrins Priestia megaterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.19 monomer 1 * 73400, SDS-PAGE Priestia megaterium

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGTase
-
 Priestia megaterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 60
-
-
 Priestia megaterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.19 20 75 activity range Priestia megaterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 30
-
 stable Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 7.2
-
-
 Priestia megaterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.19 4 11 activity range, about 60% of maximal activity at pH 4.5-9.0 Priestia megaterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.19 6 10.5 stable Priestia megaterium