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Literature summary extracted from

  • Pietzsch, M.; Syldatk, C.; Wagner, F.
    A new racemase for 5-monosubstituted hydantoins (1992), Ann. N. Y. Acad. Sci., 672, 478-483.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.99.5 cysteine slight stimulation Arthrobacter sp.
5.1.99.5 dithiothreitol up to 50% stimulation Arthrobacter sp.
5.1.99.5 EDTA slight stimulation Arthrobacter sp.
5.1.99.5 Sodium dodecyl sulfate slight stimulation Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.99.5 Cu2+ 80% loss of activity Arthrobacter sp.
5.1.99.5 Hg2+ complete loss of activity Arthrobacter sp.
5.1.99.5 iodoacetamide complete loss of activity Arthrobacter sp.
5.1.99.5 p-mercuribenzoate complete loss of activity Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.99.5 21000
-
 4 * 21000, SDS-PAGE Arthrobacter sp.
5.1.99.5 84000
-
 gel filtration Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
5.1.99.5 Arthrobacter sp.
-
 strain DSM 3747
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.99.5
-
 Arthrobacter sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
5.1.99.5 D-5-monosubstituted hydantoin = L-5-monosubstituted hydantoin keto-enol automerism of 5-monosubstituted hydantoins is responsible for racemization Arthrobacter sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.99.5 (5S)-5-(1H-indol-2-ylmethyl)-3-methylimidazolidine-2,4-dione 20.2% of the activity with (5S)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione Arthrobacter sp. (5R)-5-(1H-indol-2-ylmethyl)-3-methylimidazolidine-2,4-dione
-
 r
5.1.99.5 (5S)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione
-
 Arthrobacter sp. (5R)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione
-
 r
5.1.99.5 (5S)-5-(2-methylpropyl)imidazolidine-2,4-dione 9.8% of the activity with (5S)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione Arthrobacter sp. (5S)-5-(2-methylpropyl)imidazolidine-2,4-dione
-
 r
5.1.99.5 (5S)-5-(4-hydroxybenzyl)imidazolidine-2,4-dione 76.7% of the activity with (5S)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione Arthrobacter sp. (5R)-5-(4-hydroxybenzyl)imidazolidine-2,4-dione
-
 r
5.1.99.5 (5S)-5-benzylimidazolidine-2,4-dione 62.7% of the activity with (5S)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione Arthrobacter sp. (5R)-5-benzylimidazolidine-2,4-dione
-
 r
5.1.99.5 (5S)-5-[2-(methylsulfanyl)ethyl]imidazolidine-2,4-dione 20.4% of the activity with (5S)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione Arthrobacter sp. (5R)-5-[2-(methylsulfanyl)ethyl]imidazolidine-2,4-dione
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.99.5 tetramer 4 * 21000, SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.99.5 37
-
-
 Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.99.5 8.5
-
-
 Arthrobacter sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.1.99.5 Arthrobacter sp. isoelectric focusing
-
 4.5