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Literature summary extracted from

  • Chu, X.; Wu, L.; Liu, X.; Li, N.; Li, D.
    Detection of broad-spectrum aminoglycoside antibiotics through fluorescence-labeling aminoglycoside acetyltransferase(6)-Ii (2008), Anal. Biochem., 376, 144-150.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.82 0.0037
-
 tobramycin wild-type enzyme Enterococcus faecium
2.3.1.82 0.0039
-
 neomycin wild-type enzyme Enterococcus faecium
2.3.1.82 0.0082
-
 gentamicin C wild-type enzyme Enterococcus faecium
2.3.1.82 0.0114
-
 kanamycin A wild-type enzyme Enterococcus faecium
2.3.1.82 0.0158
-
 acetyl-CoA wild-type enzyme Enterococcus faecium
2.3.1.82 0.0693
-
 amikacin wild-type enzyme Enterococcus faecium

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.82 Enterococcus faecium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.82 acetyl-CoA + amikacin
-
 Enterococcus faecium CoA + N6'-acetylamikacin
-
 ?
2.3.1.82 acetyl-CoA + gentamicin C
-
 Enterococcus faecium CoA + N6'-acetylgentamicin C
-
 ?
2.3.1.82 acetyl-CoA + kanamycin A
-
 Enterococcus faecium CoA + N6'-acetylkanamycin A
-
 ?
2.3.1.82 acetyl-CoA + neomycin
-
 Enterococcus faecium CoA + N6'-acetylneomycin
-
 ?
2.3.1.82 acetyl-CoA + tobramycin
-
 Enterococcus faecium CoA + N6'-acetyltobramycin
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.82 0.29
-
 amikacin wild-type enzyme Enterococcus faecium
2.3.1.82 0.3
-
 neomycin wild-type enzyme Enterococcus faecium
2.3.1.82 0.8
-
 tobramycin wild-type enzyme Enterococcus faecium
2.3.1.82 1.01
-
 gentamicin C wild-type enzyme Enterococcus faecium
2.3.1.82 1.61
-
 acetyl-CoA wild-type enzyme Enterococcus faecium
2.3.1.82 1.68
-
 kanamycin A wild-type enzyme Enterococcus faecium