Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Katane, M.; Furuchi, T.; Sekine, M.; Homma, H.
    Molecular cloning of a cDNA encoding mouse D-aspartate oxidase and functional characterization of its recombinant proteins by site-directed mutagenesis (2007), Amino Acids, 32, 69-78.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.1 expressed in Escherichia coli strains BL21(DE3)pLysS and JM 109 Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.1 R216A mutant with significantly reduced activity Mus musculus
1.4.3.1 R216E mutant with significantly reduced activity Mus musculus
1.4.3.1 R216L mutant with significantly reduced activity Mus musculus
1.4.3.1 R216M mutant with significantly reduced activity Mus musculus
1.4.3.1 R237A mutant with significantly reduced activity Mus musculus
1.4.3.1 R237E mutant with significantly reduced activity Mus musculus
1.4.3.1 R237Y mutant with significantly reduced activity Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.1 malate 52% inhibition at 50 mM Mus musculus
1.4.3.1 meso-tartrate 52% inhibition 50 mM Mus musculus
1.4.3.1 additional information benzoate has no inhibitory effect Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.1 37620
-
 calculated from amino acid sequence Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.1 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.1 kidney
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.1 D-asparagine + H2O + O2 15% activity compared to D-aspartate Mus musculus ?
-
 ?
1.4.3.1 D-aspartate + H2O + O2 100% activity Mus musculus oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.1 D-glutamate + H2O + O2 14% activity compared to D-aspartate Mus musculus 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.1 D-methionine + H2O + O2 1.8% activity compared to D-aspartate Mus musculus 4-(methylsulfonyl)-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.1 additional information D-alanine,D-proline and L-asparagine are no substrates Mus musculus ?
-
 ?
1.4.3.1 N-methyl-D-aspartate + H2O + O2 83% activity compared to D-aspartate Mus musculus oxaloacetate + CH3NH2 + H2O2
-
 ?
1.4.3.1 N-methyl-L-aspartate + H2O + O2 4% activity compared to D-aspartate Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.1 D-Asp oxidase
-
 Mus musculus
1.4.3.1 DASPO
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.1 FAD dependent Mus musculus
1.4.3.1 FMN 10% activity when FAD is replaced with FMN Mus musculus