Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chaparro-Riggers, J.F.; Rogers, T.A.; Vazquez-Figueroa, E.; Polizzi, K.M.; Bommarius, A.S.
    Comparison of three enoate reductases and their potential use for biotransformations (2007), Adv. Synth. Catal., 349, 1521-1531.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.31 expressed in Escherichia coli BL21(DE3) Yersinia bercovieri
1.3.1.31 expressed in Escherichia coli BL21(DE3) Kluyveromyces lactis
1.3.1.31 expressed in Escherichia coli BL21(DE3) Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.31 2-cyclohexen-1-one the addition of 260 mM 2-cyclohexen-1-one leads to a more than 30fold decrease of the half-life at 30°C Yersinia bercovieri
1.3.1.31 additional information not inhibited by 2-cyclohexanone Yersinia bercovieri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.31 1.24
-
 NADPH at 25°C and pH 7.5 Yersinia bercovieri

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.31 Kluyveromyces lactis P40952 strain ATCC 8585D-5
-
1.3.1.31 Pseudomonas putida Q9R9V9 xenobiotic reductase A showing enoate reductase activity
-
1.3.1.31 Yersinia bercovieri
-
 strain ATCC 43970
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.31 Ni2+-NTA bead chromatography Yersinia bercovieri
1.3.1.31 Ni2+-NTA bead chromatography Kluyveromyces lactis
1.3.1.31 Ni2+-NTA bead chromatography Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.31 2.46
-
 highest specific activity with 10 mM N-ethylmaleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25°C Kluyveromyces lactis
1.3.1.31 15.81
-
 highest specific activity with 10 mM maleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25°C Yersinia bercovieri
1.3.1.31 25.82
-
 highest specific activity with 10 mM maleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.31 (R)-carvone + NADPH
-
 Yersinia bercovieri 2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+
-
 ?
1.3.1.31 (R)-carvone + NADPH
-
 Kluyveromyces lactis 2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+
-
 ?
1.3.1.31 (R)-carvone + NADPH
-
 Pseudomonas putida 2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+
-
 ?
1.3.1.31 2-cyclohexen-1-one + NADH
-
 Yersinia bercovieri 2-cyclohexanone + NAD+
-
 ?
1.3.1.31 2-cyclohexen-1-one + NADH
-
 Kluyveromyces lactis 2-cyclohexanone + NAD+
-
 ?
1.3.1.31 2-cyclohexen-1-one + NADH
-
 Pseudomonas putida 2-cyclohexanone + NAD+
-
 ?
1.3.1.31 3-methyl-2-butenal + NADPH
-
 Yersinia bercovieri 3-methylbutanal + NADP+
-
 ?
1.3.1.31 3-methyl-2-butenal + NADPH
-
 Kluyveromyces lactis 3-methylbutanal + NADP+
-
 ?
1.3.1.31 3-methyl-2-butenal + NADPH
-
 Pseudomonas putida 3-methylbutanal + NADP+
-
 ?
1.3.1.31 but-3-en-2-one + NADPH
-
 Yersinia bercovieri butan-2-one + NADP+
-
 ?
1.3.1.31 but-3-en-2-one + NADPH
-
 Kluyveromyces lactis butan-2-one + NADP+
-
 ?
1.3.1.31 but-3-en-2-one + NADPH
-
 Pseudomonas putida butan-2-one + NADP+
-
 ?
1.3.1.31 cinnamaldehyde + NADPH
-
 Yersinia bercovieri ?
-
 ?
1.3.1.31 cinnamaldehyde + NADPH
-
 Kluyveromyces lactis ?
-
 ?
1.3.1.31 cinnamaldehyde + NADPH
-
 Pseudomonas putida ?
-
 ?
1.3.1.31 citral + NADPH
-
 Yersinia bercovieri 3-phenylpropanal + NADP+
-
 ?
1.3.1.31 citral + NADPH
-
 Kluyveromyces lactis 3-phenylpropanal + NADP+
-
 ?
1.3.1.31 citral + NADPH
-
 Pseudomonas putida 3-phenylpropanal + NADP+
-
 ?
1.3.1.31 ethyl 3-(tetrahydrofuran)propanoate + NADP+
-
 Yersinia bercovieri ? + NADPH
-
 ?
1.3.1.31 ethyl 3-(tetrahydrofuran)propanoate + NADPH
-
 Kluyveromyces lactis ? + NADP+
-
 ?
1.3.1.31 ethyl 3-(tetrahydrofuran)propanoate + NADPH
-
 Pseudomonas putida ? + NADP+
-
 ?
1.3.1.31 ketoisophorone + NADPH
-
 Yersinia bercovieri (6R)-levodione
-
 ?
1.3.1.31 ketoisophorone + NADPH
-
 Kluyveromyces lactis (6R)-levodione
-
 ?
1.3.1.31 ketoisophorone + NADPH
-
 Pseudomonas putida (6R)-levodione
-
 ?
1.3.1.31 maleic anhydride + NADPH
-
 Yersinia bercovieri dihydrofuran-2,5-dione + NADP+
-
 ?
1.3.1.31 maleic anhydride + NADPH
-
 Kluyveromyces lactis dihydrofuran-2,5-dione + NADP+
-
 ?
1.3.1.31 maleic anhydride + NADPH
-
 Pseudomonas putida dihydrofuran-2,5-dione + NADP+
-
 ?
1.3.1.31 maleimide + NADH + H+
-
 Yersinia bercovieri pyrrolidine-2,5-dione + NAD+
-
 ?
1.3.1.31 maleimide + NADH + H+
-
 Kluyveromyces lactis pyrrolidine-2,5-dione + NAD+
-
 ?
1.3.1.31 maleimide + NADH + H+
-
 Pseudomonas putida pyrrolidine-2,5-dione + NAD+
-
 ?
1.3.1.31 additional information NADPH does not react at all in the presence of 2-cyclohexen-1-one and rarely in presence of maleimide Yersinia bercovieri ?
-
 ?
1.3.1.31 additional information NADPH does not react at all in the presence of 2-cyclohexen-1-one and rarely in presence of maleimide Kluyveromyces lactis ?
-
 ?
1.3.1.31 additional information NADPH does not react at all in the presence of 2-cyclohexen-1-one and rarely in presence of maleimide Pseudomonas putida ?
-
 ?
1.3.1.31 N-ethylmaleimide + NADPH
-
 Yersinia bercovieri N-ethylpyrrolidine-2,5-dione + NADP+
-
 ?
1.3.1.31 N-ethylmaleimide + NADPH
-
 Kluyveromyces lactis N-ethylpyrrolidine-2,5-dione + NADP+
-
 ?
1.3.1.31 N-ethylmaleimide + NADPH
-
 Pseudomonas putida N-ethylpyrrolidine-2,5-dione + NADP+
-
 ?
1.3.1.31 trans,trans-2,4-hexadienal + NADPH
-
 Yersinia bercovieri ?
-
 ?
1.3.1.31 trans,trans-2,4-hexadienal + NADPH
-
 Kluyveromyces lactis ?
-
 ?
1.3.1.31 trans,trans-2,4-hexadienal + NADPH
-
 Pseudomonas putida ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.31 enoate reductase
-
 Yersinia bercovieri
1.3.1.31 enoate reductase
-
 Kluyveromyces lactis
1.3.1.31 enoate reductase
-
 Pseudomonas putida
1.3.1.31 KYE1
-
 Kluyveromyces lactis
1.3.1.31 NADPH dehydrogenase 1
-
 Kluyveromyces lactis
1.3.1.31 old yellow enzyme
-
 Yersinia bercovieri
1.3.1.31 old yellow enzyme
-
 Kluyveromyces lactis
1.3.1.31 old yellow enzyme
-
 Pseudomonas putida
1.3.1.31 XenA xenobiotic reductase A showing enoate reductase activity toward 2-cyclohexen-1-one Pseudomonas putida
1.3.1.31 Yers-ER
-
 Yersinia bercovieri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.31 30 45 KYE1 has a half-life of 1146 min at 30°C, 210 min at 37°C, and less than 5 min at 45°C Kluyveromyces lactis
1.3.1.31 30 45 XenA has a half-life of 600 min at 30°C, 99 min at 37°C, and less than 5 min at 45°C Pseudomonas putida
1.3.1.31 30 45 Yers-ER has a half-life of more than 2160 min at 30°C, 1488 min at 37°C, and 117 min at 45°C Yersinia bercovieri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.31 1.97
-
 NADPH at 25°C and pH 7.5 Yersinia bercovieri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.31 7
-
-
 Yersinia bercovieri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.31 6 8.5
-
 Yersinia bercovieri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.31 5 9.5 the activity drops off sharply between pH 5-6 and pH 8.5-9.5 Yersinia bercovieri

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.31 NADH the enzymes shows very little or no activity with NADH Yersinia bercovieri
1.3.1.31 NADH the enzymes shows very little or no activity with NADH Kluyveromyces lactis
1.3.1.31 NADH the enzymes shows very little or no activity with NADH Pseudomonas putida
1.3.1.31 NADPH preferred cofactor Yersinia bercovieri
1.3.1.31 NADPH preferred cofactor Kluyveromyces lactis
1.3.1.31 NADPH preferred cofactor Pseudomonas putida