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Literature summary extracted from
- Polyphenol oxidase activity in dormant saffron (Crocus sativus L.) corm (2007), Acta Physiol. Plant., 29, 463-471.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | kojic acid | - |
Crocus sativus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.3 | - |
pyrogallol | detected in dormant saffron corms | Crocus sativus | |
| 1.14.18.1 | 2 | 3 | catechol | detected in dormant saffron corms | Crocus sativus | |
| 1.14.18.1 | 4.2 | - |
L-Dopa | detected in dormant saffron corms | Crocus sativus | |
| 1.14.18.1 | 130 | - |
p-Cresol | detected in dormant saffron corms | Crocus sativus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 39000 | - |
dormant saffron corm extracts are submitted to non-denaturing polyacrylamide gel electrophoresis, staining for PPO activity revealed three distinct bands 39000, 60000, 15000 Da | Crocus sativus |
| 1.14.18.1 | 60000 | - |
dormant saffron corm extracts are submitted to non-denaturing polyacrylamide gel electrophoresis, staining for PPO activity revealed three distinct bands 39000, 60000, 15000 Da | Crocus sativus |
| 1.14.18.1 | 150000 | - |
dormant saffron corm extracts are submitted to non-denaturing polyacrylamide gel electrophoresis, staining for PPO activity revealed three distinct bands 39000, 60000, 15000 Da | Crocus sativus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Crocus sativus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.18.1 | partial purification of PPO from dormant saffron corms is carried out by ammonium sulfate fractionation (between 30 and 80% ammonium sulfate) followed by dialysis against phosphate buffer 0.1 M, pH 7.00, 0.02% PMSF, then chromatography through CM-Sephadex C-25 equilibrated with the same buffer | Crocus sativus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 27 | - |
L-dopa, detected in dormant saffron corms | Crocus sativus |
| 1.14.18.1 | 45 | - |
pyrogallol, detected in dormant saffron corms | Crocus sativus |
| 1.14.18.1 | 600 | - |
p-cresol, detected in dormant saffron corms | Crocus sativus |
| 1.14.18.1 | 900 | - |
catechol, detected in dormant saffron corms | Crocus sativus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | 4-methylcatechol + 1/2 O2 | - |
Crocus sativus | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
| 1.14.18.1 | catechol + 1/2 O2 | - |
Crocus sativus | 1,2-benzoquinone + H2O | - |
? | |
| 1.14.18.1 | L-dopa + 1/2 O2 | - |
Crocus sativus | L-dopaquinone + H2O | - |
? | |
| 1.14.18.1 | additional information | no activity is detectable with L-tyrosine, tyramine or phenol as substrate | Crocus sativus | ? | - |
? | |
| 1.14.18.1 | p-cresol + O2 + AH2 | - |
Crocus sativus | 4-methylcatechol + H2O + A | - |
? | |
| 1.14.18.1 | pyrogallol + 1/2 O2 | - |
Crocus sativus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | monophenol, o-diphenol:O2 oxidoreductase | - |
Crocus sativus |
| 1.14.18.1 | polyphenol oxidase | EC1.14.18.1, EC1.10.3.1 | Crocus sativus |
| 1.14.18.1 | PPO | - |
Crocus sativus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 22 | 25 | assays are conducted at room tempertature | Crocus sativus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 4.5 | - |
two pH optima, respectively at 4.5 and 6.7, are observed with all substrates and a third one, at 8.5, is found with L-dopa and p-cresol | Crocus sativus |
| 1.14.18.1 | 6.7 | - |
two pH optima, respectively at 4.5 and 6.7, are observed with all substrates and a third one, at 8.5, is found with L-dopa and p-cresol | Crocus sativus |
| 1.14.18.1 | 8.5 | - |
two pH optima, respectively at 4.5 and 6.7, are observed with all substrates and a third one, at 8.5, is found with L-dopa and p-cresol | Crocus sativus |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.22 | - |
with catechol as substrate, competitive inhibition | Crocus sativus | kojic acid | |
| 1.14.18.1 | 0.35 | - |
with pyrogallol as substrate, mixed inhibition | Crocus sativus | kojic acid | |
| 1.14.18.1 | 0.5 | - |
with p-cresol as substrate, uncompetitive inhibition | Crocus sativus | kojic acid | |
| 1.14.18.1 | 0.7 | - |
with L-dopa as substrate, noncompetitive inhibition | Crocus sativus | kojic acid | |
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